Cytotoxicity and genotoxicity of nanosilver in stable <i>GADD45α</i> promoter‐driven luciferase reporter HepG2 and A549 cells

Che, Bizhong; Luo, Qian; Zhai, Bingzhong; Fan, Guoqiang; Li, Yong; Cheng, Kaiming; Xin, Lili

doi:10.1002/tox.22433

Cited by 11 publications

(7 citation statements)

References 41 publications

(63 reference statements)

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“…AgNPs attach to the cell membrane where they alter permeability and penetrate the cell, thus releasing Ag+ ions. Once inside the cell, Ag+ ions cause damage to structures and biomolecules, alterations in respiration, oxidative stress by radical oxygen species (ROS) and modulation of signal transduction pathways [109,110] The principal mechanism through which NPs cause cell damage, including DNA damage, is based on the exogenous generation of ROS such as superoxide anion, hydroxyl radical, singlet oxygen, hypochlorous acid and hydrogen peroxide which can damage DNA and lead to mutagenesis processes [24,83,103,111,112].AgNPs can also interact with mitochondria and disrupt the electron transport chain, thereby causing ROS production and interrupting of ATP synthesis; ROS increase and a shortage of ATP result in protein and DNA damage [112,113].…”

Section: Discussionmentioning

confidence: 99%

“…In all studies, the results were statistically evaluated by comparing treated cells to untreated control cells; those with p values of <0.05 were considered positive. Some of the in vitro studies studied the uptake of AgNPs inside the cells, which is an essential determination to prove that there has been contact between the cell organelles and the AgNPs [53,72,73,77,[81][82][83]88,89,91,92,98]. Uptake was demonstrated through several methods such as transmission electronic microscopy (TEM), side scatter (SSC) intensity analysis by flow cytometry, confocal microscopy, Raman spectroscopy and scanning transmission electron microscopy (STEM).…”

Section: In Vitro Studiesmentioning

confidence: 99%

“…Moreover, it is worth noting that AgNPs interactions with the cell membrane can also produce toxic effects, mainly due to oxidative stress. Several authors have studied the influence that the release of Ag+ ions may have on AgNPs genotoxicity; some concluded that that their role is negligible, whereas others obtained inconclusive results or indirect evidence of their influence on the AgNPs cytotoxicity and genotoxicity [74,77,82,83].…”

Section: In Vitro Studiesmentioning

confidence: 99%

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Genotoxicity of Silver Nanoparticles

et al. 2020

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Silver nanoparticles (AgNPs) are widely used in diverse sectors such as medicine, food, cosmetics, household items, textiles and electronics. Given the extent of human exposure to AgNPs, information about the toxicological effects of such products is required to ensure their safety. For this reason, we performed a bibliographic review of the genotoxicity studies carried out with AgNPs over the last six years. A total of 43 articles that used well-established standard assays (i.e., in vitro mouse lymphoma assays, in vitro micronucleus tests, in vitro comet assays, in vivo micronucleus tests, in vivo chromosome aberration tests and in vivo comet assays), were selected. The results showed that AgNPs produce genotoxic effects at all DNA damage levels evaluated, in both in vitro and in vivo assays. However, a higher proportion of positive results was obtained in the in vitro studies. Some authors observed that coating and size had an effect on both in vitro and in vivo results. None of the studies included a complete battery of assays, as recommended by ICH and EFSA guidelines, and few of the authors followed OECD guidelines when performing assays. A complete genotoxicological characterization of AgNPs is required for decision-making.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Studiesmentioning

confidence: 99%

Section: In Vitro Studiesmentioning

confidence: 99%

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Genotoxicity of Silver Nanoparticles

et al. 2020

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“…AgNPs have been shown to induce DNA damage, cell death by apoptosis and necrosis, and also cell cycle arrest in different cell lines [1]. The effects on the cell cycle seem to be cell type-dependent; in some studies, S-phase arrest has been reported, whereas others point to G2-phase arrest [1,23,44,49,50,51,52]. In our study, A549 and Calu-1 cells showed accumulation in the G2-phase after AgNP exposure, whereas the BEAS-2B cells accumulated in the S-phase.…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles Induce Mitochondrial Protein Oxidation in Lung Cells Impacting Cell Cycle and Proliferation

et al. 2019

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Silver nanoparticles (AgNPs) are widely used nanomaterials in both commercial and clinical biomedical applications, due to their antibacterial properties. AgNPs are also being explored for the treatment of cancer in particular in combination with ionizing radiation. In this work, we studied the effects of AgNPs and ionizing radiation on mitochondrial redox state and function in a panel of lung cell lines (A549, BEAS-2B, Calu-1 and NCI-H358). The exposure to AgNPs caused cell cycle arrest and decreased cell proliferation in A549, BEAS-2B and Calu-1, but not in NCI-H358. The mitochondrial reactive oxygen species (ROS) and protein oxidation increased in a time- and dose-dependent manner in the more sensitive cell lines with the AgNP exposure, but not in NCI-H358. While ionizing radiation also induced changes in the mitochondrial redox profiles, in general, these were not synergistic with the effects of AgNPs with the exception of NCI-H358 and only at a higher dose of radiation.

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“…Among many reasons of Ag NP genotoxicity, one include the toxic effect produce by Ag NPs due to oxidative stress while their interactions with cell membrane [31]. Several reports have mention that the release of Ag+ ions may have influence on Ag NPs genotoxicity; some studied that their role is negligible, while others obtained indirect or inconclusive evidence of their influence on the Ag NPs genotoxicity [32][33][34][35]. A few studies have reported the amount of ROS generation may enhanced by intracellular Ag NPs [36,37] which cause multiple intracellular effects, including DNA damage leading to mutagenesis [38].…”

Section: Introductionmentioning

confidence: 99%

In-vitro Study on Genotoxicity of Green Synthesized Silver Nanoparticles

Mecwan¹,

Das²,

Thakore³

et al. 2021

Nano BioMed ENG

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The wide use of metal nanoparticles has raised the concern of their release in the environment and their biological safety. The use of silver nanoparticles (Ag NPs) as effective antimicrobial agent is also questioned due to the resistance developed by pathogens and the biosafety for the host. The genotoxicity caused by such particles need to be assessed to ensure that they are safe to use. In this study, green synthesized Ag NPs were used and the biosafety in terms of genotoxicity was evaluated by in vitro Chromosome aberration (CA) assay using short-term cultures of human peripheral blood. The physicochemical parameters of these Ag NPs were studied for stability in nano form. The transmission electron microscopy (TEM) and energy-dispersive spectroscopy (EDS) were carried out. The dynamic light scattering and zeta potential measurements revealed stable nano particles. The UV visible & fluorescence spectroscopy for binding affinity of Ag NPs did not suggest significant DNA interaction. The exposure of whole blood cultures to Ag NPs for 24 h showed dose dependent increase in frequency of CA per cell at 350, 650 and 1000 µL (P = NS, P < 0.05 and P < 0.001 respectively). Our study demonstrates that genotoxicity of Ag NPs can be reduced by the use of green synthesized Ag NPs at low dose exposure, which will guide selecting right concentrations of NP for further in vivo studies, and future applications.

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Cytotoxicity and genotoxicity of nanosilver in stable GADD45α promoter‐driven luciferase reporter HepG2 and A549 cells

Cited by 11 publications

References 41 publications

Genotoxicity of Silver Nanoparticles

Genotoxicity of Silver Nanoparticles

Silver Nanoparticles Induce Mitochondrial Protein Oxidation in Lung Cells Impacting Cell Cycle and Proliferation

In-vitro Study on Genotoxicity of Green Synthesized Silver Nanoparticles

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