Cytotoxic effects of Pd(II) complexes on cancer and normal cells: Their DNA &amp; BSA adduct formation and theoretical approaches

Mandal, Saikat; Tarai, Swarup Kumar; Pan, Angana; Bhaduri, Rituparna; Biswas, Pritam; Moi, Sankar Ch.

doi:10.1016/j.bioorg.2022.106093

Cited by 7 publications

(7 citation statements)

References 73 publications

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“…It was found that the absorbance ratio ( A 260 / A 280 ) is in between 2.00 and 1.80, suggesting that the DNA is protein free. 30 The concentration was measured by considering the molar extinction coefficient 31 (ε) as 6600 M –1 cm –1 at 260 nm. The experiment was carried out by recording UV spectra of 20 μM RIBO or RIBO-TSC4X in the absence and presence of CT-DNA of various concentrations (20–120 μM).…”

Section: Methodsmentioning

confidence: 99%

Host–Guest Encapsulation of RIBO with TSC4X: Synthesis, Characterization, and Its Application by Physicochemical and Computational Investigations

et al. 2023

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In our present work, we synthesized a new encapsulated complex denoted as RIBO-TSC4X, which was derived from an important vitamin riboflavin (RIBO) and psulfonatothiacalix[4]arene(TSC4X). The synthesized complex RIBO-TSC4X was then characterized by utilizing several spectroscopic techniques such as 1 H-NMR, FT-IR, PXRD, SEM, and TGA. Job's plot has been employed to show the encapsulation of RIBO (guest) with TSC4X (host) having a 1:1 molar ratio. The molecular association constant of the complex entity (RIBO-TSC4X) was found to be 3116.29 ± 0.17 M −1 , suggesting the formation of a stable complex. The augment in aqueous solubility of the RIBO-TSC4X complex compared to pure RIBO was investigated by UV−vis spectroscopy, and it was viewed that the newly synthesized complex has almost 30 times enhanced solubility over pure RIBO. The enhancement of thermal stability upto 440 °C for the RIBO-TSC4X complex was examined by TG analysis. This research also forecasts RIBO's release behavior in the presence of CT-DNA, and at the same time, BSA binding study was also carried out. The synthesized RIBO-TSC4X complex exhibited comparatively better free radical scavenging activity, thereby minimizing oxidative injury of the cell as evident from a series of antioxidant and anti-lipid peroxidation assay. Furthermore, the RIBO-TSC4X complex showed peroxidase-like biomimetic activity, which is very useful for several enzyme catalyst reactions.

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Section: Methodsmentioning

confidence: 99%

Host–Guest Encapsulation of RIBO with TSC4X: Synthesis, Characterization, and Its Application by Physicochemical and Computational Investigations

et al. 2023

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“…The covered volume of the grid box was prepared to occupy the entire DNA molecule with a 40 × 40 × 80 number of points along the X × Y × Z direction with a 0.5 Å grid spacing. However, for the docking purpose with BSA, two tryptophan moieties (TRP134 and TRP213) were individually docked with Pt(II) complexes by the grid box of the same dimension around the tryptophan moiety only . The lowest-energy conformations were taken as the best docked poses.…”

Section: Methodsmentioning

confidence: 99%

“…BSA (bovine serum albumin) is the most abundant plasma protein present in the circulatory system, which plays a vital role for the distribution and transportation of metal ions, hormones, nutrients, and drug molecules to the specific organs or tissues in the body. The binding aptitude measurement of complexes with BSA was performed with the help of UV–vis spectroscopic titration and fluorescence emission quenching methods . A freshly prepared stock solution of BSA in Tris–HCl buffer was used for all the experiments.…”

Section: Methodsmentioning

confidence: 99%

“…However, for the docking purpose with BSA, two tryptophan moieties (TRP134 and TRP213) were individually docked with Pt(II) complexes by the grid box of the same dimension around the tryptophan moiety only. 31 The lowest-energy conformations were taken as the best docked poses.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…The binding aptitude measurement of complexes with BSA was performed with the help of UV−vis spectroscopic titration and fluorescence emission quenching methods. 31 A freshly prepared stock solution of BSA in Tris−HCl buffer was used for all the experiments. The UV−vis spectra of BSA (10 μM) after addition of complexes (10−100 μM) in BSA were recorded for calculation of binding constants and binding modes.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Brief Research on the Biophysical Study and Anticancer Behavior of Pt(II) Complexes: Their DNA/BSA Binding, Molecular Docking, and Cytotoxic Property

et al. 2022

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The potent bidentate carrier ligand 2-picolylamine (pic) has been used to synthesize Pt(II) complexes to know their bioactivity and anticancer property as reflected by PASS prediction software. The dichloro Pt(II) complex [Pt(pic)Cl 2 ], Pt-1, and its hydrolyzed diaqua complex [Pt(pic)(OH 2 ) 2 ] 2+ , Pt-2, were synthesized. The thiol-containing Pt(II) complexes [Pt(pic)(Lcys)] + , Pt-3, and [Pt(pic)(L-ac-L-cy)] + , Pt-4, were synthesized from Pt-2, which was obtained from hydrolysis of Pt-1. Their biomolecular interactions with BSA and DNA were executed by spectroscopic methods, and their cytototoxic property was tested by the MTT assay. In vitro biomolecular interactions of Pt(II) complexes with BSA and DNA were investigated by different spectroscopic and viscosity measurement methods for their pharmacokinetic and pharmacodynamic importance. The conformational change of BSA in the presence of a drug candidate was studied by Forster resonance energy transfer calculation and synchronous and three-dimensional fluorescence spectroscopic studies. A theoretical approach on optimization structures, highest occupied molecular orbital−lowest unoccupied molecular orbital energy, global reactivity parameters, time-dependent density functional theory, and molecular docking with BSA and DNA was executed to strengthen and support the experimental observations. In vitro cytotoxic profiles of the complexes like the anticancer activity and their level of reactive oxygen species production were brought under consideration on A549 cancer cells and the normal human embryonic kidney cell line HEK-293. The cytotoxic property was compared with that of the recognized anticancer drug cisplatin.

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Preliminary anticancer evaluation of new Pd(II) complexes bearing NNO donor ligands

Hussain,

Zafar

et al. 2024

Saudi Pharmaceutical Journal

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Cytotoxic effects of Pd(II) complexes on cancer and normal cells: Their DNA & BSA adduct formation and theoretical approaches

Cited by 7 publications

References 73 publications

Host–Guest Encapsulation of RIBO with TSC4X: Synthesis, Characterization, and Its Application by Physicochemical and Computational Investigations

Host–Guest Encapsulation of RIBO with TSC4X: Synthesis, Characterization, and Its Application by Physicochemical and Computational Investigations

Brief Research on the Biophysical Study and Anticancer Behavior of Pt(II) Complexes: Their DNA/BSA Binding, Molecular Docking, and Cytotoxic Property

Preliminary anticancer evaluation of new Pd(II) complexes bearing NNO donor ligands

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