Cytotoxicity and antimicrobial efficiency of ZrO2 nanoparticles reinforced 3D printed resins

Aati, Sultan; Shrestha, Barsha; Fawzy, A.

doi:10.1016/j.dental.2022.06.030

Cited by 34 publications

(21 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hydrophilicity/hydrophobicity significantly influence the microorganism adherence and accumulation on materials surface ( Hamid et al., 2021 ). The hydrophobic interaction occurs between the microbial surface and PMMA resin, leading the microbial cells to overcome the initial electrostatic repulsive forces between them ( Krasowska and Sigler, 2014 ; Aati et al., 2022 ), thereby enhancing microbial biofilm adherence over the substrates. Another plausible explanation could be that hydrophobic surfaces favors proteins accumulation which provide specific binding sites for microbiomes and thus accelerating and facilitating their adhesion as well ( An and Friedman, 1998 ).…”

Section: Discussionmentioning

confidence: 99%

“…Candida-associated denture stomatitis has been found in up to 60% of denture wearers, being the most common oral mucosal issue related with denture base. To overcome such undesirable deficiency, considerable research about PMMA denture base resin modification have been studied, including incorporating nanoparticles, fibers or other fillers, utilizing copolymers to polymerize with PMMA resin matrix and curing in different conditions, etc ( Gad et al., 2020 ; Karatepe and Ozdemir, 2020 ; Aati et al., 2022 ). The modified methods of PMMA based denture resin with monomer additives have been reported before ( Kanie et al., 2004 ; Elzahar et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

Song

Zhao

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

ObjectivesConsidering the high incidence rates of denture stomatitis, research that providing dental biomaterials with antifungal property are essential for clinical dentistry. The objectives of the present study were to investigate the effect of zinc dimethacrylate (ZDMA) modification on the antifungal and cytotoxic properties, as well as the variance in surface characteristics and other physicochemical properties of polymethyl methacrylate (PMMA) denture base resin.MethodsPMMA with various mass fraction of ZDMA (1 wt%, 2.5 wt% and 5 wt%) were prepared for experimental groups, and unmodified PMMA for the control. Fourier-transform infrared spectroscopy (FTIR) was applied for characterization. Thermogravimetric analysis, atomic force microscopy and water contact angle were performed to investigate the thermal stability and surface characteristics (n=5). Antifungal capacities and cytocompatibility were evaluated with Candida albicans (C. albicans) and human oral fibroblasts (HGFs), respectively. Colony-forming unit counting, crystal violet assay, live/dead biofilm staining and scanning electron microscopy observation were performed to assess antifungal effects, and the detection of intracellular reactive oxygen species production was applied to explore the possible antimicrobial mechanism. Finally, the cytotoxicity of ZDMA modified PMMA resin was evaluated by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and live/dead double staining.ResultsThe FTIR analyses confirmed some variation in chemical bonding and physical blend of the composites. Incorporation of ZDMA significantly enhanced the thermal stability and hydrophilicity compared with unmodified PMMA (p < 0.05). The surface roughness increased with the addition of ZDMA while remained below the suggested threshold (≤ 0.2 µm). The antifungal activity significantly improved with ZDMA incorporation, and cytocompatibility assays indicated no obvious cytotoxicity on HGFs.ConclusionsIn the present study, the ZDMA mass fraction up to 5 wt% in PMMA performed better thermal stability, and an increase in surface roughness and hydrophilicity without enhancing microbial adhesion. Moreover, the ZDMA modified PMMA showed effective antifungal activity without inducing any cellular side effects.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

Song

Zhao

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Aati, Shrestha and Fawzy [ 99 ] synthesized zirconium dioxide nanoparticles (ZrO 2 NPs) and studied their antimicrobial and cytotoxic effects in a modified three-dimensional printed resin, which is routinely used in dentistry. The diameters of ZrO 2 NPs ranged from 20 to 40 nm before and after functionalization.…”

Section: Metal Nanoparticlesmentioning

confidence: 99%

Metal Nanoparticles to Combat Candida albicans Infections: An Update

et al. 2023

View full text Add to dashboard Cite

Candidiasis is an opportunistic mycosis with high annual incidence worldwide. In these infections, Candida albicans is the chief pathogen owing to its multiple virulence factors. C. albicans infections are usually treated with azoles, polyenes and echinocandins. However, these antifungals may have limitations regarding toxicity, relapse of infections, high cost, and emergence of antifungal resistance. Thus, the development of nanocarrier systems, such as metal nanoparticles, has been widely investigated. Metal nanoparticles are particulate dispersions or solid particles 10–100 nm in size, with unique physical and chemical properties that make them useful in biomedical applications. In this review, we focus on the activity of silver, gold, and iron nanoparticles against C. albicans. We discuss the use of metal nanoparticles as delivery vehicles for antifungal drugs or natural compounds to increase their biocompatibility and effectiveness. Promisingly, most of these nanoparticles exhibit potential antifungal activity through multi-target mechanisms in C. albicans cells and biofilms, which can minimize the emergence of antifungal resistance. The cytotoxicity of metal nanoparticles is a concern, and adjustments in synthesis approaches or coating techniques have been addressed to overcome these limitations, with great emphasis on green synthesis.

show abstract

“…PMMA can be also used as a biomedical material, such as an important component of bone cement, and acrylic denture base because of its biocompatibility with human and animal bodies. [1][2][3][4] Zirconia is a wellknown biomedical ceramic material that has been used as bone tissue because of its excellent bioactivity. [5][6][7] Recently, polymer nanocomposites have been the hot topic in material science because of some superior properties to conventional polymer composites.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of PMMA/ZrO₂ nanocomposite 3D printing filaments

Linh,

Huy,

Dung

et al. 2023

Vietnam Journal of Chemistry

View full text Add to dashboard Cite

The 3D printing PMMA/ZrO2 filaments with a diameter of 1.75 ± 0.05 mm were fabricated by using a single‐screw extruder at temperatures of four heating zones of 190, 200, 210, and 210°C. Testing samples were prepared from the extruded filaments by using a fusion deposition modeling 3D printer and a Haake MiniJet piston injection molding machine. Fourier transform infrared spectroscopy, mechanical properties, field emission scanning electron microscopy (FESEM), differential scanning calorimetry, and thermogravimetric analysis of the filaments were investigated. It was suggested the formation of physical and molecular interactions between surface hydroxyl groups of ZrO2 nanoparticles and C‐O‐C groups of PMMA in the PMMA/ZrO2 filaments. FESEM images of the filaments indicated the good dispersion of ZrO2 nanoparticles in the PMMA matrix, nevertheless, there was still the formation of clusters of ZrO2 nanoparticles in the nanocomposite filaments. The incorporation of nano ZrO2 increased the thermal properties, bending, and tensile moduli of PMMA. The mechanical properties of the 3D‐printed samples were lower than those of the molded samples. However, the bending strength of the 3D‐printed samples was much higher than 50 MPa, bending modulus was much higher than 1800 MPa, as required for acrylic bone cement (in ISO 5833:2002).

show abstract

Cytotoxicity and antimicrobial efficiency of ZrO2 nanoparticles reinforced 3D printed resins

Cited by 34 publications

References 36 publications

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

Metal Nanoparticles to Combat Candida albicans Infections: An Update

Fabrication and characterization of PMMA/ZrO₂ nanocomposite 3D printing filaments

Contact Info

Product

Resources

About

Cytotoxicity and antimicrobial efficiency of ZrO2 nanoparticles reinforced 3D printed resins

Cited by 34 publications

References 36 publications

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

Metal Nanoparticles to Combat Candida albicans Infections: An Update

Fabrication and characterization of PMMA/ZrO2 nanocomposite 3D printing filaments

Contact Info

Product

Resources

About

Fabrication and characterization of PMMA/ZrO₂ nanocomposite 3D printing filaments