Cytotoxic, Genotoxic and pro-apoptotic effect of some medicinal plants on the expression of colon cancer-related genes (P53 &amp; Bcl2) in the colorectal cell line and syngenic animal cancer model

Mahmoud, Khaled; Mohammed, Aya; Salem, D; Ibrahim, Karim Yaqub; Shawky, Mario; Osama, Mohamed; Rageh, Mostafa; Tarek, Rana; Shahed, Zeinab El

doi:10.21608/ejchem.2022.138368.6087

Cited by 4 publications

(1 citation statement)

References 37 publications

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“…Doxorubicin served as positive control and DMSO (0.5 %) was served as negative control. The cytotoxicity was evaluated using the MTT assay and according to the following equation: % cytotoxicity = [1-(AVx / AVNC)] x 100, Where AVx: average absorbance of sample wells and AVNC: average absorbance of negative control wells measured at 595 nm with reference at 690 nm [29].…”

Section: Cytotoxicity Bioassay On Bj1 Cell Linementioning

confidence: 99%

Chemical Composition and Bioactivities of Egyptian Opuntia ficus–indica Seeds Oils Obtained by Conventional and Ultrasound-assisted Extraction Techniques

et al. 2023

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The main objective of the current work was to assess the suitability of ultrasound-assisted extraction (UAE) for the oil recovery from Opuntia ficus-indica (L.) Mill. (O. ficus indica) seeds as compared to conventional extraction technique; Soxhlet extraction (SE). In the present study, the recovery oil yield, the saponifiable and unsaponifiable lipids of seeds oils obtained by different extraction methods, were compared. In addition, determination of the wound healing and analgesic activities of the plant seed oil, in vitro and in vivo, were evaluated. UAE and SE methods recovered total oil yields of 2.15% and 2.01%; respectively. Study of fatty acids composition exposed that the predominant fatty acids were linoleic acid (75.2% and 84.9%), oleic acid (14.01% and 8.18%) and palmitic acid (8.91% and 5.71%) for UAE and SE, respectively. Moreover, the most abundant sterol was β-Sitosterol (73.41% and 72.12%) followed by campesterol (8.84% and 8.57%) for UAE and SE, respectively. UAE significantly improved bioactivities of the oil with a significant increase in wound healing rate (85.7%) and inhibition of abdominal writhing (71.0%) compared to those obtained for oil prepared by SE (73.3% and 28.1%, respectively). Conclusion: Compared to conventional extraction method SE, the application of the innovative technique UAE for oil extraction increased the obtained oil yield of O. ficus indica and enhanced the biological activities in-vitro as well as in-vivo.

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Section: Cytotoxicity Bioassay On Bj1 Cell Linementioning

confidence: 99%

Chemical Composition and Bioactivities of Egyptian Opuntia ficus–indica Seeds Oils Obtained by Conventional and Ultrasound-assisted Extraction Techniques

et al. 2023

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Management of Rumex dentatus L. in Vicia faba L. cultivations via Ononis vaginalis Vahl. As a potential bioherbicide

Elghobashy,

Rashed,

Fakhry

et al. 2024

Heliyon

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Synthesis, molecular docking, and anticancer effect of newly synthesized 2- (thiophen-2-yl)-1H-indole derivatives: suppression of HCT-116 Cell proliferation, cell cycle arrest and downregulation of miR-25, C-Myc and IL-6

Abdelazeem,

Gouhar,

Fahmy

et al. 2023

Preprint

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In the present study, we prepared new sixteen different derivatives. The first series were prepared (methylene)bis(2-(thiophen-2-yl)-1H-indole) derivatives which have (indole and thiophene rings) by excellent yield from the reaction (2 mmol) 2-(thiophen-2-yl)-1H-indole and (1 mmol) from aldehyde. The second series were synthesized (2-(thiophen-2-yl)-1H-indol-3-yl) methyl) aniline derivatives by at a relatively low yield from multicomponent reaction of three components 2-(thiophen-2-yl)-1H-indole, N-methylaniline and desired aldehydes. The anticancer effect of the newly synthesized derivatives was determined against different cancers, colon, lung, breast and skin. The counter screening was done against normal Epithelial cells (RPE-1). The effect on cell cycle and mechanisms underlying the antitumor effect were also studied. The initial single dose (100µg/ml) testing for all new compounds against this panel of 5 human tumor cell lines indicated that the compounds under investigation exhibited selective cytotoxicity against HCT-116 cell line and compounds (4g, 4a, 4c) showed potent anticancer activity against HCT-116 cell line with an IC50 values of 3.566, 5.087, 6.183 µg/ml respectively. Also, the active derivatives caused cell cycle arrest at the S and G2/M phase with significant(p < 0.0001) increase in the expression levels of tumor suppressors miR-30C, and miR-107 and a tremendous decrease in oncogenic miR-25, IL-6 and C-Myc levels. It is to conclude that the anticancer activity could be through direct interaction with tumor cell DNA like S-phase-dependent chemotherapy drugs. Which can interact with DNA or block DNA synthesis such as doxorubicin, cisplatin, or 5-fluorouracil and which were highly effective in killing the cancer cells. This data ensures the efficiency of the 3 analogues on inducing cell cycle arrest and preventing cancer cell growth. The altered expressions explained the molecular mechanisms through which the newly synthesized analogues exert their anticancer action.

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Cytotoxic, Genotoxic and pro-apoptotic effect of some medicinal plants on the expression of colon cancer-related genes (P53 & Bcl2) in the colorectal cell line and syngenic animal cancer model

Cited by 4 publications

References 37 publications

Chemical Composition and Bioactivities of Egyptian Opuntia ficus–indica Seeds Oils Obtained by Conventional and Ultrasound-assisted Extraction Techniques

Chemical Composition and Bioactivities of Egyptian Opuntia ficus–indica Seeds Oils Obtained by Conventional and Ultrasound-assisted Extraction Techniques

Management of Rumex dentatus L. in Vicia faba L. cultivations via Ononis vaginalis Vahl. As a potential bioherbicide

Synthesis, molecular docking, and anticancer effect of newly synthesized 2- (thiophen-2-yl)-1H-indole derivatives: suppression of HCT-116 Cell proliferation, cell cycle arrest and downregulation of miR-25, C-Myc and IL-6

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