Cytotoxic Effects of Rapana venosa Hemocyanin on Bladder Cancer Permanent Cell Lines

Stenzl, Arnulf; Dolashki, Aleksandar; Stevanović, Stefan; Voelter, Wolfgang; Aicher, Wilhelm K.; Dolashka, Pavlina

doi:10.17265/1548-6648/2016.04.001

Cited by 4 publications

(3 citation statements)

References 19 publications

(35 reference statements)

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“…There is no prior information for FLH or CCH or for these two hemocyanins in combination with QS-21. However, there is evidence that other hemocyanins obtained from Helix aspersa [80] and Rapana venosa [81] have cytotoxic and antiproliferative effects on tumor cell lines such as bladder cancer, ovarian cancer, prostate cancer, and glioma cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

Román

Campo

Villar

et al. 2019

Journal of Immunology Research

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Mollusk hemocyanins have been used for decades in immunological and clinical applications as natural, nontoxic, nonpathogenic, and nonspecific immunostimulants for the treatment of superficial bladder cancer, as carriers/adjuvants of tumor-associated antigens in cancer vaccine development and as adjuvants to dendritic cell-based immunotherapy, because these glycoproteins induce a bias towards Th1 immunity. Here, we analyzed the preclinical therapeutic potential of the traditional keyhole limpet hemocyanin (KLH) and two new hemocyanins from Concholepas concholepas (CCH) and Fissurella latimarginata (FLH) in mouse models of oral squamous cell carcinoma. Due to the aggressiveness and deadly malignant potential of this cancer, the hemocyanins were applied in combination with adjuvants, such as alum, AddaVax, and QS-21, which have been shown to be safe and effective in human vaccines, to potentiate their antitumor activity. The immunogenic performance of the hemocyanins in combination with the adjuvants was compared, and the best formulation was evaluated for its antitumor effects in two murine models of oral cancer: MOC7 cells implanted in the flank (heterotopic) and bioluminescent AT-84 E7 Luc cells implanted in the floor of the mouth (orthotopic). The results demonstrated that the hemocyanins in combination with QS-21 showed the greatest immunogenicity, as reflected by a robust, specific humoral response predominantly characterized by IgG2a antibodies and a sustained cellular response manifesting as a delayed hypersensitivity reaction. The KLH- and FLH-QS-21 formulations showed reduced tumor development and greater overall survival. Hemocyanins, as opposed to QS-21, had no cytotoxic effect on either oral cancer cell line cultured in vitro, supporting the idea that the antitumor effects of hemocyanins are associated with their modulation of the immune response. Therefore, hemocyanin utilization would allow a lower QS-21 dosage to achieve therapeutic results. Overall, our study opens a new door to further investigation of the use of hemocyanins plus adjuvants for the development of immunotherapies against oral carcinoma.

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Section: Discussionmentioning

confidence: 99%

Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

Román

Campo

Villar

et al. 2019

Journal of Immunology Research

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“…The meat of R. venosa has relatively high protein, low fat content [118], and a high content of omega-3 essential fatty acids [119]. It contains proteins that can be used as food preservatives and antibiotics [120], vitamins [121], and antioxidants with anti-inflammatory, antitumor, and antiviral effects [122][123][124]. Rapana spp.…”

Section: Commercial Importance and Managementmentioning

confidence: 99%

Sustainable Management of High-Impact Non-Native Molluscs and Their Potential Commercial Importance in the Eastern Adriatic Sea

2023

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Molluscs, especially bivalves, play an important role in food production and are the dominant group in mariculture worldwide. They are also an important component of the marine ecosystem, influencing the food web and nutrient cycling, as well as creating and modifying habitats. In the Mediterranean Sea, about 578 non-native species have been reported, including 230 taxa of non-native mollusc species. In most regional European seas, including the Mediterranean, the socioeconomic impacts of high-impact mollusc species have generally been poorly studied, and only a few well-documented cases have been reported. The present study collects scientific information on non-native molluscs in Croatian waters that are classified as high impact according to the European Alien Species Information Network EASIN: one Gastropoda—Rapana venosa—and seven Bivalvia species—Anadara kagoshimensis, Anadara transversa, Arcuatula senhousia, Brachidontes pharaonis, Magallana gigas, Pinctada radiata, and Ruditapes philippinarum. This study aims to fill the gap in the literature on high-impact non-native molluscs in the eastern Adriatic Sea through reviewing the literature on examples from other European seas, focusing on the possibility of commercialization and sustainable management of high-impact non-native molluscs. The invasion of non-native molluscs must be managed appropriately, especially if commercialisation is chosen as a management strategy. Additional management measures must be selected and objectively evaluated, taking into account the specificities, location and feasibility of the proposed measures. The study could help researchers, decision-makers, and the public to address the problems with high-impact species in the Adriatic countries, but also in other regions where the monitoring of high-impact non-native species is still inadequate. In addition to Croatia, the monitoring and management of these species is also important for other countries in the eastern Adriatic: Slovenia, Montenegro and Albania.

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“…Rapana venosa ( Rv ) is an important marine snail which exhibits an increasing nutritional and economic importance [ 16 ]. Some studies have demonstrated that hemocyanin and peptides from Rv exhibited antimicrobial and antitumor activities [ 14 , 17 , 18 ]. Additionally, a previous study in our lab indicated that peptides from Rv could significantly improve inflammation damage in zebrafish induced by metronidazole.…”

Section: Introductionmentioning

confidence: 99%

The Peptide LLTRAGL Derived from Rapana venosa Exerts Protective Effect against Inflammatory Bowel Disease in Zebrafish Model by Regulating Multi-Pathways

Cao,

Xu,

Xia

et al. 2024

Marine Drugs

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Inflammatory bowel disease (IBD) is a chronic inflammatory bowel disease with unknown pathogenesis which has been gradually considered a public health challenge worldwide. Peptides derived from Rapana venosa have been shown to have an anti-inflammatory effect. In this study, peptide LLTRAGL derived from Rapana venosa was prepared by a solid phase synthesis technique. The protective effects of LLTRAGL were studied in a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced zebrafish colitis model. The underlying mechanisms of LLTRAGL were predicted and validated by transcriptome, real-time quantitative PCR assays and molecular docking. The results showed that LLTRAGL reduced the number of macrophages migrating to the intestine, enhanced the frequency and rate of intestinal peristalsis and improved intestinal inflammatory damage. Furthermore, transcriptome analysis indicated the key pathways (NOD-like receptor signal pathway and necroptosis pathway) that link the underlying protective effects of LLTRAGL’s molecular mechanisms. In addition, the related genes in these pathways exhibited different expressions after TNBS treatment. Finally, molecular docking techniques further verified the RNA-sequencing results. In summary, LLTRAGL exerted protective effects in the model of TNBS-induced colitis zebrafish. Our findings provide valuable information for the future application of LLTRAGL in IBD.

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Cytotoxic Effects of Rapana venosa Hemocyanin on Bladder Cancer Permanent Cell Lines

Cited by 4 publications

References 19 publications

Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

Sustainable Management of High-Impact Non-Native Molluscs and Their Potential Commercial Importance in the Eastern Adriatic Sea

The Peptide LLTRAGL Derived from Rapana venosa Exerts Protective Effect against Inflammatory Bowel Disease in Zebrafish Model by Regulating Multi-Pathways

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