Cytotoxic effects of polystyrene nanoplastics with different surface functionalization on human HepG2 cells

He, Yan; Li, Jing; Chen, Jiancheng; Miao, Xuhong; Li, Guo; He, Qiang; Xu, Haizhao; Li, Hong; Wei, Yanyan

doi:10.1016/j.scitotenv.2020.138180

Cited by 129 publications

(53 citation statements)

References 46 publications

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“…These data suggest that SaB-1 cells exposed to PS-NH 2 suffered oxidative stress. In this sense, reactive oxygen species (ROS) production is one of the more recognized effects of NPs on isolated cells [9,10,36,48] and has been registered in both mammalian [49][50][51][52] and fish cell lines [18,39,47]. Our data also showed that exposure to PS-NH 2 increases the transcription of pro-apoptotic (bax) genes and decreases that of anti-apoptotic (bcl2), what creates a balance favoring apoptosis.…”

Section: Exposure To Ps-nh 2 Produce Oxidative Stress and Apoptosis At Transcriptional Levelmentioning

confidence: 52%

Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

González-Fernández

Baños

Esteban

2021

IJMS

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Nanoplastics (NPs) are one of the most abundant environment-threatening nanomaterials on the market. The objective of this study was to determine in vitro if functionalized NPs are cytotoxic by themselves or increase the toxicity of metals. For that, we used 50 nm polystyrene nanoparticles with distinct surface functionalization (pristine, PS-Plain; carboxylic, PS-COOH; and amino PS-NH2) alone or combined with the metals arsenic (As) and methylmercury (MeHg), which possess an environmental risk to marine life. As test model, we chose a brain-derived cell line (SaB-1) from gilthead seabream (Sparus aurata), one of the most commercial fish species in the Mediterranean. First, only the PS-NH2 NPs were toxic to SaB-1 cells. NPs seem to be internalized into the cells but they showed little alteration in the transcription of genes related to oxidative stress (nrf2, cat, gr, gsta), cellular protection against metals (mta) or apoptosis (bcl2, bax). However, NPs, mainly PS-COOH and PS-NH2, significantly increased the toxicity of both metals. Since the coexistence of NPs and other pollutants in the aquatic environment is inevitable, our results reveal that the combined effect of NPs with the rest of pollutants deserves more attention.

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Section: Exposure To Ps-nh 2 Produce Oxidative Stress and Apoptosis At Transcriptional Levelmentioning

confidence: 52%

Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

González-Fernández

Baños

Esteban

2021

IJMS

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“…Nano- and microplastics, once regarded as inert particles without toxicity, are now seen as potentially harmful to organisms depending on exposure conditions and organism susceptibility [ 35 , 36 , 37 ]. The high surface area of plastic particles may be responsible for oxidative stress, cytotoxicity and particle translocation to other tissues, while their persistent nature limits their removal from the organism, leading to chronic inflammation [ 38 ]. As shown in the literature, nano- and microplastic particles can induce the production of ROS.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Properties of Polystyrene Nanoparticles with Different Diameters in Human Peripheral Blood Mononuclear Cells (In Vitro Study)

Kik

Bukowska

Krokosz

et al. 2021

IJMS

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With the ongoing commercialization, human exposure to plastic nanoparticles will dramatically increase, and evaluation of their potential toxicity is essential. There is an ongoing discussion on the human health effects induced by plastic particles. For this reason, in our work, we assessed the effect of polystyrene nanoparticles (PS-NPs) of various diameters (29, 44 and 72 nm) on selected parameters of oxidative stress and the viability of human peripheral blood mononuclear cells (PBMCs) in the in vitro system. Cells were incubated with PS-NPs for 24 h in the concentration range of 0.001 to 100 µg/mL and then labeled: formation of reactive oxygen species (ROS) (including hydroxyl radical), protein and lipid oxidation and cell viability. We showed that PS-NPs disturbed the redox balance in PBMCs. They increased ROS levels and induced lipid and protein oxidation, and, finally, the tested nanoparticles induced a decrease in PBMCs viability. The earliest changes in the PBMCs were observed in cells incubated with the smallest PS-NPs, at a concentration of 0.01 μg/mL. A comparison of the action of the studied nanoparticles showed that PS-NPs (29 nm) exhibited a stronger oxidative potential in PBMCs. We concluded that the toxicity and oxidative properties of the PS-NPs examined depended to significant degree on their diameter.

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“…PS-NH 2 NPs were more highly internalized in neonatal rat ventricular myocytes when compared with PS-COOH NPs, which resulted in decreased myocardial contractility (Roshanzadeh et al, 2021). PS-NH 2 NPs accumulated more than bare PS NPs in human hepatocellular carcinoma (HepG2) cells, and caused greater oxidative damage than PS-COOH NPs in HepG2 cells (He et al, 2020). PS-NH 2 NPs increased the cytotoxicity and induced cell apoptosis of human BEAS-2B (Chiu et al, 2015), Calu-3 (Paget et al, 2015), Caco-2 (Walczak et al, 2015;Busch et al, 2020), HT29-MTX-E12 (Inkielewicz-Stepniak et al, 2018), THP-1 cell lines (Fuchs et al, 2016;Hesler et al, 2019), and human alveolar cells (Roshanzadeh et al, 2020).…”

Section: Animals and Mammalian/human Cells In Vitromentioning

confidence: 99%

Influence of Functional Group Modification on the Toxicity of Nanoplastics

et al. 2022

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Nanoplastics (NPs) are ubiquitous in harvested organisms at various trophic levels, and more concerns on their diverse responses and wide species-dependent sensitivity are continuously increasing. However, systematic study on the toxic effects of NPs with different functional group modifications is still limited. In this review, we gathered and analyzed the toxic effects of NPs with different functional groups on microorganisms, plants, animals, and mammalian/human cells in vitro. The corresponding toxic mechanisms were also described. In general, most up-to-date relevant studies focus on amino (−NH2) or carboxyl (−COOH)-modified polystyrene (PS) NPs, while research on other materials and functional groups is lacking. Positively charged PS-NH2 NPs induced stronger toxicity than negatively charged PS-COOH. Plausible toxicity mechanisms mainly include membrane interaction and disruption, reactive oxygen species generation, and protein corona and eco-corona formations, and they were influenced by surface charges of NPs. The effects of NPs in the long-term exposure and in the real environment world also warrant further study.

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Cytotoxic effects of polystyrene nanoplastics with different surface functionalization on human HepG2 cells

Cited by 129 publications

References 46 publications

Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

Oxidative Properties of Polystyrene Nanoparticles with Different Diameters in Human Peripheral Blood Mononuclear Cells (In Vitro Study)

Influence of Functional Group Modification on the Toxicity of Nanoplastics

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