Cytotoxic Effects and Antibacterial Efficacy of a 3-Antibiotic Combination: An In Vitro Study

Chuensombat, Sorapong; Khemaleelakul, Saengusa; Chattipakorn, Siriporn; Srisuwan, Tanida

doi:10.1016/j.joen.2012.11.041

Cited by 97 publications

(122 citation statements)

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“…According to this, Ruparel et al 2) showed that TAP and DAP significantly reduced the survival of stem cells of the apical papilla (SCAPs) in a concentration dependent fashion, since lower concentrations of 0.01-0.10 mg/mL of TAP and DAP did not have an effect on SCAPs, while Chuensombat et al 17) determined that 0.39 mg/mL TAP was a safe dose, although it was unable to eradicate all bacteria. Recent recommendations suggest doses no higher than 1 mg/mL seeking for a balance between safety and antimicrobial effectivity 38,39) .…”

Section: Discussionmentioning

confidence: 99%

“…Other concerns associated with intracanal use of antibiotics are: fear of promoting antibiotic resistance, risk of allergic reaction 12) and biocompatibility of the medicament 16) . An excessive concentration of TAP might affect the host tissue, limiting tissue regeneration 17) . Therefore, the possibility of using chlorhexidine (CHX) or cetrimide (CTR) as intracanal medication in these regenerative procedures could be a safe alternative 18) .…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial efficacy of several intracanal medicaments for endodontic therapy

et al. 2017

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The antibacterial efficacy of intracanal medicaments for 2 and 7 days, in open apex root canals contaminated with Enterococcus faecalis biofilms was compared. One hundred and thirty-eight standardized uniradicular human roots were inoculated with E. faecalis. Colony forming units (CFU) were recorded before and after medication. Samples were divided in two (2/7 days), and subdivided (n=8/group): triple antibiotic paste (TAP); double antibiotic paste (DAP); dental base paste (DBP), consisting of calcium hydroxide, propylene glycol and calcium carbonate; DBP+0.2% chlorhexidine (CHX); DBP+0.2% cetrimide (CTR); DBP+0.2% CHX+0.2% CTR; 2.5% sodium hypochlorite (NaOCl); and distilled water. At both periods TAP, DAP, DBP+0.2% CHX, DBP+0.2% CTR and DBP+0.2% CHX+0.2% CTR exhibited no growth (NG) of E. faecalis. Distilled water and 2.5% NaOCl obtained the lowest CFU reduction. No growth with DBP was reached only at 7 days. CHX and/or CTR pastes were as effective as DAP and TAP. A 2-day period provided an effective disinfection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial efficacy of several intracanal medicaments for endodontic therapy

et al. 2017

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“…Interestingly, the direct incorporation of antibiotics to these membranes has given promising results in terms of inhibition of bacteria growth [7,17,18]. However, although the antibacterial effects of these drugs are well known, recent findings have warned of the potentially toxic effects of these highly concentrated antibiotic drugs on dental pulp stem cells and dental pulp fibroblasts [19,20]. Furthermore, the direct drug release from polymeric substrates undergoes bulk degradation, modifying dramatically the physico-chemical properties of the device, mainly in terms of mechanical properties [21].…”

Section: Introductionmentioning

confidence: 99%

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

et al. 2015

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Article:Gentile, P., Frongia, M.E., Redon, M.C. et al. (4 more authors) (2015) Functionalised nanoscale coatings using Layer-by-Layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces. Acta Biomaterialia.https://doi.org/10.1016/j.actbio.2015.04.009 eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. AbstractIn order to achieve high local biological activity and reduce the risk of side effects of antibiotics in the treatment of periodontal and bone infections, a localised and temporally controlled delivery system is desirable. The aim of this research was to develop a functionalised and resorbable surface to contact soft tissues to improve the antibacterial behaviour during the first week after its implantation in the treatment of periodontal and bone infections. Solvent-cast poly(D,L-lactide-coglycolide acid) (PLGA) films were aminolysed and then modified by Layer-by-Layer technique to obtain a nano-layered coating using poly(sodium4-styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) as polyelectrolytes. The water-soluble antibiotic, metronidazole (MET), was incorporated from the ninth layer. Infrared spectroscopy showed that the PSS and PAH absorption bands increased with the layer number. The contact angle values had a regular alternate behaviour from the ninth layer. X-Ray Photoelectron Spectroscopy evidenced two distinct peaks, N 1s and S 2p , indicating PAH and PSS had been introduced. Atomic Force Microscopy showed the presence of polyelectrolytes on the surface with a measured roughness about 10 nm after 20 layers deposition.The drug release was monitored by Ultraviolet-visible spectroscopy showing 80% loaded-drug delivery in 14 days. Finally, the biocompatibility was evaluated in vitro with L929 mouse fibroblasts and the antibacterial properties were demonstrated successfully against the keystone periodontal bacteria Porphyromonas gingivalis, which has an influence on implant failure, without compromising in vitro biocompatibility. In this study, PLGA was successfully modified to obtain a localised and temporally controlled drug delivery system, demonstrating the potential value of LbL as a coating technology for the manufacture of me...

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“…A quebra dos íons de hidrogênio do grupo HCl resulta em condição ácida que é uma condição desfavorável para a viabilidade celular (Kobayashi et al, 2007 (Holland et al, 1999;Dummer, 2011), o que é muito controverso entre os autores, uma vez que alguns autores o consideram biocompatível (Ji et al, 2010;Gomes-Filho et al, 2012;Ruparel et al, 2012;Althumairy et al, 2014). (Ruparel et al, 2012;Chuensombat et al, 2013;Althumairy et al, 2014;Phumpatrakom;Srisuwan, 2014 (Hahn et al, 1991). O LTA é uma molécula anfifílica ancorada à parede celular bacteriana e está associado a processos inflamatórios.…”

Section: Discussionunclassified

“…Há estudos in vitro que avaliam a citotoxicidade de substâncias sobre células de papila analisando o efeito das medicações sobre células em condição de homeostasia (Ruparel et al, 2012;Chuensombat et al, 2013). Entretanto, em quadros de periodontite apical em dentes com rizogênese incompleta, as células da papila que eventualmente tenham sobrevivido se encontram em um ambiente pró-inflamatório resultante da presença de microrganismos na cavidade pulpar e de mediadores inflamatórios liberados por células da polpa, da própria papila ou dos tecidos periapicais.…”

Section: Introductionunclassified

Influência de medicações intracanal utilizadas em procedimentos endodônticos regenerativos na sobrevivência de células da papila apical in vitro

Oliveira¹

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Oliveira AP. Influence of intracanal medications used in regenerative endodontic procedures on survival of apical papilla cells in vitro [thesis]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2014. Versão Corrigida.Regenerative Endodontic procedures have provided changes in treatment of patients with immature teeth and apical periodontitis enabling full root development and lower incidence of dental fracture. Intracanal dressings are used for disinfection; however, their effect on apical papilla cells is poorly elucidated. Additionally, the effect of these substances on cells previously subjected to proinflammatory condition is still unknown. The aim of this study was to evaluate the cytotoxicity of intracanal dressings used in Endodontics regenerative procedures on cultured human apical papilla cells at physiologic and activated condition. Cell culture was established from the apical papilla removed from an extracted immature third molar. The substances studied were triple antibiotic modified paste: ciprofloxacin, metronidazole and cephalosporin (1:1:1); CFC: ciprofloxacin, metronidazole and calcium hydroxide (1:1:2) and modified CFC: ciprofloxacin , metronidazole and calcium hydroxide (2:2:1). Part of the cells was stimulated previously with lipotheichoic acid (LTA) of Enterococcus faecalis por 7 days. Once plated, cells were exposed to increasing concentration of the medications for 1, 3, 5 and 7 days. Cell viability was evaluated by means of 3-bromide (4.5-dimetiliazol-2-yl) -2.5-difeniltetraze (MTT) and Nitric Oxide (NO) release was assessed by the Griess method. The statistical analysis was done through analysis of variance with 1 criteria (ANOVA) followed by Tukey test with 5% of significance level. Modified CFC was the medication that demonstrated the less cytotoxic effect on cell viabilityat the experimental periods studied while CFC promoted significant decrease on cell viability specially after 7 days of contact. The modified triple antibiotic paste resulted in important alteration of cell viability being considered the most citotoxic. Cellular activation by LTA resulted in increased levels of mitochondrial activity for all medications being more evident at the longer experimental periods. Cellular activation also contributed to higher levels of nitric oxide release. In conclusion, the cytotoxic effect of the tested medications is dependent on concentration, time of contact and cellular condition, being the triple antibiotic modified paste the most cytotoxic in high concentrations leading clinically in the decreased of the cells viability of the apical papilla, decreasing the success of regenerative procedures.

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Cytotoxic Effects and Antibacterial Efficacy of a 3-Antibiotic Combination: An In Vitro Study

Cited by 97 publications

References 30 publications

Antibacterial efficacy of several intracanal medicaments for endodontic therapy

Antibacterial efficacy of several intracanal medicaments for endodontic therapy

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

Influência de medicações intracanal utilizadas em procedimentos endodônticos regenerativos na sobrevivência de células da papila apical in vitro

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