Cytotoxic effects of stem bark extracts and pure compounds from Margaritaria discoidea on human ovarian cancer cell lines

Johnson-Ajinwo, Okiemute Rosa; Richardson, Alan; Li, Wen‐Wu

doi:10.1016/j.phymed.2014.09.008

Cited by 30 publications

(22 citation statements)

References 15 publications

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“…The Sulforhodamine B (SRB) assay (Johnson‐Ajinwo et al ., ) was used to evaluate the effects of the plant extracts and pure compounds on the growth of ovarian cancer cell lines (Ovcar‐8, A2780, Ovcar‐4, Igrov‐1) and human normal OSE cells. 2000 cells (A2780, Ovcar‐8) and 5000 (Ovcar‐4, Igrov‐1, or OSE) cells were seeded in 80 μL of growth medium in each well of a 96‐well plate.…”

Section: Methodsmentioning

confidence: 99%

“…However, no anticancer activity of extracts of T. subcordata constituent alkaloids or further phytochemical study has been reported. Therefore, in continuation of our search for anticancer compounds from medicinal plants (Johnson‐Ajinwo et al ., ; Li et al , ; Li et al ., ), this study aimed to evaluate the in vitro anti‐ovarian cancer activities of the crude extracts and pure compounds of T. subcordata , and bioassay‐guided fractionation was used to isolate the bioactive compound. Furthermore, the cytotoxic effects and possible signalling pathways of cycleanine and tetrandrine involved in their cytotoxity on Ovcar‐8, A2780, Ovcar‐4, and Igrov‐1 ovarian cancer cell lines were investigated.…”

Section: Introductionmentioning

confidence: 98%

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Cytotoxicity Effects and Apoptosis Induction by Bisbenzylisoquinoline Alkaloids fromTriclisia subcordata

et al. 2016

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Triclisia subcordata Oliv (Menispermeaceae) is a medicinal plant traditionally used for the treatment of various diseases in West Africa. The ethanol extract of T. subcordata and its fractions were screened for in vitro anti-ovarian cancer activities using the Sulforhodamine B assay. The crude alkaloids showed the strongest activity in cell growth assays on Ovcar-8 and A2780 cell lines (IC50 < 2.4 µg/mL). A bisbenzylisoquinoline alkaloid-cycleanine was isolated using HPLC and identified by mass spectrometry and nuclear magnetic resonance analyses. The IC50 values of cycleanine and tetrandrine (an alkaloid previously reported from this plant) ranged from 7 to 14 μM on Ovcar-8, A2780, Ovcar-4, and Igrov-1 ovarian cancer cell lines. The IC50 of cycleanine on human normal ovarian surface epithelial cells was 35 ± 1 μM, hinting at modest selectivity toward cancer cells. Both cycleanine and tetrandrine caused apoptosis as shown by activation of caspases 3/7 and cleavage of poly(ADP-ribose) polymerase to form poly(ADP-ribose) polymerase-1 by using western blot analysis. Flow cytometry analyses showed that the percentages of apoptotic cells and cells in subG1 phase increased after exposure of cycleanine and tetrandrine to Ovcar-8 cells for 48 h compared with control. Cycleanine, like its isomer tetrandrine isolated from T. subcordata, could be a potential new anti-ovarian cancer agent acting through the apoptosis pathway. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Cytotoxicity Effects and Apoptosis Induction by Bisbenzylisoquinoline Alkaloids fromTriclisia subcordata

et al. 2016

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“…In order to identify potentially bioactive compounds, GC-MS was initially applied to both CGEEA and CGEBU fractions after trimethylsilylation (Johnson-Ajinwo et al, 2015). These analyses (Figures S2 and S3; Table S1 and S2), indicate that 3,4,5-trihydroxybenzoic acid (gallic acid) as well as several fatty acids were present in both the CGEEA and CGEBU extracts, but also that additional benzoic acid derivatives, including 3-methoxy-4-hydroxybenzoic acid (vanillic acid, 0.4%), 3,4-dihydroxybenzoic acid (protocatechuic acid, 2%), and 3,4,5-trihydroxybenzoic ethyl ester (ethyl gallate, 2%), were also present in CGEEA.…”

Section: Bioassay Guided Separations Of Extract Of Cylicodiscus Gabunmentioning

confidence: 99%

A characterization of the antimalarial activity of the bark of Cylicodiscus gabunensis Harms

Aldulaimi

Uche

Hameed

et al. 2017

Journal of Ethnopharmacology

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Ethnopharmacological relevance and aim: A decoction of the bark of Cylicodiscus gabunensis Harms is used as a traditional medicine in the treatment of malaria in Nigeria. This study aims to validate the antimalarial potency of this decoction in vitro against Plasmodium falciparum and define potential bioactive constituents within the C. gabunensis bark. Materials and methods:A bioassay-guided separation and fractionation protocol was applied to C. 4

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“…Natural products and traditional medicines have been recognized for their major applications in the identication of drug leads and drugs for the treatment of many diseases including ovarian cancer. [1][2][3][4] Numerous phytochemicals and their semi-synthetic derivatives have been approved as anticancer drugs including the well-known paclitaxel, irinotecan, and toptecan. 1 Many others are currently being evaluated in clinical and preclinical studies.…”

Section: Introductionmentioning

confidence: 99%

Isochondodendrine and 2′-norcocsuline: additional alkaloids from Triclisia subcordata induce cytotoxicity and apoptosis in ovarian cancer cell lines

et al. 2017

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Triclisia subcordata Oliv (Menispermeaceae) is used in herbal medicine for the treatment of cancer and other diseases in Africa. This study aims to isolate minor alkaloids present in this plant and assay their cytotoxic activities. Isochondodendrine and 2 0 -norcocsuline as two minor alkaloids together with the abundant cycleanine were isolated and identified by mass spectrometry and NMR spectroscopy. Both isochondodendrine and 2 0 -norcocsuline exhibited in vitro cytotoxicity in four ovarian cancer cell lines (A2780, IGROV-1, OVCAR-8, and OVCAR-4) with IC 50 ranges of 3.5-17 mM and 0.8-6.2 mM respectively.These alkaloids showed mostly slightly weaker potencies when tested using normal human ovarian epithelial cells, IC 50 ¼ 10.5 AE 1.2 mM and 8.0 AE 0.2 mM for isochondodendrine and 2 0 -norcocsuline, respectively. The alkaloids induced apoptosis in ovarian cancer cells because they activated caspases 3/7, induced cleavage of PARP, increased the subG 1 population in cell cycle analysis and increased Annexin V/propidium iodide staining. These observations suggest that isochondodendrine and 2 0 -norcocsuline contributing to the cytotoxic activity of T. subcordata may be suitable starting points for the future development of novel therapeutics to treat ovarian cancer.

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Cytotoxic effects of stem bark extracts and pure compounds from Margaritaria discoidea on human ovarian cancer cell lines

Cited by 30 publications

References 15 publications

Cytotoxicity Effects and Apoptosis Induction by Bisbenzylisoquinoline Alkaloids fromTriclisia subcordata

Cytotoxicity Effects and Apoptosis Induction by Bisbenzylisoquinoline Alkaloids fromTriclisia subcordata

A characterization of the antimalarial activity of the bark of Cylicodiscus gabunensis Harms

Isochondodendrine and 2′-norcocsuline: additional alkaloids from Triclisia subcordata induce cytotoxicity and apoptosis in ovarian cancer cell lines

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