Cytotoxic Properties of Some Medicinal Plant Extracts from Mazandaran, Iran

Nemati, Farkhondeh; Dehpouri, Abbas Ali; Eslami, B; Mahdavi, V.R.; Mirzanejad, Sepideh

doi:10.5812/ircmj.8871

Cited by 44 publications

(32 citation statements)

References 4 publications

(4 reference statements)

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“…Dilution of stock solutions was made in the culture medium yielding final extract concentrations in the DMSO concentration of 0.1 %. This concentration of DMSO did not affect the cell viability [20]. DMSO was used as the solvent control.…”

Section: Mtt Assay For Assessment Of Cell Viabilitymentioning

confidence: 99%

In Vitro Antibacterial Activity of Selected Underutilized Plants and Cytotoxic Property of Terminalia Catappa

Somarathna

Premakumara

Mohammad

et al. 2017

Int J Pharm Pharm Sci

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Objective: This study aimed at determining the antibacterial activity of the underutilized plants of Sri Lanka, “Kottamba” (Terminalia catappa), “Purpurata” (Alpinia purpurata) and “Harankaha” (Curcuma zedoaria), against food-borne pathogens. Chemical composition and in vitro cytotxicity of the most active antibacterial plant extract(s) were examined.Methods: Crude rhizome extracts were obtained for all plants whereas in respect of T. catappa, the red pericarp of the fruit was used. The antibacterial activity was determined using the agar disc diffusion and broth dilution assays. Total phenol content, Gas Chromatograph-Mass Spectrometry analysis and cytotoxicity assay were conducted only with the plant which showed the most effective antibacterial activity.Results: T. catappa extract showed significantly (p<0.05) high DIZ (19.6±0.47 mm) against S. aureus 113. A. purpurata showed DIZ (16.3±0.94, 15.0±1.00, 14.3±0.57 mm) against L. monocytogenes V7 (1/2a), S. aureus 25925 and S. aureus MSSASS 25D. The MIC of T. catappa ethanol extract was 10 mg/ml, while MBC was 80 mg/ml for S. aureus 113. The phenolic content of T. catappa ethanol extract was 81.54±1.28 mg GAE/g dry sample and the major compound (31.86 %) was 2, 5-Furandione, 3 methyl. The No-Observed Adverse Effect Concentration (NOAEC) of this extract for COS7 cells was 100 µg/ml whereas for 3T3 it was 300 µg/ml. This indicates that the extract is cytotoxic only at a very high concentration, suggesting that at lower concentrations the extract could be used as a food preservative.Conclusion: The results indicate that T. catappa has potential antibacterial activity as a safe bio-preservative.

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Section: Mtt Assay For Assessment Of Cell Viabilitymentioning

confidence: 99%

In Vitro Antibacterial Activity of Selected Underutilized Plants and Cytotoxic Property of Terminalia Catappa

Somarathna

Premakumara

Mohammad

et al. 2017

Int J Pharm Pharm Sci

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“…The different isolated compounds were dissolved in DMSO and then diluted in medium containing DMSO to achieve a range of concentrations of each compound, all with a final treatment concentration of DMSO of 0.01% (including the negative control). These doses of each compound were then used to treat the cells for 24 hr before assessment using the MTT assay (Mosmann, ; Nemati, Dehpouri, Eslami, Mahdavi, & Mirzanejad, ). Briefly, each treatment dose was removed from the 24‐well plates of cells and replaced with MTT solution (0.5 mg/ml MTT in medium 1 ml/well).…”

Section: Methodsmentioning

confidence: 99%

Growth inhibitory activity of biflavonoids and diterpenoids from the leaves of the Libyan Juniperus phoenicea against human cancer cells

Groshi

Jasim

Evans

et al. 2019

Phytotherapy Research

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Three biflavonoids [cupressuflavone (1), amentoflavone (2), and sumaflavone (3)], four diterpenoids [13-epi-cupressic acid (4), imbricatholic acid (5), 3-hydroxysandaracopimaric acid (6), and dehydroabietic acid (7)], and one lignan [β-peltatin methyl ether (8)] were isolated from the cytotoxic fractions of the extracts of the leaves of the Libyan Juniperus phoenicea L. The structures of these compounds were elucidated by spectroscopic means. Cytotoxicity of compounds 1-6 were assessed against the human lung cancer cell line A549 using the MTT assay. Compounds 1 and 3 showed cytotoxicity against the A549 cells (IC 50 = 65 and 77 μM, respectively), whereas compound 2 did not show any activity. Diterpenes 4-6 exhibited weak cytotoxicity against the A549 cells with the IC 50 values of 159, 263, and 223 μM, respectively. The cytotoxicity of each compound was compared with the anticancer drug, etoposide (IC 50 = 61 μM). Cupressuflavone (1) was evaluated also for cytotoxicity against both the human PC3 cancer cell line and the normal prostate cell line (PNT2), and this compound revealed a high degree of cytotoxic selectivity towards the prostate cancer cells (PC3), with IC 50 value of 19.9 μM, without any evidence of cytotoxicity towards the normal prostate cell line (PNT2).

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“…CC50 of all the plant extracts was determined to be [1000 lg/ml. Nemati et al (2013) studied the cytotoxic properties of ethanolic extracts of some medicinal plants (Consolida orientalis, Ferula assafoetida, Coronilla varia and Orobanche orientalis) on HeLa cell line in vitro. The study evaluated that the extracts of C. orientalis, F. assafoetida and C. varia had potential cytotoxic activity on HeLa cell line but O. orientalis showed no significant growth inhibition.…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic potential of Calotropis procera, Azadirachta indica and Punica granatum against Gastrothylax indicus

et al. 2015

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Anthelmintic activity of both ethanolic and aqueous extracts of Calotropis procera flowers, Azadirachta indica leaves and Punica granatum fruit peel in comparison with albendazole was evaluated through in vitro studies by the worm motility inhibition assay. Significant anthelmintic effects (p \ 0.0005) were observed on live Gastrothylax indicus worm as evident from their mortality at 4 h post exposure to both ethanolic and aqueous extracts. Phytochemical analysis of extracts revealed the presence of phenols, alkaloids, saponins, tannins, flavonoids, steroids and triterpenoids. LC-50 values were determined to be 12.05 mg/ml ± 3.24 and 23.52 mg/ ml ± 6.4 for C. procera, 24.37 mg/ml ± 4.11 and 21.02 mg/ml ± 4.6 for A. indica, 18.92 mg/ml ± 4.54 and 24.43 mg/ml ± 6.96 for P. granatum ethanolic and aqueous extracts respectively, whereas it was 29.23 lg/ ml ± 4.51 for albendazole. The mean mortality index (MI) was 1.0 and 0.90 for C. procera, 0.90 for A. indica and 0.73 and 0.80 for P. granatum ethanolic and aqueous extracts respectively whereas for albendazole it was 1.0. Percent mean worm motility inhibition (%WMI) was observed to be between 70 and 100 % for different extracts.Various concentrations (5-5000 lg/ml) of all the plant extracts and albendazole were used to detect their cytotoxic effects against HeLa cell line to determine CC-50 by MTT assay. CC-50 values, of all the plant extracts were determined to be [1000 lg/ml and for albendazole it was found to be [10 lM. All the three plants can be potential sources for novel anthelmintics.

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Cytotoxic Properties of Some Medicinal Plant Extracts from Mazandaran, Iran

Cited by 44 publications

References 4 publications

In Vitro Antibacterial Activity of Selected Underutilized Plants and Cytotoxic Property of Terminalia Catappa

In Vitro Antibacterial Activity of Selected Underutilized Plants and Cytotoxic Property of Terminalia Catappa

Growth inhibitory activity of biflavonoids and diterpenoids from the leaves of the Libyan Juniperus phoenicea against human cancer cells

Anthelmintic potential of Calotropis procera, Azadirachta indica and Punica granatum against Gastrothylax indicus

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