Cytotoxicity and Genotoxicity of Bovine Pericardium Preserved in Glycerol

Rodas, Andrea C.D.; Maizato, Marina Junko Shiotsu; Leirner, Adolfo A.; Pitombo, Ronaldo Nogueira de Moraes; Polakiewicz, Bronislaw; Beppu, Marisa Masumi; Higa, Olga Zazuco

doi:10.1111/j.1525-1594.2008.00542.x

Cited by 19 publications

(16 citation statements)

References 2 publications

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“…Cytotoxicity test: Following the guidelines of ISD 10993-5 [14] , the cytotoxicity test was performed in culture microplates seeded with CHD cells and sample extracts diluted from 100 to 6.25% [15] . The microplates were incubated for 72 h at 37 °C in a humidified 5% CD 2 atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Investigating the correlation between some of the properties of plasma nitrided AISI 316L stainless steel

et al. 2013

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When AISI 316L stainless steels are submitted to the nitriding process at temperatures lower than 450 °C, a high nitrogen content expanded austenite phase is formed, which shows higher hardness and higher pitting corrosion resistance compared to the untreated material. As a result, this material becomes adequate for biomedical application. The conditions of the nitriding technique, such as gas mixture, pressure, time and temperature, play an important role in some properties of the modified layer, including: thickness, hardness and N concentration along the layer. This paper explores a set of six samples of AISI 316L, nitrided at different times and temperatures, whose properties show important differences. The aim of this research is to investigate the correlation between the nitrided layer thickness (in the range of 0.77 to 11 µm) with both X-ray patterns characteristics and hardness measurements, which used two distinct loads. The results of this study show that: whereas the 3.6 gf load was suitable to measure the real hardness for four of the nitrided layers showing thickness ≥ 2.9 µm, the 50 gf load measured a substrate contribution, probably even for the highest thickness, 11 µm. Moreover, analyzing different reflections of the X-ray patterns showed evidence of the clear consistency between the X-Ray depths and the nitrided layer thicknesses: if the layer thickness is lower than the penetration depth of X-rays, two phases (austenite and expanded substrate) are present. If the layer thickness is higher, only the austenite is observed. Finally, concerning the citotoxicity property, all the samples, nitrided or not, were approved in the test for biocompatibility, indicating their potential use for biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Investigating the correlation between some of the properties of plasma nitrided AISI 316L stainless steel

et al. 2013

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“…Chinese hamster ovary cells (CHO-K1 lineage, ATCC number CCL-61) were maintained at 37°C in 5% CO 2 and 97% humidity in RPMI 1640 tissue culture medium (Gibco, USA), supplemented with 10% (v/v) fetal bovine serum (FBS -Gibco), (12). For subculturing and experiments, the cells were harvested using 0.05% (w/v) trypsin and 0.02% (w/v) ethylene diamine tetracetic acid (EDTA) in a saline phosphate-buffered solution, pH 7.4.…”

Section: Cell Line and Culture Conditionsmentioning

confidence: 99%

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

Torre¹,

Albuquerque²,

Farrapo³

et al. 2011

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/ plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

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“…The differential response in accordance with the cell type was observed in several studies, Ridolfi et al observed a higher survival in 3T3 than for HaCaT depending of the solid lipid nanoparticles employed, while Benavides et al observed higher cytotoxicity in 3T3 than for HaCaT in the phototoxicity of a surfactant probably related to variation in the ability to deal with oxidative stress. Rodas et al also observed a dose‐dependent cytotoxicity of the glycerol used to preserve bovine pericardium.…”

Section: Resultsmentioning

confidence: 95%

Natural latex‐glycerol dressing to reduce nipple pain and healing the skin in breastfeeding women

Barros

Santos

Miranda

et al. 2019

Skin Research and Technology

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Background Nipple pain is the second most common reason for early weaning, exceeded only by the insufficient milk supply. Nipple fissures can bring other problems, acting also as a portal for bacteria and leading to mastitis. This work proposes the breast protector composite development using materials with tissue repair and moisturizing properties, aligned with a low‐cost procedure, aiming not only to relieve pain, but also to heal the nipple fissures caused by breastfeeding. Materials and methods For the dressings, production was used Natural Latex extracted from the rubber tree and glycerol. The Samples were evaluated chemically and physically by the techniques of Scanning Electron Microscopy, Fourier transform infrared spectroscopy, mechanical traction, and contact angle. The samples were also biologically evaluated by the hemolytic and cytotoxic activity assays. Results From the physical‐chemical assays, the matrix with glycerol has high pore density; the natural latex and glycerol do not covalently interact, indicating that the glycerol can be released; the glycerol addition makes the matrix more elastic but fragile, and increase the wettability. From the biological assays, both materials showed no hemolytic effects; and the cytotoxicity results showed that glycerol did not present cytotoxicity in the fibroblasts, but show a dose‐dependent influence in the keratinocytes. Conclusion The material developed for application in breast fissures has mechanical properties similar to those found for materials for dermal applications, present high wettability and pore density. Furthermore, the material showed no cytolytic activity and the tests with skin cell cultures demonstrated the biocompatibility.

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Cytotoxicity and Genotoxicity of Bovine Pericardium Preserved in Glycerol

Cited by 19 publications

References 2 publications

Investigating the correlation between some of the properties of plasma nitrided AISI 316L stainless steel

Investigating the correlation between some of the properties of plasma nitrided AISI 316L stainless steel

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

Natural latex‐glycerol dressing to reduce nipple pain and healing the skin in breastfeeding women

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