Cytotoxicity and Genotoxicity of a New Intracanal Medicament, 2-hydroxyisocaproic Acid–An In Vitro Study

Selis, David; Pande, Yashika; Smoczer, Cristine; Wheater, Michelle; Alhabeil, Jamal A.; Paurazas, Susan; Askar, Mazin

doi:10.1016/j.joen.2019.01.012

Cited by 18 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In certain cases the tooth might require nonsurgical management if the damage is extreme (Fernandes and Ataide, 2010). Analysing the benefits and risks associated with the use of these medicaments is of extreme importance most specifically in regenerative endodontic procedures because the residual stem cells may get damaged (Selis et al, 2019). : Bar graph denotes association between year of study of the participants and number of dental students who are aware of the cytotoxicity of the medicament they use.…”

Section: Resultsmentioning

confidence: 99%

“…Intracanal medicaments are made of various compounds like the phenolic compounds which includes crestatin, camphorated monochlorophenol and aldehydes that includes formocresol and glutaraldehyde, as well as other materials such as calcium hydroxide and some antibiotics (Johnson and noblett, 2009) A successful outcome of root canal therapy relies on effective disinfection of the root canal system, including the use of intracanal medicaments, which vary in their bactericidal and cytotoxic properties (Selis et al, 2019). Intracanal medications need to be biocompatible as they come in direct contact with periapical tissues during endodontic treatment.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Knowledge, Attitude and Practice on Cytotoxicity of Intracanal Medicaments Among Dental Students

Mariona¹

2020

Biosci. Biotech. Res. Comm

View full text Add to dashboard Cite

The main objective of a root canal treatment is to get rid of bacteria inside the root canal treatment. Placement of an intracanal medicament is an integral step in the treatment procedure. Cytotoxicity is a characteristic of being toxic to cells.In some cases if the intracanal medicament extrudes beyond the root canal, it is seen to cause damage to the cells of the periapical tissues that subsequently lead to the damage or inflammation of the periapical region. This study aims at assessing the knowledge, attitude and practice among dental undergraduates on the cytotoxic effects of intracanal medicaments. A Knowledge, Attitude and Practice based survey was conducted in January 2020 among dental students (Third years, Final years, Interns). The questionnaire consisted of 10 Knowledge, awareness and practice based questions and were equally distributed among Third years, Final years, Interns. The total sample size was 150 dental students. The data collected was entered in an Excel sheet and subjected to statistical analysis using SPSS version 20. From the responses obtained it was seen that 33.33% of the interns had a knowledge of intracanal medicaments being used whereas only 26.67% of final years and 10% of third years had a knowledge. 33.33% of the interns had used an intracanal medicament on the patient and only 26.67% of final years and 4.67% of third years had used a medicament. 21.33% of the final years said that they use an intracanal medicament for all patients whereas 20.67% of interns and 4% of third years have given a response of yes. 30.67% of interns, 22.67% of final years, 4.67% of third years knew the mechanism of action of the medicament. 33.33% of interns, 33.33% of final years and 8.67% of third years were aware of the term cytotoxicity. Chi square test shows p<0.05, significant. It was seen that Knowledge, awareness and practice on the cytotoxicity of intracanal medicaments was higher among interns, followed by the final years and then third years

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Knowledge, Attitude and Practice on Cytotoxicity of Intracanal Medicaments Among Dental Students

Mariona¹

2020

Biosci. Biotech. Res. Comm

View full text Add to dashboard Cite

show abstract

“…Moreover, both novel antimicrobial agents are low cytotoxicity. HICA was not cytotoxic to human periodontal ligament fibroblasts at concentrations <10 mg/mL, it was less cytotoxic than calcium hydroxide at standard concentrations of 1 mg/mL (Selis et al, 2019). Alpha‐mangostin was biocompatible when in contact with human gingival fibroblast (Kaomongkolgit et al, 2009) and human PDL cells (Kaomongkolgit et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of D,L‐2‐hydroxyisocaproic acid (HICA) and alpha‐mangostin against endodontopathogenic microorganisms in a multispecies bacterial–fungal biofilm in an ex vivo tooth model

et al. 2021

View full text Add to dashboard Cite

Aim To develop a defined multispecies root canal biofilm model ex vivo, and to perform viable compositional analysis following D,L‐2‐hydroxyisocaproic acid (HICA), alpha‐mangostin, Calcicur®, and Odontopaste® exposure. Methodology Time‐kill assays were conducted in vitro using HICA, alpha‐mangostin, Calcicur®, Odontopaste®, and saline solution on the planktonic cultures of C. albicans, E. faecalis, L. rhamnosus, and S. gordonii. Human root dentine blocks were prepared (n = 100) ex vivo, and multispecies suspensions containing each of 1.5 × 108 CFU/mL C. albicans, E. faecalis, L. rhamnosus, and S. gordonii in brain heart infusion (BHI) were incubated within the root canals for 21 days. Canals (n = 20/group) were then exposed to medicaments for 7 days. Samples taken from the inner (first 0.1 mm) and deeper (second 0.1 mm) dentine by drilling with Ash Steel Burs No. 5 and No. 6, and residual roots were cultured in broth for 24 h. Cell growth was detected by spectrophotometry and confirmed by culture on agar. The other set of inner dentine, deeper dentine, and residual root samples were sonicated, and then exposed with 50 μM PMA before DNA was extracted using the QIAamp DNA mini kit. Real‐time quantitative PCR was performed to determine the biofilm composition as well as the number of live and total cells remaining in the biofilm following each treatment. The OD data were analysed with Kruskal–Wallis and Friedman with Wilcoxon signed‐rank test between and within groups, respectively, agar culture and qPCR data with Pearson chi‐square with Mann–Whitney and Cochran with McNemar tests, respectively (p < .0001). Results Time‐kill assays revealed that HICA and Calcicur® killed all planktonic organisms within 24 h, whilst alpha‐mangostin killed the organisms within 72 h. However, Odontopaste® was a slow‐killing agent: 10 cells of planktonic organisms survived after exposure to the agent for 7 days. The ex vivo tooth model demonstrated that HICA and alpha‐mangostin significantly inhibited the cell growth in all sampling depths (p < .0001). All species‐specific data revealed the effectiveness of each medicament on the biofilm composition. Conclusions D,L‐2‐hydroxyisocaproic acid and alpha‐mangostin had antimicrobial activity against multispecies bacterial–fungal biofilms.

show abstract

“…The variability of HICA levels in commercial yogurts [ 4 ] may reflect the diversity of activities of hydroxyisocaproate dehydrogenase enzymes in the diverse Lactobacillus strains used in fermentation [ 3 ]. HICA cannot be degraded by many bacterial species, and its production may thus represent a survival strategy for Lactobacillus , because HICA displays antibacterial activity [ 5 ]. In addition to its impact on bacteria, HICA is generally considered to act as an anticatabolic agent in mammalian tissues [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube

et al. 2021

View full text Add to dashboard Cite

There is ongoing debate as to whether or not α-hydroxyisocaproic acid (HICA) positively regulates skeletal muscle protein synthesis resulting in the gain or maintenance of skeletal muscle. We investigated the effects of HICA on mouse C2C12 myotubes under normal conditions and during cachexia induced by co-exposure to TNFα and IFNγ. The phosphorylation of AMPK or ERK1/2 was significantly altered 30 min after HICA treatment under normal conditions. The basal protein synthesis rates measured by a deuterium-labeling method were significantly lowered by the HICA treatment under normal and cachexic conditions. Conversely, myotube atrophy induced by TNFα/IFNγ co-exposure was significantly improved by the HICA pretreatment, and this improvement was accompanied by the inhibition of iNOS expression and IL-6 production. Moreover, HICA also suppressed the TNFα/IFNγ co-exposure-induced secretion of 3-methylhistidine. These results demonstrated that HICA decreases basal protein synthesis under normal or cachexic conditions; however, HICA might attenuate skeletal muscle atrophy via maintaining a low level of protein degradation under cachexic conditions.

show abstract

Cytotoxicity and Genotoxicity of a New Intracanal Medicament, 2-hydroxyisocaproic Acid–An In Vitro Study

Cited by 18 publications

References 19 publications

Knowledge, Attitude and Practice on Cytotoxicity of Intracanal Medicaments Among Dental Students

Knowledge, Attitude and Practice on Cytotoxicity of Intracanal Medicaments Among Dental Students

Effectiveness of D,L‐2‐hydroxyisocaproic acid (HICA) and alpha‐mangostin against endodontopathogenic microorganisms in a multispecies bacterial–fungal biofilm in an ex vivo tooth model

α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube

Contact Info

Product

Resources

About