Cytotoxic Lymphoid Cells and Antibodies from Guinea Pigs Immunized with Tubercle Bacilli

Wasserman, Jerzy; Packalén, Thorolf; Perlmann, Peter; Perlmann, Hedvig

doi:10.1159/000230730

Cited by 17 publications

(12 citation statements)

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“…Humoral antibodies have been shown, in several models, to mediate a lymphocyte induced destruction of antigenic cells [5,10,18,27,28]. A sim ilar serum born reactivity was also found in the present study and was well correlated to the occurrence of thyroiditis.…”

Section: Discussionsupporting

confidence: 85%

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Cellular and Humoral Immune Responses in Experimental Autoimmune Thyroiditis

Ringertz¹,

Wasserman²,

Packalén³

et al. 1971

Int Arch Allergy Immunol

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Guinea pigs were immunized once with homologous thyroglobulin in complete Freund’s adjuvant and observed for lesions in the thyroid gland as well as for different parameters of the immune response to thyroglobulin. In individual animals there was a significant correlation between the development of thyroiditis and delayed type skin hypersensitivity to thyroglobulin and the inhibition of migration of peritoneal exudate cells by thyroglobulin. Thyroid lesions and haemagglutinating antibodies to thyroglobulin were less well correlated. Lymphoid spleen cells from immunized animals lysed thyroglobulin coated chicken erythrocytes. Sera from these animals contained antibodies which in the absence of complement rendered lymphocytes from normal guinea pigs cytotoxic for thyroglobulin coated chicken erythrocytes. Presence of thyroid lesions was correlated to both these phenomena. The incidence of thyroiditis and cytotoxicity of immune spleen cells reached their peak 3–4 weeks after immunization and then decreased. The inhibition of macrophage migration reached its peak before the establishment of thyroid lesions and decreased at the same time as the incidence of thyroiditis decreased. Skin reactivity, haemagglutinating antibodies and antibody induced cytotoxicity of normal lymphocytes remained unchanged during the observation period.

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Section: Discussionsupporting

confidence: 85%

“…The cytotoxicity of spleen cells from normal animals induced by antiserum from im munized animals [27,28] was determined as follows. Either crude spleen cells or purified spleen lymphocytes from non-immunized donors were used in these experiments.…”

Section: Cytotoxicity Testsmentioning

confidence: 99%

Cellular and Humoral Immune Responses in Experimental Autoimmune Thyroiditis

Ringertz¹,

Wasserman²,

Packalén³

et al. 1971

Int Arch Allergy Immunol

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“…It has been shown in several model systems that humoral antibodies may either block cell-mediated cytotoxicity in vitro of lymphocytes from sensitized donors, or may induce lymphocytes from normal donors to become cytotoxic to anti body carrying target cells [11], Evidence for the occurrence of the latter phenomenon in PPD reactive guinea pigs will be presented in this paper. Preliminary reports of some of these data have been presented [10,18].…”

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confidence: 99%

Antibody Induced Lysis of PPD-coated Chicken Erythrocytes by Spleen Cells of Non-Immunized Guinea Pigs

Wasserman¹,

Packalén²,

Perlmann³

et al. 1971

Int Arch Allergy Immunol

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Chicken erythrocytes coated with PPD were labelled with ⁵¹Cr. When incubated with spleen cells from non-immunized animals in the presence of heat inactivated serum from immunized guinea pigs, the red cells were lysed as assessed by isotope release. The reaction was highly specific and required direct contact between target cells and spleen cells. Cytotoxicity of normal spleen cells was observed after preincubation of the PPD-coated erythrocytes, or when immune serum was present in the incubation mixture. Spleen cells pretreated with immune serum exhibited an activity of low degree. Sera from animals injected with tubercle bacilli or with PPD in incomplete Freund’s adjuvant were active, whereas sera from animals immunized with alum precipitated PPD were of weak or doubtful activity. The stimulating activity of sera increased with the intensity of immunization. Cytotoxicity inducing activity and haemagglutinating antibody titers were roughly correlated in animals injected with PPD but not in animals immunized with tubercle bacilli.

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“…MBP (75 pg/ml), or histone (75 pg/ml) by the method of Wasserman et al [1969]. In brief, a 1% stock solution of tannic acid in PBS (pH 7.2) was prepared weekly, sterilized by filtration and stored at 4 °C.…”

Section: Target Cellsmentioning

confidence: 99%

Optic Neuritis and Multiple Sclerosis

Frick¹

1988

Eur Neurol

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On examination in the first weeks after onset of the disease, cell-mediated cytotoxicity against encephalitogenic peptide, myelin basic protein (MBP), cerebrosides and gangliosides was demonstrable in 32 (76%) of 42 patients with optic neuritis (ON). 14 patients with ON and concomitant neurological symptoms (ON/MS) had positive findings with all antigens, especially with the encephalitogenic peptide. The cytotoxic reactions against all antigens showed a close correlation with the course of ON: when the disease improved, the cytotoxicity decreased and became negative on average 4 months after ON onset. As previously reported, cell-mediated cytotoxicity against the above-mentioned antigens, especially the encephalitogenic peptide, may be considered as a pathogenetic factor in multiple sclerosis (MS). A positive reaction with this antigen in patients with ON only probably indicates the first manifestation of MS.

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Cytotoxic Lymphoid Cells and Antibodies from Guinea Pigs Immunized with Tubercle Bacilli

Cited by 17 publications

References 0 publications

Cellular and Humoral Immune Responses in Experimental Autoimmune Thyroiditis

Cellular and Humoral Immune Responses in Experimental Autoimmune Thyroiditis

Antibody Induced Lysis of PPD-coated Chicken Erythrocytes by Spleen Cells of Non-Immunized Guinea Pigs

Optic Neuritis and Multiple Sclerosis

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