Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release

Kanjevac, Tatjana; Milovanović, Marija; Volarević, Vladislav; Lukić, Miodrag L.; Arsenijević, Nebojša; Marković, Dejan; Zdravković, Marija; Tešić, Živoslav; Lukić, Aleksandra

doi:10.2174/157340612799278351

Cited by 62 publications

(52 citation statements)

References 21 publications

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“…Cytotoxicity of phosphate cements has been attributed to the release of zinc ions and other chemical substances 22) . Similarly, it has been suggested that GICs, which released fluoride in high quantities, were highly toxic to human dental pulp stem cells 23) . Contrarily, Stanislawski et al reported that the concentration of F − , Sr 2+ and Al 3+ in the elutes of GICs was too low to be cytotoxic and suggested that the principal compounds responsible for cytotoxicity of RMGICs are unpolymerized resin monomers 2) .…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

et al. 2015

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The study aimed to 1) evaluate the cytotoxicity of luting cements: Hoffmann's Zinc Phosphate (Hoffmann's ZP), GC Fuji Plus Resin Modified Glass Ionomer (Fuji Plus RMGI) and 3M ESPE RelyX Unicem Resin Cement (RelyX Unicem RC) and 2) test if pre-washing reduces the cements' cytotoxicity. In vitro human gingival fibroblast (HGF) culture model was chosen. The cytotoxicity was evaluated by MTT test, the cell viability -by staining the cells with AO/EB dye mixture. The means±SD of Cell Survival Ratio (CSR%) were compared among different cement types under two testing conditions, with or without cement pre-washing. The CSR%s were compared by ANOVA and linear multiple regression (LMR). Hoffmann's ZPC was less cytotoxic, while Fuji Plus RMGIC and RelyX Unicem RC were more cytotoxic (ANOVA, p<0.001). The type of cement and cement pre-washing jointly explained 90% of cell survival (LMR, p<0.001, adjusted squared R=0.889). The commonly used luting cements such as Hoffmann's ZP, Fuji Plus RMGI and RelyX Unicem RC may have a cytotoxic potential.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

et al. 2015

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“…It has been reported that some dental luting cements are cytotoxic to the dental pulp cells and cause hypersensitivity in animal studies [7]. There have also been numerous biocompatibility studies of dental luting cement using cell culture techniques with various cells, such as gingival fibroblasts [8], dental pulp cells [9,10] and mouse fibroblasts [11]. However, all of the previous studies focused on the viability of the cells as the method of cytotoxicity evaluation for dental cements, method which have often been criticized due to the poor correlation with either animal studies or clinical results, as it does not reflect inflammatory mechanisms of the body system [12].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes

et al. 2015

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Dental luting cements are commonly used in dentistry for cementation of prosthetic restoration. Many previous studies focused on the measurement of the cell viability as the method of cytotoxicity evaluation during biocompatibility study for the material. In this study, the biocompatibility of various dental luting cements were evaluated using the new method of cytokine release measurement in order to better simulate inflammatory reactions in animal or clinical model using two different oral cells; immortalized human gingival fibroblast and immortalized human oral keratinocytes. Cells were exposed to extractions of various commercially available dental luting cements for different durations. Cytokines of IL-1α and IL-8 were measured from the supernatants of the cells and the results were then compared to the conventional MTT viability test. The result from the conventional cell viability study showed a relatively simple and straight forward indication that only one of the dental luting cements tested in this study was cytotoxic with increasing duration of exposure for both cells. Meanwhile, the result from the cytokine measurement study was much more complex at the time point they were measured, type of cells used for the study and the type of cytokines measured, all of which influenced the interpretation of the results. Hence, the better understanding of the cytokine release would be required for the application in biocompatibility evaluation.

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“…We speculated that this may be related to the cytotoxicity of fluoride. Many studies have shown that the effect of the high concentration of fluoride may cause cell apoptosis, in multiple cell types including hepatocytes [25], human embryo hepatocytes [26], human dental pulp stem cells [27], peripheral blood mononuclear cells [28], and osteoblasts [29]. Thus, we speculated that at higher concentrations of fluoride there may also be induction of B cell apoptosis.…”

Section: Discussionmentioning

confidence: 94%

Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro

Shi

Dong

et al. 2016

Cell Physiol Biochem

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Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.

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Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release

Abstract: Fuji Plus, Vitrebond and Fuji VIII, which released fluoride in higher quantities than other GICs, were highly toxic to human DPSCs. Opposite, low levels of released fluoride correlated to low cytotoxic effect of Composit, Fuji I and Fuji Triage.

Cited by 62 publications

References 21 publications

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes

Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro

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