Cytotoxic effect of &lt;em&gt;Drimia maritima&lt;/em&gt; bulb extract and induction of mitochondrial apoptotic signaling in human breast cancer cells, MCF-7 and MDA-MB-468

Hamzeloo-Moghadam, Maryam; Aghaei, Mahmoud; Abdolmohammadi, Mohammad Hossein; Khalaj, Amir; Fallahian, Faranak

doi:10.2147/ott.s182786

Cited by 14 publications

(5 citation statements)

References 29 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, D. maritima induced cell death in human breast cancer cells via the intrinsic apoptotic pathway [ 119 ]. Baccatin III promoted apoptosis in JR4-Jurkat cells with possible participation of anti-apoptotic Bcl-2 and mitochondrial membrane damage [ 120 ]. Furthermore, limonin glucoside induced apoptosis through a reduction in the transcription ratio of Bcl-2/Bax and initiating the release of cytochrome c from mitochondria to cytosol, which confirm the activation of the intrinsic apoptotic pathway in SW480 colon cancer cells [ 121 ].…”

Section: Resultsmentioning

confidence: 99%

Selective in vitro cytotoxicity effect of Drimia calcarata bulb extracts against p53 mutant HT-29 and p53 wild-type Caco-2 colorectal cancer cells through STAT5B regulation

2021

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Selective in vitro cytotoxicity effect of Drimia calcarata bulb extracts against p53 mutant HT-29 and p53 wild-type Caco-2 colorectal cancer cells through STAT5B regulation

2021

View full text Add to dashboard Cite

show abstract

“…For example, Hamzeloo-Moghadam reported that the IC50 values for the non-hemolytic ethanol extract of U. maritima against breast cancer MCF7 cells were 11.01 µg/mL. Additionally, IC50 values for the non-hemolytic acetone extract of U. maritima against breast cancer MCF7 cells was 6.01 µg/mL, while the Doxorubicin drug (positive control) exhibited a cytotoxic effect with a higher IC50 value (52.35 µg/mL) [ 19 ]. Another example is the study conducted by Obeidat and Sharan, which found that the effective doses of D. maritima that inhibited 50% of growth (IC50) of MCF-7 and MDA-MB-468 cells were 20.48 ± 1.17 µg/mL and 25.74 ± 2.05 µg/mL, respectively.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis and Anticancer Properties of Drimia maritima Bulb Extracts on Colorectal Cancer Cells

et al. 2023

View full text Add to dashboard Cite

Cancer is a worldwide health problem and is the second leading cause of death after heart disease. Due to the high cost and severe side effects associated with chemotherapy treatments, natural products with anticancer therapeutic potential may play a promising role in anticancer therapy. The purpose of this study was to investigate the cytotoxic and apoptotic characteristics of the aqueous Drimia maritima bulb extract on Caco-2 and COLO-205 colorectal cancer cells. In order to reach such a purpose, the chemical composition was examined using the GC-MS method, and the selective antiproliferative effect was determined in colon cancer cell lines in normal gingival fibroblasts. The intracellular ROS, mitochondrial membrane potential, and gene expression changes in selected genes (CASP8, TNF-α, and IL-6 genes) were assessed to determine the molecular mechanism of the antitumor effect of the extract. GC-MS results revealed the presence of fifty-seven compounds, and Proscillaridin A was the predominant secondary metabolite in the extract. The IC50 of D. maritima bulb extract on Caco-2, COLO-205, and the normal human gingival fibroblasts were obtained at 0.9 µg/mL, 2.3 µg/mL, and 13.1 µg/mL, respectively. The apoptotic effect assay indicated that the bulb extract induced apoptosis in both colon cancer cell lines. D. maritima bulb extract was only able to induce statistically significant ROS levels in COLO-205 cells in a dose-dependent manner. The mitochondrial membrane potential (MMP) revealed a significant decrease in the MMP of Caco-2 and COLO-205 to various concentrations of the bulb extract. At the molecular level, RT-qPCR was used to assess gene expression of CASP8, TNF-α, and IL-6 genes in Caco-2 and COLO-205 cancer cells. The results showed that the expression of pro-inflammatory genes TNF-α and IL-6 were upregulated. The apoptotic initiator gene CASP8 was also upregulated in the Caco-2 cell line and did not reach significance in COLO-205 cells. These results lead to the conclusion that D. maritima extract induced cell death in both cell lines and may have the potential to be used in CRC therapy in the future.

show abstract

“…Considering annexin V’s high affinity to PS binding, it was used to identify early apoptotic rate. PI, a red fluorescent intercalating dye, was used as a DNA stain to study dead cells or late apoptotic rate ( Hamzeloo-Moghadam et al ., 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Differential apoptotic gene expression in the male partners of infertile couples with normal and abnormal sperm parameters

Afsari¹,

Talebi²,

Tafti³

et al. 2022

JBRA

View full text Add to dashboard Cite

Objective The purpose of this study was to investigate the cellular and molecular levels of apoptosis induction in three groups of male partners of infertile couples, one featuring subjects with normal sperm parameters and unexplained male infertility (UMI), one including men with abnormal sperm parameters, and one with fertile men as controls. Methods Twenty-five infertile men with abnormal sperm parameters and 25 men with UMI and normal sperm parameters were recruited as experimental group I and experimental group II; 25 fertile men were included as controls. The mRNA levels of Fas, Fas ligand, Caspase 8, Bax , and Bcl2 were measured in the three groups. The cellular rates of early and late apoptosis were assessed using annexin V and propidium iodide staining. Results The expression of Bax, Bcl2 , and the Bax/Bcl2 ratio in experimental group I was significantly higher than that in experimental group II and controls. However, the Bax/Bcl2 ratio was less than 1 among all groups. No significant difference was found among study groups regarding the gene expression of Fas, Fas ligand , and Caspase 8 . No significant difference was seen in early apoptotic rates of sperm among study groups. The highest number of necrotic sperm cells was detected in experimental group I. Conclusions The findings showed that the external pathways of apoptosis were not activated in the absence of external stimuli of sperm apoptosis in ejaculated sperm. Regardless of fertility status, apoptosis gene induction in the internal pathway was associated with abnormalities in sperm motility and/or morphology in men with abnormal parameters.

show abstract

Cytotoxic effect of <em>Drimia maritima</em> bulb extract and induction of mitochondrial apoptotic signaling in human breast cancer cells, MCF-7 and MDA-MB-468

Cited by 14 publications

References 29 publications

Selective in vitro cytotoxicity effect of Drimia calcarata bulb extracts against p53 mutant HT-29 and p53 wild-type Caco-2 colorectal cancer cells through STAT5B regulation

Selective in vitro cytotoxicity effect of Drimia calcarata bulb extracts against p53 mutant HT-29 and p53 wild-type Caco-2 colorectal cancer cells through STAT5B regulation

Phytochemical Analysis and Anticancer Properties of Drimia maritima Bulb Extracts on Colorectal Cancer Cells

Differential apoptotic gene expression in the male partners of infertile couples with normal and abnormal sperm parameters

Contact Info

Product

Resources

About