Cytotoxic effects on tumour cell lines of fatty acids from the marine sponge <i>S</i>
                  <i>copalina ruetzleri</i>

Biegelmeyer, Renata; Schröder, Rafael; Rambo, Douglas Fernando; Dresch, Roger Remy; Stout, E. Paige; Carraro, João Luís; Mothes, Beatriz; Moreira, José Cláudio Fonseca; Molinski, Tadeusz F.; Frota, Mario Luiz Conte da; Henriques, Amélia Teresinha

doi:10.1111/jphp.12366

Cited by 6 publications

(15 citation statements)

References 22 publications

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Section: Spectroscopic Data Of Fasunclassified

“…Tetradecanoic acid (myristic acid) ( (14), 147 (9), 133 (15), 119 (20), 106 (28), 105 (35), 93 (47), 91 (74), 79 (100), 67 (69), 55 (39). (9), 145 (12), 135 (7), 131 (22), 128 (10), 119 (39), 117 (27), 105 (50), 95 (17), 93 (45), 91 (87), 80 (36), 79 (100), 77 (42), 67 (53), 55 (31). (7), 166 (5), 152 (4.5), 139 (9), 124 (6.7), 111 (13.4), 97 (31.9), 87 (36), 83 (37.5), 74 (49.3), 69 (59.8), 67 (30.5) (5), 180 (4), 175 (5), 150 (14), 147 (9), 133 (15), 119 (20), 106 (28), 105 (35), 93 (47), 91 (74), 79 (100), 67 (69), 55 (39).…”

Section: Spectroscopic Data Of Fasunclassified

“…The lipid compositions of twenty genera of marine sponges from the Demospongiae class were analyzed and shown to contain unusually high levels of C 24 -C 30 (34-79%), and the sponges specific FAs [8]. Some sponges' lipids are known as biologically active constituents [3,4,7,9]. For instance, mixtures of Δ 5,9 -unsaturated fatty acid (UFAs) have cytotoxic activity against mouse Ehrlich carcinoma cells and a hemolytic effect on mouse erythrocytes [10].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, mixtures of Δ 5,9 -unsaturated fatty acid (UFAs) have cytotoxic activity against mouse Ehrlich carcinoma cells and a hemolytic effect on mouse erythrocytes [10]. Additionally, the cytotoxicity of FAs isolated from marine sponges were evaluated on human glioma (U87) and neuroblastoma (SH-SY5Y) cell lines, and showed that FAs are potential cytotoxic metabolites [9].…”

Section: Introductionmentioning

confidence: 99%

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In vitro anti-proliferative activities of the sterols and fatty acids isolated from the Persian Gulf sponge; Axinella sinoxea

et al. 2019

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Purpose Marine sponges are rich sources of anticancer metabolites. Axinella sinoxea is a less studied sponge, found in the Larak Island's waters, of the Persian Gulf. In the present study, we have explored the cytotoxic properties and chemical constituents of A. sinoxea. Methods Repeated silica gel flash column chromatography of methanol extract of the Axinella sinoxea sponge, yielded fatty acid and sterol fractions. These fractions were analyzed by GC-MS and their anti-proliferative activities were evaluated by MTT assay against three human cancer cell lines including MOLT-4, MCF-7 and HT-29 as well as NIH/3 T3 fibroblast cells. The sterol-rich fractions were pooled and purified by HPLC and its sub fractions' cytotoxic activities were evaluated by MTT assay against MOLT-4 and NIH/3 T3 cells. ResultsThe GC-MS spectral analysis of a fraction eluted with hexane: diethyl ether (90: 10), resulted in the identification of twelve fatty acids, including five linear chain saturated fatty acids; tetrdecanoic acid (1), pentadecanoic acid (3), hexadecanoic acid (5), heptadecanoic acid (7), and octadecanoic acid (10); one branched chain isoprenoid fatty acid, 4,8,12trimethyltridecanoic acid (2); four monoenoic fatty acids; 9-hexadecenoic acid (4), 7-methyl-6-hexadecanoic acid (6), 9octadecenoic acid (8) and 11-octadecenoic acid (9) and two polyunsaturated fatty acids; 5,8,11,14-eicosatetraenoic acid (11) and 4,7,10,13,16,19-docosahexaenoic acid (12). Spectral analysis of a non-polar fraction eluted with hexane: diethyl ether (85: 15), resulted in the identification of eight steroids including: cholesta-5,22-dien-3β-ol (13), cholest-5-en-3β-ol (14), ergosta-5,22-dien-3β-ol (15), ergost-5-en-3β-ol (16), stigmasta-5,22-dien-3β-ol (17), γ-sitosterol (18), 33-norgorgosta-5,24(28)-dien-3βol (19) and stigmasta-5,24(28)-dien-3β-ol (20). Fatty acids-containing fraction was active against HT-29 cell line with IC 50 26.52 ± 8.19 μg/mL, while the steroids-rich fraction was active against the three above mentioned cell lines with IC 50 values of 1.20 ± 0.24, 4.12 ± 0.40 and 2.47 ± 0.31 μg/mL, respectively. All of the above-mentioned fractions and sub-fractions were inactive (IC 50 s > 50 μg/mL) when assayed against normal fibroblast cells. Conclusion The present study suggests A. sinoxea as a potential natural source of cancer chemotherapeutics.

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Section: Spectroscopic Data Of Fasunclassified

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro anti-proliferative activities of the sterols and fatty acids isolated from the Persian Gulf sponge; Axinella sinoxea

et al. 2019

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“…Moreover, 9,12-Octadecadienoic acid, fatty acid, was also identified from other marine sponge, Scopalina ruetzleri, which collected from South Brazilian coastline. Ethyl acetate fraction of S. ruetzleri induces cell death of human glioma cells and neuroblastoma cells (Biegelmeyer et al, 2015).…”

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confidence: 99%

Bioactive Compounds in the Ethanol Extract of Marine Sponge Stylissa carteri Demonstrates Potential Anti-Cancer Activity in Breast Cancer Cells

Bashari

Huda

Tartila

et al. 2019

Asian Pac J Cancer Prev

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Objective: Despite advanced treatment options available, drug resistance develops in breast cancer (BC) patients requiring novel effective drugs. Stylissa carteri, a marine sponge predominantly living in Indonesia territories, has not been extensively studied as anti-cancer. Therefore, this study targeted to assess the anti-tumor activity of the ethanol extract of S. carteri in BC cells. Methods: S. carteri was collected from Pramuka Island, at Kepulauan Seribu National Park, Jakarta, Indonesia and extracted using ethanol. Different BC cells including MDA MB 231, MDA MB 468, SKBR3, HCC-1954 and MCF-7 cells were treated with this extract for cytotoxic analysis using MTT assay. Spheroid growth assay and apoptosis assay were conducted in HCC-1954 cells. In addition, cell migration analysis and synergistic activity with doxorubicin or paclitaxel were conducted in MDA MB 231 cells. This extract was subjected also for GC-MS analysis. Results: The results show that ethanol extract of S. carteri demonstrated a cytotoxic activity in BC cells. The IC 50 of this extract was lower 15 μg/ml in MDA MB 231, MDA MB 468, SKBR3, and HCC-1954 cells. Moreover, this extract inhibited spheroids growth and induced apoptosis in HCC-1954 cells. It inhibited cell migration and demonstrated a synergistic activity with doxorubicin or paclitaxel on triggering cell death in MDA MB 231 cells. Furthermore, GC-MS analysis indicated that this extract contained 1,2-Benzenediol, Dibutyl phthalate and 9,12-Octadecadienoic acid, ethyl ester. Conclusion: Our preliminary data indicate a potential anti-tumor activity of ethanol extract of S. carteri in breast cancer cells.

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Sponges from Zanzibar host diverse prokaryotic communities with potential for natural product synthesis

Helber

Steinert

et al. 2019

FEMS Microbiology Ecology

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Sponges are one of the most dominant organisms in marine ecosystems. One reason for their success is their association with microorganisms that are besides the host itself responsible for the chemical defence. Sponge abundances have been increasing on coral reefs in the Western Indian Ocean (WIO) and are predicted to increase further with rising anthropogenic impacts on coral reefs. However, there is a paucity of information on chemical ecology of sponges from the WIO and their prokaryotic community composition. We used a combination of Illumina sequencing and a predictive metagenomic analysis to (1) assess the prokaryotic community composition of sponges from Zanzibar, (2) predict the presence of KEGG metabolic pathways responsible for bioactive compound production and (3) relate their presence to the degree of observed chemical defence in their respective sponge host. We found that sponges from Zanzibar host diverse prokaryotic communities that are host speciesspecific. Sponge-species and respective specimens that showed strong chemical defences in previous studies were also predicted to be highly enriched in various pathways responsible for secondary metabolite production. Hence, the combined sequencing and predictive metagenomic approach proved to be a useful indicator for the metabolic potential of sponge holobionts.

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Cytotoxic effects on tumour cell lines of fatty acids from the marine sponge S copalina ruetzleri

Abstract: This work also contributes to studies that focus on the application of FAs on cancer therapy as a new adjuvant to radio or chemotherapy, or as a chemotherapeutic agent.

Cited by 6 publications

References 22 publications

In vitro anti-proliferative activities of the sterols and fatty acids isolated from the Persian Gulf sponge; Axinella sinoxea

In vitro anti-proliferative activities of the sterols and fatty acids isolated from the Persian Gulf sponge; Axinella sinoxea

Bioactive Compounds in the Ethanol Extract of Marine Sponge Stylissa carteri Demonstrates Potential Anti-Cancer Activity in Breast Cancer Cells

Sponges from Zanzibar host diverse prokaryotic communities with potential for natural product synthesis

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