Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA

Abstract

Purpose. To compare the biocompatibility and antimicrobial effectiveness of the new Fast-Set MTA (FS-MTA) with ProRoot MTA (RS-MTA). Methods. The agar overlay method with neutral red dye was used. L929 mouse fibroblast cells were cultured. The liquid and oil extracts and solid test material were placed on the agar overlay, four samples for each material. Phenol was used as the positive control and cottonseed oil and MEM extracts were used as negative controls. Cytotoxicity was examined by measuring the zones of decolorization and evaluating cell lysis under an inverted microscope using the established criteria after 24 and 48 hours. The antimicrobial test was performed using the Kirby-Bauer disk-diffusion method against S. mutans, E. faecalis, F. nucleatum, P. gingivalis, and P. intermedia. The size of the zone of inhibition was measured in millimeters. Results. There was no zone of decolorization seen under or around the test materials for FS-MTA and RS-MTA at 24 and 48 hours. The antimicrobial test demonstrated no inhibitory effect of FS-MTA or RS-MTA on any bacterial species after 24 and 48 hours. Conclusions. There was no cytotoxicity or bacterial inhibition observed by the new Fast-Set MTA when compared to the ProRoot MTA after setting.

Novel anti-biofouling bioactive calcium silicate-based cement containing 2-methacryloyloxyethyl phosphorylcholine

“…However, the addition of MPC into CSC caused a significant increase in setting time to nearly 1 h for 10% MPC. A long setting time is a clinical disadvantage as it would increase patient chair-time, which may not be ideal for restless or uncooperative patients (e.g., pediatric patients) and would consequently increase the risk of contamination [23,26].…”

“…The disc diffusion test, adapted from a previous study [23], was used to measure bactericidal properties. The same bacterial culture of Enterococcus faecalis (ATCC 29212) was used for this study.…”

“…A filter-paper disc with same diameter as the disc-shaped sample was placed on the surface of the agar plate soaked with 20 μL of 5.25% sodium hypochlorite (NaOCl) as a positive control. The choice of the positive control was based on a previous study that used NaOCl as an effective irrigation solution to effectively dissolve organic matter and kill microbes at concentrations of higher than 1% to 2% [23]. The plates were incubated for 24 h at 37°C and the inhibition zones around each sample were measured with Vernier calipers (Mitutoyo, Kawasaki, Japan).…”

Comparative Evaluation of Biological Performance, Biosecurity, and Availability of Cellulose-Based Absorbable Hemostats

“…The cytotoxicity was moderate if the reactivity zone extended specimen up to 1.0 cm, and it was severely cytotoxic when the extending of reactivity zone was further than 1.0 cm beyond specimen. 40…”

Successful Use of MTA Fillapex as a Sealant for Feline Root Canal Therapy of 50 Canines in 37 Cats

“…15,27,32,40,41 Studies have shown that MTA is biocompatible, 15,19,27,29,32,34,38,[42][43][44][45][46][47][48] has bactericidal effects, 29,43,44 is noncytotoxic, 45 radiopaque, 30,43 bactericidal, 45 can create an impervious durable seal, 15,25,29,30,38,[44][45][46][49][50][51] is dimensionally stable, 15 has good marginal adaption, 51 and can promote osteogenesis, odontogenesis, cementosis, 15,16,29,32,38,44,[50][51][52][53][54] and the regeneration of periradicular tissues.…”