Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob.

Reyes, Mariquit M. De Los; Oyong, Glenn G.; Ebajo, Virgilio D.; Ng, Vincent Antonio S.; Shen, Chien‐Chang; Ragasa, Consolacion Y.

doi:10.7324/japs.2015.501104

Cited by 7 publications

(2 citation statements)

References 18 publications

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“…This implies that HCT-116 cells are more responsive to the treatments used. Differences in treatment response between colon cancer cell lines were also observed in previous studies [44][45][46]. It was reported that variations in the expression profiles of genes associated with drug sensitivity in HCT-116 and HT-29 cells could be an important factor dictating how the cells respond to inhibitory compounds [47].…”

Section: Resultsmentioning

confidence: 52%

Cytotoxic Prenylflavanones from Philippine Stingless Bee (Tetragonula biroi Friese) Nests

Reyes¹,

Oyong²,

Ebajo³

et al. 2018

Asian J. Chem.

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INTRODUCTIONPropolis or "bee glue" is a resinous material collected by honeybees from tree buds, sap flows or other botanical exudates and is used for sealing and coating bee hives. For many centuries, aside from its use as food, beverage and food supplement, it has long been used extensively in traditional medicine because of its wide range of therapeutic values for the treatment of common cold, flu, upper respiratory tract infections, as dermatological preparations for wounds, burns, acne, herpes simplex, genitalis and neurodermatitis, as mouthwash and toothpaste to prevent caries and gingivitis and for cosmetic applications [1,2]. Propolis was shown to exhibit a broad spectrum of biological activities which include antimicrobial [3,4], antifungal [5,6], free radical scavenging [7,8], anti-HIV [9,10], anti-inflammatory [11,12] and anti-herpes [13,14]. A recent study reported that propolis, at a concentration of 0.01 % (w/v), exhibited antiproliferative and cytotoxic actions against human fibroblasts proliferation in cell culture, followed by mild cell necrosis [15]. The dichloromethane crude extract and compounds, glyasperin A (1), propolin E (2) and propolin A (3), obtained from Philippine stingless bee (Tetragonula biroi Friese) nests, were evaluated for their cytotoxic potentials. The anti-proliferative activities of crude extract and compounds 1-3 against human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast neonatal (HDFn), were evaluated using the PrestoBlue ® cell viability assay. The crude extract was most anti-proliferative against HCT-116 cells (IC50 = 1.410 µg/mL). Glyasperin A (1) exhibited the strongest effect on MCF-7 cells (2.378 µg/mL). Propolin E (2) was most cytotoxic against HCT-116 cells (2.279 µg/mL), while propolin A (3) was most inhibitory against MCF-7 cells (2.815 µg/mL). Comparing the colorectal cancer cell lines, HCT-116 was generally more susceptible under crude extract, 2, and 3 (IC50 = 1.410, 2.279, 3.013 µg/mL, respectively) than 5.320, 5.359). Comparing the activities of propolin E and propolin A against HCT-116 cells, 2 (IC50 = 2.279 µg/mL) was more cytotoxic than 3 (3.013). The crude extract was more cytotoxic than Zeocin against HCT-116 cells. To our best of knowledge, this is the first report on the anti-proliferative properties of crude extract and compounds from T. biroi.

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Section: Resultsmentioning

confidence: 52%

Cytotoxic Prenylflavanones from Philippine Stingless Bee (Tetragonula biroi Friese) Nests

Reyes¹,

Oyong²,

Ebajo³

et al. 2018

Asian J. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…A difference in treatment responses between two colon cancer cell lines was also seen in previous studies. 16,17 It was reported that changes in the expression profiles of genes associated with drug sensitivity between HCT-116 and HT-29 could influence how the cells react to different inhibitory compounds. 18 A similar study using four human colon cancer cell lines (HCT-116, HT-29, HCT-15, and KM-12) showed that gene expression profiling after inhibition of signal transduction by 17-allylamino-17-demethoxygeldanamycin, a known inhibitor of the hsp90 molecular chaperone, could explain the cells' response under different treatment parameters.…”

Section: Results and Discusionmentioning

confidence: 99%