Cytotoxicity and antimicrobial activities of secondary metabolites produced by endophytic fungi isolated from Gongronema latifolia and Loranthus micrantus

Isolation, characterization, and screening of secondary metabolites from endophytic fungi isolated from Nigerian Piliostigma thonningii for antimicrobial activities

“…The isolation of endophytic fungi from the plant leaves was carried out according to the procedures outlined by Eze et al 15 and Abba et al 16 . The plant leaves were washed thoroughly under running tap water to remove the debris adhered and finally washed with double distilled deionized water.…”

“…The rich plant biodiversity of Nigeria has been reported to contain diverse endophytic microbial communities which present the opportunity for the discovery of biologically important compounds of pharmaceutical and industrial importance 4,[15][16][17][18][19][20][21][22][23][24][25][26] . There has been no report of the isolation or screening of secondary metabolites of any endophyte associated with P. thonningii hence our study seeks to isolate and investigate the secondary metabolites of an endophytic fungi isolated from leaves P. thonningii growing in Sokoto State, North-Western Nigeria.…”

Phenolic Compounds from Endophytic Pseudofusicoccum sp. Isolated from Annona muricata

“…The isolation of an endophytic fungus from the plant leaves was carried out using a previously described method. 26 The plant leaves were washed in running tap water and then cut into small fragments of about 1 cm 2 . The leaf fragments were surface-sterilized by immersion in 2% sodium hypochlorite solution for 2 min, 70% ethanol for nearly 2 min, and then rinsed in sterile water for 5 min.…”

“…The antimicrobial assay of the endophytic fungal crude extract (1 mg/mL) was performed using the agar well diffusion assay method as described by Abba et al 26 The antibacterial activity of the fungal extract was tested against laboratory strains of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, while Aspergillus niger and Candida albicans were used for the antifungal assay. Ciprofloxacin (5 μg/mL) and miconazole (50 µg/mL) served as positive control for the antibacterial and antifungal assays respectively, while DMSO was used as the negative control.…”

“…24 Investigations of the endophytic fungal populations of some Nigerian medicinal plants have revealed the enormous potentials possessed by these organisms as sources of compounds of pharmaceutical and industrial importance. [24][25][26][27][28][29][30][31] There is, therefore, the need to further explore these plants for endophytes producing biologically important molecules. Hence, our study seeks to investigate the secondary metabolites of an endophytic fungus isolated from leaves A. muricata growing in Anambra State, South-East Nigeria.…”