Cytotoxic Interactions of Virus Specific Effector Cells With Virus Infected Targets of Different Cell Type*

Koszinowski, Ulrich H.; Ertl, Hildegund C. J.

doi:10.1111/j.1744-313x.1977.tb00620.x

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Virussensitized mouse T lymphocytes will only lyse target cells infected with related or homologous virus preparations if both effector and target cells share the K or D region of the H-2 gene complex, or both, and express the respective gene products. This observation has been described by many authors (10, 12,13,15,16,22,23,30,34,43,44). The assay systems usually involve a short-term incubation period (around 4 h) for the effector and target cell mixture and absence of active complement.…”

Section: Resultsmentioning

confidence: 66%

“…The aim of this study was to provide the means for measuring T-and B-cell responses in parallel in an easy way using the same assay system. It has been amply demonstrated that in mice antiviral activity of cytotoxic T cells is restricted by products of the major histocompatibility gene complex, H-2 (10,12,13,15,16,22,23,30,34,43,44), whereas cytolytic activity of B-cell products is not. Use of H-2-identical or -different effector-target combinations in the assay should allow discrimination between both types of responses.…”

mentioning

confidence: 99%

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Induction of cytolytic T- and B-cell responses against influenza virus infections

Armerding¹,

Rossiter²

1980

Infect Immun

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Inoculation of mice with live influenza virus results in the induction of cytotoxic thymus-derived (T) lymphocytes and of bone marrow-derived (B) cells producing antiviral antibodies. An assay system was developed to evaluate both types of immune responses on a cellular basis within the same lymphocyte pool with no need to separate out the different effector cell classes. The test system represented a modification of the 51Cr-release assay. T-cell activity was measured exclusively in the absence of active complement using targets that were compatible for determinants encoded by the mouse major histocompatibility gene complex, H-2. H-2-different and even xenogeneic target cells were lysed in the presence of either non-inactivated fetal calf serum or normal rabbit serum as a complement source. Cytotoxicity was mediated in the latter case by direct interaction of B-cell-produced immunoglobulin directed to viral antigens expressed by the target cell and complement. Antibody-dependent cell-mediated cytotoxicity mechanisms did not contribute to cytotoxicity in the test system described. It was demontrated that the cytolytic B-cell responses of one particular strain of mice (BALB/c) against different influenza A viruses were restricted to the immunizing virus on the effector cell level. In another strain of mice (C3H), B cells revealed a broad cross-reactive response resembling that of killer T cells.

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Section: Resultsmentioning

confidence: 66%

mentioning

confidence: 99%

Induction of cytolytic T- and B-cell responses against influenza virus infections

Armerding¹,

Rossiter²

1980

Infect Immun

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MHC-Restricted Cytotoxic T Cells: Studies on the Biological Role of Polymorphic Major Transplantation Antigens Determining T-Cell Restriction-Specificity, Function, and Responsiveness

Zinkernagel

Doherty

1979

Advances in Immunology

1,759

615

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IMMUNOGENETIC STUDIES ON METH‐A‐VACCINIA TUMOUR CELLS IN VIVO AND *IN VITRO**

Garrido

Schmidt

Festenstein

1977

Int J Immunogenetics

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SUMMARY The expression of H‐2 antigenic specificities on Meth‐A and meth‐A‐vaccinia were compared. Their ability to inhibit complement dependent cytotoxicity of 51Cr‐labelled normal lymphoid target cells was tested by anti H‐2 sera of restricted specificity. Normal lymphocytes positive or negative for a particular specificity, were used as controls. With this assay the expression of H‐2‐like specificities of foreign haplotypes on Meth‐A cells passaged together with vaccinia virus was confirmed. In addition, using an immunofluorescence technique the expression of some foreign H‐2 antigens on non‐infected Meth‐A tumour cells was shown. Anti‐vaccinia virus sera were used to compare the reactivity of Meth‐A and Meth‐A‐vaccinia. Unexpectedly, anti‐vaccinia sera stained non‐infected Meth‐A and other tumour cells of different H‐2 origin and absorption of these sera with normal Meth‐A left little activity behind when testing against Meth‐A‐vaccinia. Finally, the rǒle that the foreign H‐2 antigenic specificities present in Meth‐A after vaccinia virus infection may have in the growth of these tumour cells in syngeneic recipients was studied. A powerful inhibitory effect was observed and regressor mice were found to be resistant to a challenge of non‐infected meth‐A. In contrast, vaccinia virus was irrelevant to the rate of growth of P815 Y in DBA/2 mice. These results are discussed in relation to the origin of the foreign H‐2 antigens on tumour cells after virus infection (derepression) and the role that this new antigenicity could have in immunopotentiation.

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Cytotoxic Interactions of Virus Specific Effector Cells With Virus Infected Targets of Different Cell Type*

Cited by 3 publications

References 24 publications

Induction of cytolytic T- and B-cell responses against influenza virus infections

Induction of cytolytic T- and B-cell responses against influenza virus infections

MHC-Restricted Cytotoxic T Cells: Studies on the Biological Role of Polymorphic Major Transplantation Antigens Determining T-Cell Restriction-Specificity, Function, and Responsiveness

IMMUNOGENETIC STUDIES ON METH‐A‐VACCINIA TUMOUR CELLS IN VIVO AND *IN VITRO**

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Cytotoxic Interactions of Virus Specific Effector Cells With Virus Infected Targets of Different Cell Type*

Cited by 3 publications

References 24 publications

Induction of cytolytic T- and B-cell responses against influenza virus infections

Induction of cytolytic T- and B-cell responses against influenza virus infections

MHC-Restricted Cytotoxic T Cells: Studies on the Biological Role of Polymorphic Major Transplantation Antigens Determining T-Cell Restriction-Specificity, Function, and Responsiveness

IMMUNOGENETIC STUDIES ON METH‐A‐VACCINIA TUMOUR CELLS IN VIVO AND IN VITRO*

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Product

Resources

About

IMMUNOGENETIC STUDIES ON METH‐A‐VACCINIA TUMOUR CELLS IN VIVO AND *IN VITRO**