Cytotoxic Effect, Antioxidant Potential, and Phytochemical Study of the Ethyl Acetate Extract of Pleiogynium Timorense Seeds

Abstract

Objective: The aim of the current research was to evaluate the cytotoxicity of Pleiogynium timorense seeds against different human cancer cell lines, its antioxidant activity and to investigate its phytoconstituents.
 Methods: Ethyl acetate extract of Pleiogynium timorense seeds was assayed for the cytotoxic effect against liver cancer cell line (HepG2), ovarian cancer cell line (SKOV-3) and prostate cancer cell line (PC-3) using SRB (Sulforhodamine B) assay. The antioxidant activity was evaluated by the DPPH radical scavenging assay using Trolox as a standard. The phytochemical components of the plant extract were examined using various phytochemical screening methods. The polyphenolic contents of the extract were analyzed using high performance liquid chromatography (HPLC).
 Results: The result revealed that the seed extract exhibited very potent effect against HepG-2 cancer cell line with IC50=1.62 μg/mL, and against SKOV-3 cancer cell line with IC50 =6.37 μg/mL, while a moderate effect against PC-3 cancer cell line with IC50=46 μg/mL, by comparing with that of Doxorubicin. Moreover, IC50 values of Trolox and the seed extract were 24.42 ± 0.87 and 90.4±0.32 μg/ml, respectively. The results revealed the presence of the flavonoids, tannins and triterpenes and/or sterols in the seed extract. While, it revealed the absence of coumarins, alkaloids, saponins and carbohydrate and/or glycosides from the extract.
 Conclusion: In conclusion, the current study highlights the effect of ethyl acetate extract of Pleiogynium timorense seeds as antioxidant and a potent cytotoxic agent against different human cell lines aiming to be the first step towards the discovery of safe natural anticancer drug. 
 
 Peer Review History: 
 Received: 3 May 2022; Revised: 15 June; Accepted: 22 June, Available online: 15 July 2022
 Academic Editor: Dr. Tamer Elhabibi, Suez Canal University, Egypt, tamer_hassan@pharm.suez.edu.eg
 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. 
 Received file: Reviewer's Comments:
 Average Peer review marks at initial stage: 5.5/10
 Average Peer review marks at publication stage: 7.0/10
 Reviewers:
 Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my
 Prof. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, alialkaf21@gmail.com
 Similar Articles: 
 CYTOTOXIC EFFECT AND PHYTOCHEMICAL STUDY OF PETROLEUM ETHER EXTRACT OF TILIA CORDATA MILL 
 ANTIOXIDANT EFFICACY OF VITELLARIA PARADOXA NUTS DERIVATIVE PRODUCTS (BUTTER, HULLS AND PRESS CAKES) 
 ANTIHYPERGLYCEMIC AND ANTI-OXIDANT POTENTIAL OF ETHANOL EXTRACT OF VITEX THYRSIFLORA LEAVES ON DIABETIC RATS