Cytotoxic Effects of Resveratrol, Rutin and Rosmarinic Acid on ARH–77 Human (Multiple Myeloma) Cell Line

Abstract

Multiple myeloma (MM) cancers are 10% of hematological cancers. Leukemia ARH-77 is a malignancy like MM with a worse course of disease and the survival rate from it is very low. Therefore, ARH-77 is a commonly used model for antitumor agent studies. Polyphenolic compounds, such as resveratrol, rutin and rosmarinic acid, have many protective roles, but there is no comparative study about these three polyphenolic compounds on ARH-77. In the present study, we investigated the cytotoxic effects of resveratrol, rutin and rosmarinic acid on ARH-77. Toxic concentration ranges were determined by the brine shrimp lethality test on Artemia salina. In addition, for determination of their cytotoxic effects, MTT and NR methods were used for ARH-77. Resveratrol caused significant reduction in both mitochondrial and lysosomal activities compared with the control group. Maximum inhibition values were detected on mitochondrial and lysosomal activity with 200 µM concentrations after 48 hours. After a 24 hours incubation period, rutin showed cytotoxic effects, particularly with 50, 100 and 200 µM concentrations. Rosmarinic acid also decreased the mitochondrial activity with the same concentrations. Resveratrol showed higher cytotoxic effects than rutin and rosmarinic acid. According to our study, polyphenolic compounds such as rutin, resveratrol and rosmarinic acid may hold promise in multiple myeloma treatment with further investigations.

A Comprehensive Review of Rosmarinic Acid: From Phytochemistry to Pharmacology and Its New Insight

“…In addition, RA also showed an anti-tumor effect on 7,12-dimethylbenz(a)anthracene-induced skin carcinogenesis [ 252 ], a cytotoxic effect on ARH-77 human (multiple myeloma) cells [ 253 ], prostate cancer cells [ 254 ], and human Hep-G2 liver carcinoma cells [ 255 ], and an inhibitory effect on the metastatic properties of colorectal cancer cells [ 256 ].…”

Recent Advances in the Analysis of Rosmarinic Acid From Herbs in the Lamiaceae Family

“…From a chemical point of view, RA (molecular formula: C 18 H 16 O 8 , molecular mass: 360.3 g/mol), a phenylpropanoid, is the caffeic acid ester of 3,4-dihydroxyphenyllactic acid. [23][24][25] Rosmarinic acid contains 2 aromatic amino acids, phenylalanine and tyrosine, which are joined through an ester linkage. The caffeic acid and 3,4-dihydroxyphenyllactic acid are synthesized through 2 distinct routes of the shikimic acid biosynthesis pathway.…”

Prunella vulgaris L. VE Prunella grandiflora L.’DEN SAFLAŞTIRILAN ROSMARİNİK ASİTİN FARKLI TÜMÖR HÜCRELERİ ÜZERİNDEKİ SİTOTOKSİK AKTİVİTESİ

“…The antiproliferative effect of rosmarinic acid on cancer cells might vary with the variation of plant origin. The cytotoxic effect of rosmarinic acid was determined in Leukemia ARH-77 cell line by MTT assay (Canturk et al 2016). Rosmarinic acid showed cytotoxic effects at 50mM concentration.…”