Cytotoxicity and Microbicidal Activity of Commonly Used Organic Solvents: A Comparative Study and Application to a Standardized Extract from Vaccinium macrocarpon

Ilieva, Yana; Dimitrova, Lyudmila; Zaharieva, Maya M.; Kaleva, Mila; Alov, Petko; Tsakovska, Ivanka; Pencheva, Tania; Tibi, Ivanka Pencheva-El; Najdenski, Hristo; Pajeva, Ilza

doi:10.3390/toxics9050092

Cited by 25 publications

(25 citation statements)

References 28 publications

(49 reference statements)

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“…The skin cell lines were purchased from CLS Cell Lines Service (GmbH, Eppelheim, Germany), and HEP-G2 cells were obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen, German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany). Maintenance of cells followed the protocols described previously in [97].…”

Section: Cell Lines and Culture Conditionsmentioning

confidence: 99%

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Redox-Modulating Capacity and Antineoplastic Activity of Wastewater Obtained from the Distillation of the Essential Oils of Four Bulgarian Oil-Bearing Roses

et al. 2021

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The wastewater from the distillation of rose oils is discharged directly into the soil because it has a limited potential for future applications. The aim of the present study was to determine in vitro the chromatographic profile, redox-modulating capacity, and antineoplastic activity of wastewater obtained by distillation of essential oils from the Bulgarian Rosa alba L., Rosa damascena Mill., Rosa gallica L., and Rosa centifolia L. We applied UHPLC-HRMS for chromatographic analysis of rose wastewaters, studied their metal-chelating and Fe(III)-reducing ability, and performed MTT assay for the evaluation of cytotoxic potential against three tumorigenic (HEPG2—hepatocellular adenocarcinoma, A-375—malignant melanoma, A-431—non-melanoma epidermoid squamous skin carcinoma) and one non-tumorigenic human cell lines (HaCaT—immortalized keratinocytes). The median inhibitory concentrations (IC50) were calculated with nonlinear modeling using the MAPLE® platform. The potential of the wastewaters to induce apoptosis was also examined. Mono-, di-, and acylated glycosides of quercetin and kaempferol, ellagic acid and its derivatives as main chemical components, and gallic acid and its derivatives—such as catechin and epicatechin—were identified. The redox-modulating capacity of the samples (TPTZ test) showed that all four wastewaters exhibited the properties of excellent heavy metal cleaners, but did not exert very strong cytotoxic effects. The lowest IC50 rate was provided in wastewater from R. centifolia (34–35 µg/mL of gallic acid equivalents after a 72 h period for all cell lines). At 24 and 48 hours, the most resistant cell line was HEPG2, followed by HaCaT. After 72 h of exposure, the IC50 values were similar for tumor and normal cells. Still, R. damascena had a selectivity index over 2.0 regarding A-431 non-melanoma skin cancer cells, showing a good toxicological safety profile in addition to moderate activity—IC50 of 35 µg/mL polyphenols. The obtained results related to wastewaters acquired after the distillation of essential oils from the Bulgarian R. alba, R. damascena, R. gallica, and R. centifolia direct our attention to further studies for in-depth elucidation of their application as detoxifying agents under oxidative damage conditions in other experimental datasets.

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Section: Cell Lines and Culture Conditionsmentioning

confidence: 99%

“…MTT assay was executed according to Annex C, ISO 10993-5 (ISO 10993-5:2009) for the evaluation of cytotoxic activity, as previously described [97], but there was exposure after treatment for 24 and 48 h in addition to the incubation for 72 h.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Redox-Modulating Capacity and Antineoplastic Activity of Wastewater Obtained from the Distillation of the Essential Oils of Four Bulgarian Oil-Bearing Roses

et al. 2021

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“…In this sense, the recommended vehicles for solubilization of curcumin by the manufacturer are ethanol and dimethyl sulfoxide (DMSO). Although in many papers the effects of the solvents on culture viability are not shown [37,49,70] (or are not discussed [47,71,72]), if they do not present cytotoxic effects, the reality is that both vehicles produce effects that vary widely depending on the cell type and duration of treatment [71,[73][74][75]. The pre-established idea of the absence of the effects of these vehicles is recurrent in the literature.…”

Section: Discussionmentioning

confidence: 99%

Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture

Alberdi¹,

Rosso²,

Velóz³

et al. 2022

Preprint

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Charcot-Marie-Tooth (CMT) syndrome is the most common progressive human motor and sensory peripheral neuropathy. CMT type 1E is a demyelinating neuropathy affecting Schwann cells due to pmp22 mutations, modelized by Trembler-J mice. Curcumin, a natural polyphenol com-pound obtained from turmeric (Curcuma longa), exhibits dose- and time-varying antitumor, antioxidant and neuroprotective properties, however, the neurotherapeutic actions of curcumin still remain elusive. Here, we propose curcumin as a possible natural treatment capable of enhancing cellular detoxification mechanisms, resulting in an improvement of the neurodegenerative Trembler-J phenotype. Using a refined method for obtaining enriched Schwann cell cultures, we evaluated the neurotherapeutic action of low dose curcumin treatment on the PMP22 expression, and on the chaperones (HSF1 and Hsp27) and autophagy/mTOR (HDAC6 and ribosomes) pathways in Trembler-J and wild-type genotypes. In wild-type Schwann cells, the action of cur-cumin resulted in strong stimulation of the chaperone and macroautophagy pathway, whereas the modulation of ribophagy showed a mild effect. However, despite the promising neuroprotective effects for the treatment of neurological diseases, we report (demonstrate?) that the action of cur-cumin in Trembler-J Schwann cells could be impaired due to the irreversible impact of ethanol used as a common curcumin vehicle necessary for administration. These results contribute to expand our still limited understanding of PMP22 biology in neurobiology, and expose the intrinsic lability of the neurodegenerative Trembler-J genotype. Furthermore, they unravel interesting physiological mechanisms of cellular resilience relevant for to the pharmacological treatment of the neurodegenerative Tremble J phenotype with curcumin and ethanol. We conclude that the analysis of the effects of the vehicle itself is an essential and in-escapable step to comprehensibly assess the own effects and full potential of curcumin treatment for therapeutic purposes.

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“…We first examine the cytotoxic effect of P. pterocarpum leaf extracts against B16-F1 mouse melanocyte cells. According to ISO 10993-5, any substance is regarded to have cytotoxic potential if the cell viability percentage is reduced to less than 70% of the untreated control (Ilieva et al, 2021;Nogueira et al, 2021). In this study, we found that both P. pterocarpum extracts did not exhibit any cytotoxic effect against B16-F1 mouse melanocyte cells (Figure 1) up to 4000 ng/mL.…”

Section: Discussionmentioning

confidence: 99%

IN-VITRO MELANOGENESIS, CYTOTOXICITY AND ANTIOXIDANT ACTIVITIES OF Peltophorum pterocarpum LEAF EXTRACTS

SHAFIE¹,

RASHID²,

Masilamani³

et al. 2022

MAB

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Melanin is a protective pigment against cellular damage and skin cancer. Peltophorum pterocarpum has been used for centuries to treat skin diseases like eczema and psoriasis. However, whether P. pterocarpum leaf extract can affect melanin synthesis with the advantage of antioxidant defense against ultraviolet (UV) radiation remains to be investigated. We aimed to examine the melanogenesis, cytotoxicity, and antioxidant activities of P. pterocarpum leaf extracts. A two-dimensional (2D) cell culture model was employed to demonstrate the effect of P. pterocarpum extracts on melanin synthesis. The cell viability of B16-F1 melanoma cells was measured by Neutral Red Uptake (NRU) assay. Antioxidant activity was accessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) assays. The ethanol extract of P. pterocarpum dose-dependently increased the melanin content and displayed cytotoxicity to B16-F1 melanoma cells at the highest concentration. In contrast, the equal amounts of the aqueous extract significantly inhibited melanin synthesis and did not show any cytotoxic effects on B16-F1 melanoma cells. The P. pterocarpum ethanol extract had a significantly higher total phenolic and flavonoid content than the aqueous extract and was more effective at scavenging DPPH free radicals and intracellular ROS induced by UVB, with IC50 of 519.24 ± 122.57 vs 1798.45 ± 143.09 µg/mL and 878.00 ± 23.50 vs 1379.00 ± 21.81 µg/mL, respectively. These findings suggest that the pro-melanogenic and anti-melanogenic activities of P. pterocarpum extract with antioxidant capacity against UVB-induced cellular damage are affected by the total phenolic and flavonoid contents.

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Cytotoxicity and Microbicidal Activity of Commonly Used Organic Solvents: A Comparative Study and Application to a Standardized Extract from Vaccinium macrocarpon

Cited by 25 publications

References 28 publications

Redox-Modulating Capacity and Antineoplastic Activity of Wastewater Obtained from the Distillation of the Essential Oils of Four Bulgarian Oil-Bearing Roses

Redox-Modulating Capacity and Antineoplastic Activity of Wastewater Obtained from the Distillation of the Essential Oils of Four Bulgarian Oil-Bearing Roses

Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture

IN-VITRO MELANOGENESIS, CYTOTOXICITY AND ANTIOXIDANT ACTIVITIES OF Peltophorum pterocarpum LEAF EXTRACTS

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