Cytotoxic effect of methanol extract of &lt;i&gt;Conyza bonariensis&lt;/i&gt; on DMBA-induced skin carcinogenesis: An &lt;i&gt;in vivo&lt;/i&gt; study

Saleem, Mohammad; Naseer, Faiza; Hussain, Khalid; Alamgeer, -

doi:10.3329/bjp.v10i2.23063

Cited by 6 publications

(6 citation statements)

References 32 publications

(34 reference statements)

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“…The catalase activity was determined by the method described by Saleem et al, (2015) 17. Briefly, to the tissue supernatant (50 µl), 1.0 ml of 50 mM phosphate buffer (pH 7) and 0.1 ml of 30 mM hydrogen peroxide were added.…”

Section: Methodsmentioning

confidence: 99%

Chemomodulatory Potential of Bartogenic Acid Against DMBA/Croton Oil Induced Two-Step Skin Carcinogenesis in Mice

et al. 2016

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Barringtonia racemosa fruits are believed to be useful in cancer treatment in Ayurveda, the Indian system of medicine. In present study, bartogenic acid (BA), a triterpenoid constituent of Barringtonia fruits was evaluated for its cytotoxicity property using the human skin carcinoma cell line (SCC-13) and human peripheral blood mononuclear cells (PBMC). The chemopreventive efficacy of BA was evaluated against the DMBA/Croton oil-induced skin carcinogenesis in mice.BA was orally administered at the doses of 1, 2 or 4 mg/kg/day or applied topically every day for 12 weeks following DMBA application. The in vitro data from cell lines revealed that BA induces cytotoxicity against the SCC-13 cells (IC50=7.5 µM). It was found 4.05 times more selective to exert cytotoxicity against SCC-13 as compared to the PBMC (IC50=30.4 µM). The in vivo datacollected from mice model of DMBA/Croton oil-induced skin carcinogenesis revealed that BA administered orally or applied topically, both reduced the precancerous skin lesions andthe incidence of tumor bearing. The oral doses of BA (2 and 4 mg/kg) and topical treatment significantly reduced the incidence and number of skin papillomas. At these doses, BA also increased the activities of catalase and superoxide dismutase and induced an increase in glutathionecontent and inhibited lipid peroxidation in the skin. These findings reveal the chemopreventive efficacy of BA and also demonstrate that it contributes to the cytotoxic and antioxidative effects of Barringtonia racemosa fruits. The study also validates the traditional claims of Barringtonia fruits and provides a scientific basis of its chemopreventive property.

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Section: Methodsmentioning

confidence: 99%

Chemomodulatory Potential of Bartogenic Acid Against DMBA/Croton Oil Induced Two-Step Skin Carcinogenesis in Mice

et al. 2016

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“…[45][46][47][48][49][50][51] In fact, promising results were obtained with the methanolic extract of C. bonariensis from Pakistan, as it demonstrated to be active in DMBA-induced skin carcinogenesis in vivo studies. 52 Reasonable comparisons with previous studies could not be made for many reasons. We have summarized these studies in Table 9, with short comments added for clarifications.…”

Section: S160mentioning

confidence: 97%

Antimicrobial Screening of Medicinal Plants Popularly used in Mato Grosso for Treating Infections: Advances on the Evaluation of Conyza bonariensis (L.) Cronquist in vitro and in vivo Antibacterial Activities

Paula¹,

Martins²,

Arunachalam³

et al. 2018

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infections and resistance to antifungal agents have increased significantly in immunocompromised patients and these infections are responsible for a high rate of morbidity/mortality in severe cases. 1,4 The growing need for more effective and safe antimicrobial agents has led to the renewal of multidisciplinary investigation on natural products, where new approaches combined with traditional techniques, are accelerating tracking of substances, which present antimicrobial activities. In addition, it has also allowed identification of the molecular targets responsible for their effects, moreover, many of these substances present new mechanisms of action. 4 Medicinal plants constitute an arsenal of chemicals

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“…Extract The SCC13 cells were procured from ATCC (Manassas, VA), and human PBMC was isolated by density centrifugation of heparinized blood of healthy donors using the dextran T-500 sedimentation method (Patil et al 2016). The cells were grown adherently in RPMI-1640 media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin at 37°C in 5% CO2 with 95% air (Saleem et al 2015).…”

Section: In Vitro Cytotoxic Efficacy Of Cb N-hexanementioning

confidence: 99%

Chemomodulatory Potential of n-Hexane Extract of Conyza bonariensis Against Skin Carcinogenesis in Mice Induced by Carcinogens: An in Vitro and in Vivo Study

Naseer,

Imran Aziz,

Asghar

et al. 2022

Phytopharmacological Com.

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Conyza bonariensis (CB) is a cosmopolitan herb, family Asteraceae (Compositeae), commonly known as mrich booti or gulava previously used to cure cancer in traditional medicine. In the present study, n-hexane fraction by CB's triterpenoid constituents was assessed for their cytotoxic property using the human skin carcinoma cell line (SCC-13) and human peripheral blood mononuclear cells (PBMC). The chemo modulatory potential of CB n-hexane was evaluated against skin carcinogenesis in mice induced by (7-12-dimethylbenz(a)antheracene) DMBA and Croton oil. CB n-hexane extract was orally administered at the doses of 3, 6, and 9 mg/kg/day, and 9 mg/kg was also applied locally on mice skin for 16 weeks. The results from cell lines revealed that CB induces cytotoxicity against the SCC-13 cells (IC50=7.5 µM). It was found to be 4.05 times more selective in exerting cytotoxicity against SCC-13 than the PBMC (IC50=30.4 µM). The results from the mice model of skin carcinogenesis have shown that whole plant n-hexane extract administered via oral and topical routes, both reduced the number of precancerous skin papillomas and their incidence. At these concentrations, CB n-hexane also augmented the actions of catalase and superoxide dismutase, glutathione content and reduced the skin lipid peroxidation levels. These outcomes presented the cytotoxic and antioxidative effects of CB n-hexane whole plant extract and also authenticates the traditional assertions and strengthen the technical base of its chemopreventive activity.

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Cytotoxic effect of methanol extract of <i>Conyza bonariensis</i> on DMBA-induced skin carcinogenesis: An <i>in vivo</i> study

Cited by 6 publications

References 32 publications

Chemomodulatory Potential of Bartogenic Acid Against DMBA/Croton Oil Induced Two-Step Skin Carcinogenesis in Mice

Chemomodulatory Potential of Bartogenic Acid Against DMBA/Croton Oil Induced Two-Step Skin Carcinogenesis in Mice

Antimicrobial Screening of Medicinal Plants Popularly used in Mato Grosso for Treating Infections: Advances on the Evaluation of Conyza bonariensis (L.) Cronquist in vitro and in vivo Antibacterial Activities

Chemomodulatory Potential of n-Hexane Extract of Conyza bonariensis Against Skin Carcinogenesis in Mice Induced by Carcinogens: An in Vitro and in Vivo Study

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