Cytotoxic effects of bromelain in human gastrointestinal carcinoma cell lines (MKN45, KATO-III, HT29-5F12, and HT29-5M21)

Amini, Afshin; Ehteda, Anahid; Moghaddam, Samar Masoumi; Akhter, Javed; Pillai, Krishna; Morris, David L.

doi:10.2147/ott.s43072

Cited by 27 publications

(9 citation statements)

References 22 publications

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“…Ananas comosus L., the common pineapple, has been used medically for centuries by native inhabitants of Central and South America to treat a range of ailments, mainly digestive disorders, and heal wounds 1 , 2 . Its medicinal properties are attributed to bromelain, a mixture of proteases and non-protease components, including other enzymes (phosphatases, glucosidases, peroxidases, and cellulases), glycoproteins, and carbohydrates 3 .…”

Section: Introductionmentioning

confidence: 99%

In vitro performance of free and encapsulated bromelain

Ataide

Cefali

Figueiredo

et al. 2021

Sci Rep

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For centuries, bromelain has been used to treat a range of ailments, even though its mechanism of action is not fully understood. Its therapeutic benefits include enzymatic debridement of the necrotic tissues of ulcers and burn wounds, besides anti-inflammatory, anti-tumor, and antioxidant properties. However, the protease is unstable and susceptible to self-hydrolysis over time. To overcome the stability issues of bromelain, a previous study formulated chitosan-bromelain nanoparticles (C-B-NP). We evaluated the optimized nanoformulation for in vitro antioxidant, cell antiproliferative activities and cell migration/proliferation in the scratch assay, comparing it with free bromelain. The antioxidant activity of free bromelain was concentration and time-dependent; after encapsulation, the activity level dropped, probably due to the slow release of protein from the nanoparticles. In vitro antiproliferative activity was observed in six tumor cell lines for free protein after 48 h of treatment (glioma, breast, ovarian, prostate, colon adenocarcinoma and chronic myeloid leukemia), but not for keratinocyte cells, enabling its use as an active topical treatment. In turn, C-B-NP only inhibited one cell line (chronic myeloid leukemia) and required higher concentrations for inhibition. After 144 h treatment of glioma cells with C-B-NP, growth inhibition was equivalent to that promoted by the free protein. This last result confirmed the delayed-release kinetics of the optimized formulation and bromelain integrity. Finally, a scratch assay with keratinocyte cells showed that C-B-NP achieved more than 90% wound retraction after 24 h, compared to no retraction with the free bromelain. Therefore, nanoencapsulation of bromelain with chitosan conferred physical protection, delayed release, and wound retraction activity to the formulation, properties that favor topical formulations with a modified release. In addition, the promising results with the glioma cell line point to further studies of C-B-NP for anti-tumor treatments.

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Section: Introductionmentioning

confidence: 99%

In vitro performance of free and encapsulated bromelain

Ataide

Cefali

Figueiredo

et al. 2021

Sci Rep

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“… 2 , 7 - 9 Hence, we have developed a mucolytic that can solubilise the mucin in situ and enable the removal of mucinous material through a less invasive process such as a peritoneal catheter. 10 Further, the mucolytic agent has potent cytotoxicity that may enable its use as a cytotoxic agent 11 thereby facilitating the removal of peritoneal mucin with cytotoxic treatment in a single process.…”

Section: Introductionmentioning

confidence: 99%

Physical and chemical characteristics of mucin secreted by pseudomyxoma peritonei (PMP)

Pillai¹,

Akhter²,

Mekkawy³

et al. 2017

Int. J. Med. Sci.

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Background: Pseudomyxoma peritonei (PMP) is a rare disease with excess intraperitoneal mucin secretion. Treatment involves laparotomy, cytoreduction and chemotherapy that is very invasive with patients often acquiring numerous compromises. Hence a mucolytic comprising of bromelain and N-acetyl cystein has been developed to solubilise mucin in situ for removal by catherization. Owing to differences in mucin appearance and hardness, dissolution varies. Therefore the current study investigates the inter-mucin physical and chemical characteristics, in order to reformulate an effective mucolytic for all mucin.Method: PMP mucin, from the three categories (soft, semi hard and hard mucin) was solubilised and then various physical characteristics such as turbidity, density, kinematic viscosity were measured. The water content and the density of solid mucin were also determined. This was followed by the determination of sialic acid, glucose, lipid, Thiol (S-S and S-H) content of the samples. Lastly, the distribution of MUC2, MUC5B and MUC5AC was determined using western blot technique.Results: Both turbidity and kinematic viscosity and sialic acid content increased linearly as the hardness of mucin increased. However, density, hydration, protein, glucose, lipid and sulfhydryl and disulphide content decreased linearly as hardness of mucin increased. The distribution ratio of mucins (MUC2:MUC5B:MUC5AC) in soft mucin is 2.25:1.5:1.0, semi hard mucin is 1:1:1 and hard mucin is 3:2:1.Conclusion: The difference in texture and hardness of mucin may be due to cellular content, hydration, glucose, protein, lipids, thiol and MUC distribution. Soft mucin is solely made of glycoprotein whilst the others contained cellular materials.

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“…Consumption of pineapple and its components has been linked with immunity [ 5 – 18 ]. Some researchers also associated pineapple with cancer [ 19 – 23 ], wound healing [ 24 – 26 ], diabetes mellitus and liver disorders [ 27 – 31 ], diarrhea or other disorders of the gastrointestinal tract [ 32 , 33 ], and even tuberculosis [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Canned Pineapple Consumption on Nutritional Status, Immunomodulation, and Physical Health of Selected School Children

Cervo

Llido

Barrios

et al. 2014

Journal of Nutrition and Metabolism

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This randomized, controlled trial examined the effects of canned pineapple consumption on immunomodulation, nutritional status, and physical health of ninety-eight (98) school children with mean age of 8.44 ± 0.20. The study participants were divided into three groups: Group A (33) includes subjects who were not given canned pineapple, Group B (33) includes those who were given 140 g, and Group C (32) includes those given 280 g of canned pineapple for nine weeks. Each major group was further divided into two groups: normal (N) and underweight (U) based on 2007 WHO Growth Reference Standards. Sociodemographic, anthropometric, physical examination, dietary intake, hemoglobin level, and immunological data were analyzed. Results showed a decrease in incidence of viral and bacterial infections for both Group B and Group C (normal and underweight) after canned pineapple consumption. Granulocyte production increased by 0.77–26.61% for normal weight subjects and 14.95–34.55% for underweight. CD16+56 count augmented by 20.44–22.13% for normal weight and 3.57–15.89% for underweight subjects. Thus, intake of both one can (140 g) and two cans (280 g) of canned pineapple may shorten the duration and incidence of infection and may increase the production of granulocytes and CD16+56, but intake of two cans (280 g) demonstrated higher granulocyte and CD16+56 production. This trial is registered with Philippine Health Research Registry: PHRR140826-000225.

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Cytotoxic effects of bromelain in human gastrointestinal carcinoma cell lines (MKN45, KATO-III, HT29-5F12, and HT29-5M21)

Cited by 27 publications

References 22 publications

In vitro performance of free and encapsulated bromelain

In vitro performance of free and encapsulated bromelain

Physical and chemical characteristics of mucin secreted by pseudomyxoma peritonei (PMP)

Effects of Canned Pineapple Consumption on Nutritional Status, Immunomodulation, and Physical Health of Selected School Children

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