Cytotoxic effect of achatininH (lectin) from Achatina fulica against a human mammary carcinoma cell line (MCF7)

Abstract

The hemolymph-derived achatinin(H) (lectin) from Achatina fulica showed a marked cytotoxic effect on MCF7, a human mammary carcinoma cell line. IC(50) values as measured by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay for achatinin(H) ranged from 6 to 10 microg/ml in the MCF7 cells. MCF7 cells showed significant morphological changes leading to cell death. The above cell death was observed after 48 h of treatment with 8 microg/ml when compared to untreated cells. Alterations in the tumor marker enzymes, as well as in antioxidant enzymes, were observed after achatinin(H) treatment. The specificity and purity of the achatinin(H) was confirmed by the Western blot assay. Achatinin(H) binding to MCF7 cells was detected by anti-achatinin(H), and visualization of the achatinin(H) binding sites on confluent MCF7 cells was confirmed by flourescein isothiocyanate conjugated secondary antibody. MCF7-treated cells fluoresced, indicating the presence of achatinin(H) binding sites. Fluorescence-activated cell sorting analysis of the cell cycle showed a significant increase in S-phase in MCF7 cells after 48 h of achatinin(H) treatment. The cells were arrested in G(2)/M phase of the cell cycle after 48 h with significant changes in cell viability. Cellular damage was confirmed by agarose gel electrophoresis with the characteristic appearance of a DNA streak in treated MCF7 cells indicating the ongoing apoptosis.

Hemolymph of molluscan origin: from biochemistry to modern biomaterials science

“…Such terrestrial gastropod species as Achatina fulica (Bowdich, 1822), Cornu aspersum (ex. Helix aspersa) (Müller, 1774) and the aquatic mollusk species as Rapana venosa (Valenciennes, 1846), Megathura crenulata (Sowerby, 1825) are only a few selected examples from which hemolymph is used as a source of such biologically active substances [25][26][27][28][29][30][31][32][33].…”

Rhizoctonia Bataticola Lectin (RBL) Induces Caspase-8-Mediated Apoptosis in Human T-Cell Leukemia Cell Lines but Not in Normal CD3 and CD34 Positive Cells

“…Galectins are the most widely studied animal lectins and are demonstrated to affect survival, signal transduction, and proliferation in many cancers particularly in colorectal cancers [9] , [10] . Achatinin, a lectin from hemolymph of snail, Achatina fulica is highly cytotoxic against MCF7, a human mammary carcinoma cell line [11] . Musca Domestica Larva Lectin (MLL) has been shown to inhibit cell proliferation and induce apoptosis of human hepatoma BEL-7402 [12] .…”

Microbial diversity of garden snail mucus

“…Different parts of the snail have different compositions of their biologically active substances. For example, several studies focused on shell (Chen et al, 2016;Latire et al, 2014), body (Iijima et al, 2003;Li et al, 2014), mucus (Dolashki et al, 2020;Greistorfer et al, 2017;Zhong et al, 2013;Ziomek et al, 2017), and hemolymph (Dharmu et al, 2007;Dolashka et al, 2011;Sperstad et al, 2011).…”