Cytotoxic effects of neem oil in the midgut of the predator Ceraeochrysa claveri

Scudeler, Elton Luiz; Garcia, Ana Silvia Gimenes; Padovani, Carlos Roberto; Pinheiro, Patrícia Fernanda Felipe; Santos, Daniela Carvalho dos

doi:10.1016/j.micron.2015.10.005

Cited by 36 publications

(33 citation statements)

References 56 publications

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“…For example, the biocontrol activity of B. nigricans females was significantly impacted by the neurotoxic insecticides of emamectin benzoate and abamectin when used in tomatoes to control Tuta absoluta (Lepidoptera: Gelechiidae) (Biondi et al 2013). When treated with neem oil, changes in cocoon spinning and pronounced cytotoxic effects in the midgut of Ceraeochrysa claveri (Neuroptera: Chrysopidae), a common polyphagous predator in neotropical agroecosystems were observed (Scudeler and dos Santos 2013;Scudeler et al 2016). However, the impact of biopesticides on beneficial arthropods distributed in rice fields remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Selectivity and sublethal effects of some frequently-used biopesticides on the predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae)

Dai

Zhu

et al. 2019

Journal of Integrative Agriculture

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The green miridbug, Cyrtorhinus lividipennis, is an important predator of the rice brown planthopper, Nilaparvata lugens. In this study, the selective toxicity of seven commercial biopesticides for C. lividipennis was examined under laboratory conditions; abamectin was the most selective to C. lividipennis, followed by matrine and azadirachtin. Veratridine, rotenone, Bacillus thuringiensis and Beauveria bassiana showed less selectivity for C. lividipennis. Subsequently, matrine, abamectin and azadirachtin were selected for sublethal assessments with respect to C. lividipennis due to their high toxicities to N. lugens. C. lividipennis treated with sublethal concentrations (LC 10 and LC 20) of the three biopesticides could distinguish volatiles released from healthy and N. lugens-infested plants indicating that the biopesticides tested did not affect the foraging ability of surviving miridbugs. Azadirachtin decreased the consumption capability of C. lividipennis when the densities of N. lugens were 20, 30, 40 and 50 insects per vial. Sublethal concentration treatment did not impact the pre-oviposition period or egg hatchability of C. lividipennis. However, the fecundity of C. lividipennis exposed to azadirachtin and abamectin increased by 27-41% compared to the untreated individuals. In summary, abamectin or matrine together with C. lividipennis could be considered an effective, sustainable pest management strategy for rice.

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Section: Introductionmentioning

confidence: 99%

Selectivity and sublethal effects of some frequently-used biopesticides on the predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae)

Dai

Zhu

et al. 2019

Journal of Integrative Agriculture

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“…According to Silva de Moraes and Bowen (2000), Qi et al (2011), Scudeler and Santos (2013) and Scudeler et al (2016), the emergence of the vesicular rough endoplasmic reticulum is related with the dilation and fragmentation of the lamellar rough endoplasmic reticulum during the cell death process. In the present study, this vesiculation process might be occurring in the digestive cells of the individuals from group IV after the treatment.…”

Section: Discussionmentioning

confidence: 99%

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

et al. 2017

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The present study demonstrated the effects of dinotefuran (active ingredient of the acaricide Protetor Pet) on the ovary and midgut cells of semi engorged R. sanguineus females exposed to different concentrations of this chemical. For this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000ppm), groups III (6250ppm) and group IV (8334ppm of dinotefuran). All the ticks were immersed in the different concentrations of dinotefuran or in distilled water for 5min and then dried and kept in BOD incubator for 7days. The results showed alterations mainly regarding the damaged cell structures, such as yolk granules, organelles and the plasma membrane of the germ cells. In addition, structures related with defense mechanisms were found, such as vacuoles, cytoskeletal filaments, and myelin figures in the germ cells. Damages in the generative cells of the midgut, alterations in the size of digestive cells, the number of endosomes, digestive vacuoles, digestive residues, lipid drops and organelles in the cytoplasm of the digestive cells and the presence of microvilli in the plasma membrane of these cells also demonstrate the progressive damages caused by the action of dinotefuran in the midgut and germ cells of R. sanguineus semi-engorged females. The concentrations applied partially impaired the digestive processes; and, without proper nutrition, all the ectoparasite's physiologic events are prevented from occurring, leading the individual to death. The germ cells were also damaged, and probably would not be able to advance in their development (I-V) and complete the vitellogenesis, which would affect the fertility of the female and consequently impede the formation of a new individual.

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“…The evaluation of the side effects that may be associated with the direct or indirect exposure of natural enemies to biopesticides is important for the implementation of the correct tactics in IPM. In spite of being widely used, neem products are being assessed, and their compatibility with natural enemies is being questioned (Medina et al, 2003;Aggarwal and Brar, 2006;Cordeiro et al, 2010;El-Wakeil et al, 2013;Scudeler et al, , 2014Scudeler et al, , 2016a.…”

Section: Introductionmentioning

confidence: 99%

“…One important method that ecotoxicological studies have been using to investigate sublethal effects on natural enemies is via morphological studies of organs that are in contact way during the exposure to the biopesticides. Morphological and ultrastructural changes in this organ can provide information on delayed effects on non-target organisms (Malaspina and Silva-Zacarin, 2006;Scudeler et al, 2016a). Furthermore, this type of analysis contributes to the improved understanding of the mode of action of the biopesticide in the organism.…”

Section: Introductionmentioning

confidence: 99%

“…During exposure via ingestion, the midgut, which is responsible for digestive and absorptive functions, becomes an excellent target organ in ecotoxicological studies (Malaspina and Silva-Zacarin, 2006;Catae et al, 2014;Scudeler et al, 2016a). Cytotoxic effects on epithelial cells of the midgut of the Neotropical green lacewing Ceraeochrysa claveri (Navás, 1911) caused by treatment with neem oil have been described for all stages of development of this predator Scudeler et al, 2014;Scudeler et al, 2016a), but studies directed to the muscle layers that externally surrounds this organ are lacking.…”

Section: Introductionmentioning

confidence: 99%

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Neem oil ( Azadirachta indica A. Juss) affects the ultrastructure of the midgut muscle of Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

et al. 2017

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Cytomorphological changes, by means of ultrastructural analyses, have been used to determine the effects of the biopesticide neem oil on the muscle fibers of the midgut of the predator Ceraeochrysa claveri. Insects, throughout the larval period, were fed eggs of Diatraea saccharalis treated with neem oil at a concentration of 0.5%, 1% or 2%. In the adult stage, the midgut was collected from female insects at two stages of adulthood (newly emerged and at the start of oviposition) and processed for ultrastructural analyses. In the newly emerged insects obtained from neem oil treatments, muscle fibers showed a reduction of myofilaments as well as swollen mitochondria and an accumulation of membranous structures. Muscular fibers responded to those cellular injuries with the initiation of detoxification mechanisms, in which acid phosphatase activity was observed in large vesicles located at the periphery of the muscle fiber. At the start of oviposition in the neem oil treated insects, muscle fibers exhibited signs of degeneration, containing vacant areas in which contractile myofilaments were reduced or completely absent, and an accumulation of myelin structures, a dilatation of cisternae of sarcoplasmic reticulum, and mitochondrial swelling and cristolysis were observed. Enzymatic activity for acid phosphatase was present in large vesicles, indicating that mechanisms of lytic activity during the cell injury were utilized but insufficient for recovery from all the cellular damage. The results indicate that the visceral muscle layer is also the target of action of neem oil, and the cytotoxic effects observed may compromise the function of that organ.

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Cytotoxic effects of neem oil in the midgut of the predator Ceraeochrysa claveri

Cited by 36 publications

References 56 publications

Selectivity and sublethal effects of some frequently-used biopesticides on the predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae)

Selectivity and sublethal effects of some frequently-used biopesticides on the predator Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae)

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

Neem oil ( Azadirachta indica A. Juss) affects the ultrastructure of the midgut muscle of Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

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