“…Materials and Methods: Forty (40) ejaculates from six ( 6) boars, averaging eight ejaculates per boar, were collected with the gloved hand technique using a dummy sow. Six ( 6) different concentrations were selected for the in vitro study: 5,10,25,50,100,and 200 µM for As and Pb,and 5,10,25,50,100,and 200 mM for F. The ejaculates were co-incubated with heavy metals at these different concentrations and assessed after different incubation periods (0, 0.5, and 1 h) for sperm functional attributes, viz., sperm progressive motility, viability and membrane integrity, and sperm mitochondrial membrane potential (MMP). The combined effects of heavy metals on sperm functional attributes were also evaluated at different doses (5,10,25,50,100,5,10,25,50,100,and 5,10,25,50,100,and 200 μM/mM for Pb-F).…”