Cytotoxic effect of sodium arsenite on Nile tilapia ( Oreochromis niloticus ) in vivo

Self Cite

The objectives of the present study were to determine the concentrations of heavy metals and metalloids in water, sediment and Osteochilus vittatus fish, and to assess chromosome aberrations, serum biochemical changes and histopathological alterations in O. vittatus from the Nam Kok river near the Sepon gold-copper mine, Lao People’s Democratic Republic compared with the reference area. The results showed that Fe, Mn and Ni in water, As and Cd in sediment as well as As, Cd, Cr, Mn and Ni in O. vittatus muscle samples near the gold-copper mine exceeded standard values. Furthermore, the chromosome assessment in O. vittatus revealed seven types of chromosome aberrations, and the highest total number of chromosome aberrations was a centromere gap. The total number of chromosome aberrations, cell number with chromosome aberrations and percentage of chromosome aberrations in O. vittatus as well as serum liver enzymes between the studied areas were significantly different (p < 0.05). The liver histopathological alterations of the fish near the gold-copper mine revealed atypical cellular structures as nuclear membrane degeneration, rough endoplasmic reticulum disintegration and abnormal cytoplasmic mitochondria. The results of this study suggested that heavy metal and metalloid contaminations from the Sepon gold-copper mine area negatively affect O. vittatus fish in terms of chromosomal defects, serum biochemical changes and liver histopathological appearances.

Section: Discussionmentioning

confidence: 99%

Effects of Contamination by Heavy Metals and Metalloids on Chromosomes, Serum Biochemistry and Histopathology of the Bonylip Barb Fish Near Sepon Gold-Copper Mine, Lao PDR

Soulivongsa

Tengjaroenkul

Neeratanaphan

2020

Self Cite

Section: Discussionmentioning

confidence: 99%

“…The Nam Kok River, located near a Sepon gold-copper mine in the Viraboury District, Savannakhet Province, Lao People's Democratic Republic, at GPS location 16 • 54.78 E, near agricultural and domestic areas without mining and electronic activities, i.e., potential load for naturally leached elements, was set as a control area (Figure 1).…”

Section: Study Areamentioning

confidence: 99%

“…Khamlerd et al [46] reported that Channa striata affected by heavy metals (As, Pb, Cd, Cr, Cu, Fe, Zn, Mn and Ni) from the Bueng Jode reservoir in Thailand showed seven types of chromosome abnormalities. Sriuttha et al [50] and Tengjaroenkul et al [54] demonstrated six types and five types of chromosome abnormalities in O. niloticus in a domestic wastewater canal and in an in vitro experiment, respectively. Neeratanaphan et al [16] conducted a cytotoxic assessment of Esomus metallicus from a gold mine area in the Wang Saphung district of Loei Province in Thailand and found six types of chromosome abnormalities.…”

Section: Chromosome Study In H Macrolepidotamentioning

confidence: 99%

See 1 more Smart Citation

Cytogenetic, Serum Liver Enzymes and Liver Cell Pathology of the Hampala Barb Fish (Hampala macrolepidota) Affected by Toxic Elements in the Contaminated Nam Kok River near the Sepon Gold-Copper Mine, Lao PDR

Soulivongsa

Tengjaroenkul

Patawang

et al. 2021

Self Cite

This study aimed to determine toxic element concentrations in aquatic environments, including water and sediment, and in the Hampala macrolepidota fish, and to evaluate chromosome abnormalities, serum liver enzyme changes and liver histopathological alterations in H. macrolepidota from the Nam Kok River near the Sepon gold–copper mine, Lao People’s Democratic Republic, as compared with a control area without mining activity. The results revealed significant differences (p < 0.05) in As, Ba, Cu, Fe, Mn, Ni, Se and Zn in water, in all of the studied potentially toxic elements in sediment, and in As, Ba, Cd, Cr, Cu, Mn, Ni, Se, and Zn in the fish between the study and control areas. A chromosome assessment demonstrated 6 types of chromosome abnormalities, among which centric gap had the highest total number of chromosome abnormalities. Percentage of chromosome abnormalities, percentage of cells with chromosome abnormalities and serum liver enzymes in H. macrolepidota were significantly different (p < 0.05) between the two studied areas and were higher in the contaminated fish than in the control fish. The observation of liver histopathological changes revealed cellular degeneration, such as nuclear damage, abnormal cytoplasmic mitochondria and the disintegration of rough endoplasmic reticulum. The results indicate that the contamination of potentially toxic elements in the Nam Kok River near the Sepon gold–copper mine area negatively affected chromosomes, serum liver enzymes and liver cell structures in H. macrolepidota.

“…The mixtures of extracted DNA molecules from the fish gills and livers were successfully amplified by 25 inter-simple sequence repeat (ISSR) primers (Table 2) in a PCR cycler (Flex Cycler 2 , Analytikjena) [27]. Each DNA band was evaluated and recorded as the following diallelic characters: present = 1 and absent = 0.…”

Section: Genotoxicity Studymentioning

confidence: 99%

Genotoxicity and Oxidative Stress in Experimental Hybrid Catfish Exposed to Heavy Metals in a Municipal Landfill Reservoir

Neeratanaphan

Kamollerd

Suwannathada

et al. 2020

Self Cite

This study aimed to investigate the concentrations of Cr, Cd and Pb in the water, sediment and experimental hybrid catfish muscles, and to compare the genetic differentiation and the levels of oxidative stress biomarkers (malondialdehyde and protein carbonyl) between the catfish from the contaminated reservoir near a municipal landfill and the reference area after chronic exposure. The concentrations of all metals in the water and the concentration of Cd in the sediment exceeded Thailand’s surface water quality and soil quality standards, respectively, whereas the concentrations of these metals in fish muscles did not exceed Thailand’s food quality standards. Dendrogram results in terms of genetic similarity values of the catfish from the reference and the landfill areas were 0.90 to 0.96 and 0.79 to 0.86, respectively, implying that the genetic differentiation of the fish from the landfill was greater than of those from the reference area. The fish in the landfill reservoir had slightly increased protein carbonyl levels. The results indicate that chronic heavy metal exposure can cause genotoxicity of the hybrid catfish and induce protein carbonyl as an oxidative stress biomarker in the reservoir near a municipal landfill.