Cytotoxicity and apoptosis induction in human breast adenocarcinoma MCF-7 cells by (+)-cyanidan-3-ol

Monga, Jitender; Pandit, Saurabh; Chauhan, Chetan Singh; Sharma, Manu

doi:10.1016/j.etp.2013.04.005

Cited by 23 publications

(16 citation statements)

References 30 publications

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“…Reduction in growth of cancer cells after treatment with various plant extracts was also observed by other researchers, indicating alterations in growth kinetics of cancer cells. 17 The growth rate of cell lines which are adherent in nature is highly dependent on the environment in which they grow. Various factors, which may be either chemical or physical in nature, will impact the growth.…”

Section: Discussionmentioning

confidence: 99%

“…The experimental protocol consisted of seeding 10 5 cells per well in a 12-well plate and treatment with 30C and MT, for a period of 48 h. Following this, changes in cell growth, which corresponded to the number of attached cells, was observed and the phase contrast images were taken at 100Â magnification using Nikon eclipse Ti microscope. 17…”

Section: Assessment Of Growth Kineticsmentioning

confidence: 99%

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DNA fragmentation and cell cycle arrest: a hallmark of apoptosis induced by Ruta graveolens in human colon cancer cells

Arora

Tandon

2015

Homeopathy

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In the present study, we investigated the anti-cancer effect of various potencies of Ruta graveolens (Ruta) on COLO-205 cell line, as evidenced by cytotoxicity, migration, clonogenecity, morphological and biochemical changes and modification in the levels of genes associated with apoptosis and cell cycle. On treatment of COLO-205 cells maximal effects were seen with mother tincture (MT) and 30C potencies, wherein decrease in cell viability along with reduced clonogenecity and migration capabilities were noted. In addition morphological and biochemical alterations such as nuclear changes (fragmented nuclei with condensed chromatin) and DNA ladder-like pattern (increased amount of fragmented DNA) in COLO-205 cells indicating apoptotic related cell death were seen. The expression of apoptosis and cell-cycle related regulatory genes assessed by reverse transcriptase-PCR revealed an up-regulation of caspase 9, caspase-3, Bax, p21 and p27 expression and down-regulation of Bcl-2 expression in treated cells. The mode of cell death was suggestive of intrinsic apoptotic pathway along with cell cycle arrest at the G2/M of the cell cycle. Our findings indicate that phytochemicals present in Ruta showed potential for natural therapeutic product development for colon carcinoma.

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Section: Discussionmentioning

confidence: 99%

Section: Assessment Of Growth Kineticsmentioning

confidence: 99%

DNA fragmentation and cell cycle arrest: a hallmark of apoptosis induced by Ruta graveolens in human colon cancer cells

Arora

Tandon

2015

Homeopathy

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“…The most identifiable morphological features of apoptosis were observed by inverted light microscopy in the RFRA treated cells. The treated cells appeared like cells undergoing apoptosis with prominent features such as detaching from the culture plate, cytoplasmic condensation, cell shrinkage and condensation and aggregation of the nuclear chromatin, and loss of contact with neighbouring cells [ 25 ]. However the untreated cells appeared normal and were confluent.…”

Section: Resultsmentioning

confidence: 99%

In VitroAntiproliferative Effect of the Acetone Extract ofRubus fairholmianusGard. Root on Human Colorectal Cancer Cells

George

Tynga

Abrahamse

2015

BioMed Research International

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Plants and plant derived products exert chemopreventive effects on various cancer cell lines by the induction of cell death mechanisms. The effects of root acetone extract of Rubus fairholmianus (RFRA) on the proliferation of human colorectal cancer (Caco-2) cells have been investigated in this study. The extract led to a dose dependent decrease in both viability and proliferation and increased cytotoxicity using trypan blue exclusion, adenosine 5′-triphosphate (ATP), and lactate dehydrogenase (LDH) assay. The morphological features of the treated cells were supportive for the antiproliferative activity. The Annexin V/propidium iodide staining indicated that R. fairholmianus induced toxic effects in Caco-2 cells and the percentages of the early and late apoptotic population significantly increased when compared with control cells. Also we studied the apoptosis inducing ability of the extract by analysing caspase 3/7 activity and the induction of cell death via the effector caspases was confirmed; the activity increased in treated cells compared with control. Thus the present findings highlight that the R. fairholmianus root acetone extract exhibits antiproliferative activity on Caco-2 cells by the induction of apoptosis via caspase dependent pathway.

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“…1). 20 These results indicated the potential of (+)-catechin as a cancer chemopreventive agent. 15 In our previous research, we found that a (+)-catechin rich aqueous extract of Acacia catechu shows chemopreventive effects against skin, 16 liver, 17 and breast cancers.…”

Section: Introductionmentioning

confidence: 81%

Topical (+)-catechin emulsified gel prevents DMBA/TPA-induced squamous cell carcinoma of the skin by modulating antioxidants and inflammatory biomarkers in BALB/c mice

et al. 2014

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An emulsified gel of (+)-catechin was developed and evaluated topically against 7,12-dimethylbenz(a)anthracene-induced and 12-O-tetradecanoylphorbol-13-acetate-promoted (DMBA-induced and TPA-promoted) squamous cell carcinoma of the skin in BALB/c mice. The biological evaluation outcome indicated that the (+)-catechin emulsified gel increased the activity of oxidative stress biomarkers glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx), whereas it decreased the level of malondialdehyde (MDA). The mechanistic study showed that genes implicated in the inflammation and cancer, such as cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB), and inducible nitric-oxide synthase (iNOS), were down-regulated by (+)-catechin emulsified gel while inhibiting an inflammatory mediator prostaglandin E2 (PGE2). The (+)-catechin emulsified gel further suppressed the activity of pro-inflammatory cytokines, viz. tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). The in vitro permeation study revealed that release of (+)-catechin from an emulsified gel base reached a steady state after 6 h, while pH of the entire emulsified gel was found to be between 6.2 and 6.5 that falls well within the normal pH range of the skin.

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Cytotoxicity and apoptosis induction in human breast adenocarcinoma MCF-7 cells by (+)-cyanidan-3-ol

Cited by 23 publications

References 30 publications

DNA fragmentation and cell cycle arrest: a hallmark of apoptosis induced by Ruta graveolens in human colon cancer cells

DNA fragmentation and cell cycle arrest: a hallmark of apoptosis induced by Ruta graveolens in human colon cancer cells

In VitroAntiproliferative Effect of the Acetone Extract ofRubus fairholmianusGard. Root on Human Colorectal Cancer Cells

Topical (+)-catechin emulsified gel prevents DMBA/TPA-induced squamous cell carcinoma of the skin by modulating antioxidants and inflammatory biomarkers in BALB/c mice

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