Cytotoxicity analysis of pre- and post-hurricane harvey soil samples collected from greater houston bayous

Keita, Djene; Shishodia, Shishir; Sridhar, B. B. Maruthi

doi:10.1016/j.ecoenv.2021.112600

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…C644‐0303 efficiently inhibited luciferase activity of TCF/LEF (IC 50 , 4.20 μmol/L) but not negative control FOPFlash (Figures 1D and A). Consistently, C644‐0303 potently impeded the cell growth of HCT‐116 (IC 50 , 17.69 μmol/L) but not a human normal colon epithelial cell line CCD‐841CoN 23 (IC 50 , 53.33 μmol/L) (Figures 1E and B) while the IC 50 of known Wnt signaling inhibitor IWR‐1 24 is 60.77 μmol/L for HCT‐116 and more than 100 μmol/L for CCD‐841CoN (Figure C,D). These data suggested that C644‐0303 could target the Wnt signaling pathway in mutant CRC with strong inhibitory potency, while its potential toxicity to normal colon cells could be further optimized.…”

Section: Resultsmentioning

confidence: 76%

C644‐0303, a small‐molecule inhibitor of the Wnt/β‐catenin pathway, suppresses colorectal cancer growth

Yan

Zhang

et al. 2021

Cancer Science

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The Wnt/β‐catenin signaling pathway plays an important role in tissue homeostasis, and its malignant activation is closely related to the occurrence and development of many cancers, especially colorectal cancer with adenomatous polyposis coli (APC) and CTNNB1 mutations. By applying a TCF/lymphoid‐enhancing factor (LEF) luciferase reporter system, the high‐throughput screening of 18 840 small‐molecule compounds was performed. A novel scaffold compound, C644‐0303, was identified as a Wnt/β‐catenin signaling inhibitor and exhibited antitumor efficacy. It inhibited both constitutive and ligand activated Wnt signals and its downstream gene expression. Functional studies showed that C644‐0303 causes cell cycle arrest, induces apoptosis, and inhibits cancer cell migration. Moreover, transcription factor array indicated that C644‐0303 could suppress various tumor‐promoting transcription factor activities in addition to Wnt/β‐catenin. Finally, C644‐0303 suppressed tumor spheroidization in a 3‐dimensional cell culture model and inhibited xenograft tumor growth in mice. In conclusion, we report a novel structural small molecular inhibitor targeting the Wnt/β‐catenin signaling pathway that has therapeutic potential for colorectal cancer treatment.

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Section: Resultsmentioning

confidence: 76%

C644‐0303, a small‐molecule inhibitor of the Wnt/β‐catenin pathway, suppresses colorectal cancer growth

Yan

Zhang

et al. 2021

Cancer Science

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Risk Assessment of Displaced Sediment by an Extreme Event Cyclone in a Peri-Urban Zone Using Bioassays and Analytical Chemistry

Tremblay,

Nakajima,

Endo

et al. 2024

Toxics

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Hawke’s Bay in New Zealand was impacted by Cyclone Gabrielle in 2023, experiencing intense weather conditions and rainfall. Rivers and streams surged beyond their banks, displacing large amounts of sediment. The sewage treatment plant and industries in the Waitangi catchment, south of the city of Napier, were heavily impacted, making them potential sources of contaminants. The aim of this study was to investigate the risk of displaced sediments deposited south of Napier City, using bioassays and chemical analysis methods. Sediment samples were collected across a gradient between the coastline and the Waitangi Stream. The toxicity of chemically extracted or elutriate samples was assessed by Microtox®, mussel embryo–larval development, and aryl hydrocarbon and constitutive androstane receptor yeast two-hybrid assays. Targeted chemical analysis and automated identification and quantification system (AIQS-GC) methods were used to identify contaminants. The elutriates showed low toxicity and the yeast assays showed levels of activity like those previously reported. Chemical methods confirmed historical contamination by DDT and its metabolites DDE and DDD, as well as by plant sterols. Overall, the toxicity and chemicals detected are what would be expected from a typical agricultural soil. The risk posed by the displaced sediment in the Waitangi catchment can be considered low. Combining chemical and bioanalytical methods was an effective approach to investigate the potential risks of post-disaster contamination.

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Cytotoxicity analysis of pre- and post-hurricane harvey soil samples collected from greater houston bayous

Cited by 2 publications

References 29 publications

C644‐0303, a small‐molecule inhibitor of the Wnt/β‐catenin pathway, suppresses colorectal cancer growth

C644‐0303, a small‐molecule inhibitor of the Wnt/β‐catenin pathway, suppresses colorectal cancer growth

Risk Assessment of Displaced Sediment by an Extreme Event Cyclone in a Peri-Urban Zone Using Bioassays and Analytical Chemistry

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