Cytotoxic triterpenoid glycosides (saikosaponins) from the roots of Bupleurum chinense

Li, Dan-Qi; Wu, Jie; Liu, Li‐Yin; Wu, Ying‐Ying; Li, Lingzhi; Huang, Xiao‐Xiao; Liu, Qingbo; Yang, Jingyu; Song, Shao‐Jiang; Wu, Chunfu

doi:10.1016/j.bmcl.2015.07.053

Cited by 44 publications

(24 citation statements)

References 46 publications

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“…Although dozens of different SS monomer compounds have been detected in the roots of Bupleurum, little investigation has been conducted on the biosynthesis and regulation of different SS monomers. This is especially true for minor compounds and their mutual transformation in a given Bupleurum species (Li et al 2015). In this study, we compared the content of SS a, SS c and SS d, the three major SS monomers in Bupleurum, with reference standard compounds.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of BcbZIP134 negatively regulates the biosynthesis of saikosaponins

et al. 2019

Plant Cell Tiss Organ Cult

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Chaihu, the dried roots of several Bupleurum species, are widely used as herbal drugs. Triterpene saponins, as saikosaponins (SSs) in Chaihu, exhibit a wide range of pharmacological actions, including significant anti-inflammatory, immunomodulatory, hepatoprotective, antioxidant, anti-tumor and antiviral activities. To explore the regulatory biosynthesis of SS in Bupleurum chinense roots, we screened putative transcription factor (TF) genes from transcriptome data. Here, we report one of the bZIP TF genes, named BcbZIP134, which belongs to bZIP G subfamily. The expression of BcbZIP134 was elevated in MeJA-treated adventitious roots of B. chinense. No obvious differential expression of BcbZIP134 was detected in tissues (root, stem, leaf, flower and fruit) of B. chinense. Overexpressing BcbZIP134 in B. chinense hairy roots revealed that the content of the main SS monomers a, c and d were decreased compared to control hairy roots. Furthermore, the contents of a few other minor SS monomers were also affected when compared to the controls. The results indicate that BcbZIP134 may play a negative regulatory role in SS biosynthesis. Key MessageChaihu, the dried roots of several Bupleurum species, are successfully and widely used as herbal drugs. Triterpene saponins, as saikosaponins (SSs) in Chaihu, exhibit a wide range of pharmacological actions, including significant anti-inflammatory, immunomodulatory, hepatoprotective, antioxidant, anti-tumor and antiviral activities. To explore the regulatory biosynthesis of SS in B. chinense roots, we screened putative transcription factor (TF) genes from transcriptome data. Here, we report one of the bZIP TF genes, named BcbZIP134, which encodes a predicted 410 amino acid protein belonging to bZIP G subfamily. The expression of BcbZIP134 was elevated in MeJA-treated adventitious roots of B. chinense, and no obvious differential expression of BcbZIP134 was detected in tissues (root, stem, leaf, flower and fruit) of B. chinense. Overexpressing BcbZIP134 in B. chinense hairy roots revealed that the content of the main SS monomers a, c and d were decreased compared to control hairy roots. Furthermore, the contents of a few other minor SS monomers were also affected when compared to the controls.The results indicate that BcbZIP134 may play a negative regulatory role in SS biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Overexpression of BcbZIP134 negatively regulates the biosynthesis of saikosaponins

et al. 2019

Plant Cell Tiss Organ Cult

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“…Triterpenoid saponins are the main active components of Radix Bupleuri, which exhibit a broad spectrum of biological and pharmacological effects, including analgesic, immunomodulatory, hepatoprotective, immunomodulatory, anti-inflammatory, antitumor, and antiviral activities [3,[41][42][43]. Currently, approximately 35 saponins have been isolated from Radix Bupleuri (Figure 2) [44][45][46][47][48][49][50][51][52][53][54]. Among them, saikosaponins a, c, and d are the major bioactive constituents found in Radix Bupleuri; however a variety of minor saikosaponins have also been isolated [16].…”

Section: Triterpenoid Saponinsmentioning

confidence: 99%

Radix Bupleuri: A Review of Traditional Uses, Botany, Phytochemistry, Pharmacology, and Toxicology

Yang

Dong

Yin

et al. 2017

BioMed Research International

123

125

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Radix Bupleuri (Chaihu) has been used as a traditional medicine for more than 2000 years in China, Japan, Korea, and other Asian countries. Phytochemical studies demonstrated that this plant contains essential oils, triterpenoid saponins, polyacetylenes, flavonoids, lignans, fatty acids, and sterols. Crude extracts and pure compounds isolated from Radix Bupleuri exhibited various biological activities, such as anti-inflammatory, anticancer, antipyretic, antimicrobial, antiviral, hepatoprotective, neuroprotective, and immunomodulatory effects. However, Radix Bupleuri could also lead to hepatotoxicity, particularly in high doses and with long-term use. Pharmacokinetic studies have demonstrated that the major bioactive compounds (saikosaponins a, b2, c, and d) were absorbed rapidly in rats after oral administration of the extract of Radix Bupleuri. This review aims to comprehensively summarize the traditional uses, botany, phytochemistry, pharmacology, toxicology, and pharmacokinetics of Radix Bupleuri reported to date with an emphasis on its biological properties and mechanisms of action.

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“…belonging to Bupleurum genus (Umbelliferae), customarily called ‘Beichaihu’ in China, have been used for approximately 2000 years. As one of the most common ingredients in Chinese medicine prescription, it had the effect of treating fever, chronic hepatitis, influenza, kidney syndrome, menstrual disorders, and peptic ulcers . Besides, the aerial parts of Bupleurum chinense DC.…”

Section: Introductionmentioning

confidence: 99%

“…As one of the most common ingredients in Chinese medicine prescription, it had the effect of treating fever, chronic hepatitis, influenza, kidney syndrome, menstrual disorders, and peptic ulcers. [1] Besides, the aerial parts of Bupleurum chinense DC. have been used in folk medicine in some areas of southeast China and Spain.…”

Section: Introductionmentioning

confidence: 99%

“…In the previous research, our group isolated and identified a new indole alkaloid from the aerial parts of Bupleurum chinense DC. [3] In order to continue the further research in this direction, our efforts to conduct a systematic study of the chemical constituents for active natural products resulted in the identifications of one new alkaloid (1) and five known compounds (2 -6) (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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A New Alkaloid from the Aerial Parts of Bupleurum chinense DC.

Xiao

Liu

Sun

et al. 2020

Chemistry & Biodiversity

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A new isoalloxazine alkaloid, named bupleurine A (1), along with five known compounds (2–6), were isolated from the aerial parts of Bupleurum chinense DC. The structure elucidation of the new alkaloid (1) was employed by combining NMR and HR‐MS data with comparison of reference in the literature. Five known compounds (2–6) were isolated from Bupleurum genus for the first time. Additionally, their antiproliferative activities on HeLa cells were evaluated by MTT assay and IC50 of compounds 1 and 4–6 were below 10 μm after treatment for 24 h.

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Cytotoxic triterpenoid glycosides (saikosaponins) from the roots of Bupleurum chinense

Cited by 44 publications

References 46 publications

Overexpression of BcbZIP134 negatively regulates the biosynthesis of saikosaponins

Overexpression of BcbZIP134 negatively regulates the biosynthesis of saikosaponins

Radix Bupleuri: A Review of Traditional Uses, Botany, Phytochemistry, Pharmacology, and Toxicology

A New Alkaloid from the Aerial Parts of Bupleurum chinense DC.

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