Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells

Gerloff, Kirsten; Albrecht, Catrin; Boots, Agnes W.; Förster, Irmgard; Schins, Roel P. F.

doi:10.1080/17435390903276933

Cited by 33 publications

(46 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, study investigated the cytotoxic and DNA damaging properties of amorphous fumed nano-silica (14 nm) in the human colon epithelial cell-line, Caco-2. Exposure to nano-silica for up to 24 h caused cell mortality, significant DNA damage, and total GSH depletion 28 .…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity of nano-silica in mammalian cell lines

Choi

Kim

Song

et al. 2011

Toxicol. Environ. Health Sci.

View full text Add to dashboard Cite

Nanomaterials are defined by the U.S. National Nanotechnology Initiative as materials that have at least one dimension in the 1-to 100-nm range. Due to their unique physical and chemical characteristics, nanotechnology has become one of the leading technologies over the past 10 years. This study represents data on genotoxic effects of nanoparticles and their application for assessing human health risks. Silica (SiO 2 ) is a multi-functional ceramic material that is being used in various industries to improve surfaces and mechanical properties of diverse materials, such as paints and coatings, plastics, synthetic rubber, adhesives, sealants, or insulation materials. However, recent studies have shown that nano-sized silica (nano-silica) (10 nm in diameter) can generate adverse effects, like liver injury and inflammation. The cytotoxicity and genotoxicity of nano-silica were investigated using the dye exclusion assay, comet assay, and mouse lymphoma thymidine kinase (tk +/-) mouse lymphoma assay (MLA). IC 20 of nano-silica in L5178Y cells was determined to be of 2,441.41 μg/mL and 2,363.28 μg/mL without and with S-9, respectively. Also IC 20 of nano-silica in BEAS-2B cells was determined to be of 2,324.23 μg/mL and 537.11 μg/mL without and with S-9, respectively. In the comet assay, treating L5178Y cells and BEAS-2B cells with nanosilica treatment induced approximately 2-fold increases in tail moment (P⁄0.05) without and with S-9. Also, the mutant frequencies in the nano-silica treated L5178Y cells were not significantly increased compared to the solvent controls. The results of this study indicate that nano-silica can cause primary DNA damage and cytotoxicity but not mutagenicity in cultured mammalian cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Genotoxicity of nano-silica in mammalian cell lines

Choi

Kim

Song

et al. 2011

Toxicol. Environ. Health Sci.

View full text Add to dashboard Cite

show abstract

“…In contrast, comet assays performed in HT29, HaCat and A549 cell lines exposed to colloidal Ludox SM-30 1 (14 nm) at 0.1, 1 and 10 mg mL À1 for 24 h showed an increase in DNA damage with a dose-response relationship for all cell lines (Mu et al, 2012). A pyrogenic SAS from SigmaAldrich (14 nm) responded positively in comet assays performed in the Caco-2 cell line following 4 h treatment with SAS at 20 mg cm À2 (Gerloff et al, 2009). …”

Section: Introductionmentioning

confidence: 90%

Cytotoxic and genotoxic evaluation of different synthetic amorphous silica nanomaterials in the V79 cell line

et al. 2016

View full text Add to dashboard Cite

The nature of occupational risks and hazards in industries that produce or use synthetic amorphous silica (SAS) nanoparticles is still under discussion. Manufactured SAS occur in amorphous form and can be divided into two main types according to the production process, namely, pyrogenic silica (powder) and precipitated silica (powder, gel or colloid). The physical and chemical properties of SAS may vary in terms of particle size, surface area, agglomeration state or purity, and differences in their toxicity potential might therefore be expected. The aim of this study was to compare the cytotoxicity and genotoxicity of representative manufactured SAS samples in Chinese hamster lung fibroblasts (V79 cells). Five samples from industrial SAS producers were evaluated, that is, two pyrogenic SAS powders (with primary particle sizes of 20 nm and 25/70 nm), one precipitated SAS powder (20 nm) and two precipitated SAS colloids (15 and 40/80 nm). V79 cell cultures were treated with different concentrations of SAS pre-dispersed in bovine serum albumin -water medium. Pyr (pyrogenic) 20, Pre (precipitated) 20 and Col (colloid) 15 significantly decreased the cell viability after 24 h of exposure, whilst Pyr 25/70 and Col 40/80 had negligible effects. The cytotoxicity of Pyr 20, Pre 20 and Col 15 was revealed by the induction of apoptosis, and Pyr 20 and Col 15 also produced DNA damage. However, none of the SAS samples generated intracellular reactive oxidative species, micronuclei or genomic mutations in V79 cells after 24 h of exposure. Overall, the results of this study show that pyrogenic, precipitated and colloidal manufactured SAS of around 20 nm primary particle size can produce significant cytotoxic and genotoxic effects in V79 cells. In contrast, the coarser-grained pyrogenic and colloid SAS (approximately 50 nm) yielded negligible toxicity, despite having been manufactured by same processes as their finer-grained equivalents. To explain these differences, the influence of particle agglomeration and oxidative species formation is discussed.

show abstract

“…Whether this would be sufficient to perturb lysosomal structure is unclear but certainly Gerloff et al have preliminary evidence indicating that nano-sized (w14 nm diameter) amorphous silica invokes some toxicity in epithelial cells [10]. Such considerations are important in light of nanoparticulate, amorphous silica being introduced to the USA diet [11] that the company claims to be sized at 4e6 nm diameter [12].…”

Section: The Nano Revolutionmentioning

confidence: 99%