Cytotoxic effects of MgO nanoparticles on human umbilical vein endothelial cells in vitro

Ge, Shengfang; Wang, Genhong; Shen, Yang; Zhang, Q; Jia, Dongyu; Wang, H; Dong, Qinglei; Yin, T. Y.

doi:10.1049/iet-nbt.2010.0022

Cited by 87 publications

(52 citation statements)

References 26 publications

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“…In previous studies, it was demonstrated that low concentrations (,200 µg/mL) of MgO and Se NPs have no cytotoxicity when examined by MTT assay. 13,19,22,23 In this regard, PaTu cells (1×10 5 cells/well) were exposed to logarithmically increasing concentrations of MgO NPs (0.1, 1, 10, and 100 µg/mL) and Se NPs (0.01, 0.1, 1, 10, and 100 µg/mL) at 37°C in a 5% CO 2 humidified atmosphere. After 24 hours of treatment, the cells were incubated to determine the viability using the MTT assay.…”

Section: Effect Of Mgo and Se Nps On Patu Cell Linementioning

confidence: 99%

Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

Shiri¹,

Navaei-Nigjeh²,

Baeeri³

et al. 2016

IJN

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Section: Effect Of Mgo and Se Nps On Patu Cell Linementioning

confidence: 99%

Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

Shiri¹,

Navaei-Nigjeh²,

Baeeri³

et al. 2016

IJN

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“…29 A systematic study of the mechanisms of interaction of nonfunctionalized CdTe and CdSe/ZnS QDs with a panel of live human cells of different origin has demonstrated that the nonfunctionalized QDs exploit the cell's active transport machineries for intranuclear delivery. 30 The effects of NPs such as carbon black, 31 silver, 32 metal oxides, 33 MgO, 34 SiO 2 , 35 QDs, 21 and polymeric NPs 19 on ECs have been studied under static conditions. However, no comprehensive study has been undertaken to evaluate the interaction of engineered NPs on human ECs under varying flow conditions.…”

mentioning

confidence: 99%

Multifactorial determinants that govern nanoparticle uptake by human endothelial cells under flow

Samuel¹,

Jain²,

O'Dowd³

et al. 2012

IJN

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Vascular endothelium is a potential target for therapeutic intervention in diverse pathological processes, including inflammation, atherosclerosis, and thrombosis. By virtue of their intravascular topography, endothelial cells are exposed to dynamically changing mechanical forces that are generated by blood flow. In the present study, we investigated the interactions of negatively charged 2.7 nm and 4.7 nm CdTe quantum dots and 50 nm silica particles with cultured endothelial cells under regulated shear stress (SS) conditions. Cultured cells within the engineered microfluidic channels were exposed to nanoparticles under static condition or under low, medium, and high SS rates (0.05, 0.1, and 0.5 Pa, respectively). Vascular inflammation and associated endothelial damage were simulated by treatment with tumor necrosis factor-α (TNF-α) or by compromising the cell membrane with the use of low Triton X-100 concentration. Our results demonstrate that SS is critical for nanoparticle uptake by endothelial cells. Maximal uptake was registered at the SS rate of 0.05 Pa. By contrast, endothelial exposure to mild detergents or TNF-α treatment had no significant effect on nanoparticle uptake. Atomic force microscopy demonstrated the increased formation of actin-based cytoskeletal structures, including stress fibers and membrane ruffles, which have been associated with nanoparticle endocytosis. In conclusion, the combinatorial effects of SS rates, vascular endothelial conditions, and nanoparticle physical and chemical properties must be taken into account for the successful design of nanoparticle-drug conjugates intended for parenteral delivery. Keywords: endothelium, shear stress, quantum dots, membrane ruffling, stress fibers, atomic force microscopy, microfluidics Nanoparticle (NP) technologies are significantly affecting the development of both therapeutic and diagnostic agents. Although enormous progress in the field of nanotechnology has been achieved, basic discoveries have not yet translated into effective targeted therapies. NPs can potentially improve the pharmacokinetics and pharmacodynamics of drugs; however, the complexity of in vivo systems imposes multiple barriers that severely inhibit efficiency, which must be overcome to fully exploit the theoretical potential of NPs. Endothelial cells (ECs) that line the interior of the entire vascular system represent a major barrier for therapeutic agents traveling from the bloodstream to the target tissues. Recent studies have focused on targeting the endothelium with NPs as therapeutic agents for a variety of pathological conditions in the vascular system because of the large population of ECs and their proximity to the blood flow.ECs in vivo are exposed to a variety of hemodynamic forces that are created by blood flow and by the pulse wave dictated by the cardiac cycle. Shear stress (SS) is the dragging mechanical force that acts at the interface between flowing blood and Dovepress submit your manuscript | www.dovepress.com the vessel wall. ECs recognize SS a...

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“…There is no data on toxicity and antileishmanial property of MgO NPs, but there are some studies on antibacterial activity of MgO NPs against certain Gram positive and negative bacteria (Lin et al, 2005). Previously study indicated that low concentrations of MgO NPs have no cytotoxicity, but their high concentrations have mild toxicity on human umbilical vein endothelial cells (Ge et al, 2011). The flow cytometry method shows that uncoated MgO NPs can generate reactive oxygen species, which induces apoptosis (Krishnamoorthy et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation

Jebali

Hekmatimoghaddam

Kazemi

et al. 2014

Drug and Chemical Toxicology

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The purpose of this research was to evaluate toxicity of uncoated magnesium oxide nanoparticles (MgO NPs), MgO NPs coated with Peanut agglutinin (PNA) lectin, and PNA alone on the promastigotes of Leishmania major (L. major) and macrophages of BALB/c mice. On the other hand, antileishmanial property of uncoated MgO NPs, lectin coated MgO NPs, and PNA lectin alone was evaluated, and also macrophage activation was investigated after treatment with these materials by measurement of nitrite, H2O2, and some interleukins. This study showed that PNA lectin and lectin coated MgO NPs had approximately no toxicity on L. major and macrophages, but some toxic effects were observed for uncoated MgO NPs, especially at concentration of 500 µg/mL. Interestingly, lectin coated MgO NPs had the highest antileishmanial activity and macrophage activation, compared with uncoated MgO NPs and PNA lectin.

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Cytotoxic effects of MgO nanoparticles on human umbilical vein endothelial cells in vitro

Cited by 87 publications

References 26 publications

Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

Multifactorial determinants that govern nanoparticle uptake by human endothelial cells under flow

Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation

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