Cytotoxic effect and antioxidant activity of Andean berry (Vaccinium meridionale Sw) wine

Abstract

Vaccinium meridonale Sw or Andean berry has antioxidant properties due to its high content of polyphenols, as anthocyanins and phenolic acids. Polyphenols have been associated with the prevention of chronic and cardiovascular diseases. In the last years, alcoholic drinks have been studied for their composition and health benefits. By this, the aim of this research was to obtain three types of alcoholic beverages from Andean berry, which have different treatments. The methods used to obtain the beverages were macerated fruit machine (MAC), preheating of the fruit (CAL) and by combining both of them (MIX). The antioxidant activity was evaluated by FRAP, DPPH, ORAC methods and anthocyanins and total phenols were measured. Finally, the antiproliferative effect was evaluated on a colon cancer cell line (SW480). Findings suggest that ethanol content of final products is not altered by treatment of unfermented Andean berry juice (must). The alcohol concentrations for MAC, CAL and MIX drinks were 90±1.7, 89±3.6 and 94±4.1 g/L, respectively. The results showed that CAL and MIX methods favor the extraction of secondary metabolites and consequently increase the antioxidant activity. The fermentation process affected the antioxidant power and total phenolic content in beverages CAL and MIX. However, no significant changes in these parameters were observed in the MAC drink. These beverages can eventually reduce the cancer cell viability between 15.1 (20 µg/L) and 37.2% (200 µg/mL). Thus, it was concluded that MIX treatment has higher antioxidant power and it could reduce the cancer cell viability.

Physico-chemical characterization and antioxidant capacity of the colombian berry (Vaccinium meridionale Swartz) with a high-polyphenol content: potential effects in people with metabolic syndrome

“…The antioxidant capacity of the freeze-dried agraz by the DRAC method was superior (63,717 ± 13.03 μmol TE/100 g freeze-dried) than the reported (41,775.2 ± 6,168.2 μmol TE/100 g lyophilized) by Maldonado-Celis et al (2014) for the same fruit, which could be attributed to the thermic treatment applied to the agraz fruits, as it has been suggested previously, the thermic treatments favor the extraction of polyphenols present in the agraz fruits with the subsequent increase in their antioxidant capacity (Vahos et al, 2016). Regarding the DRAC value found for the freeze-dried agraz (637.17 μmol TE/g), this value is in the range reported by Pertuzatti et al (2014) for 10 cultivars of the Brazilian V. corymbosum L. and V. ashei Reade (533-1,028 μmol TE/g of dry weight).…”

“…Additionally, for the intervention study, it was also necessary to design a placebo with similar physicochemical and sensorial characteristics of the agraz nectar, but free of polyphenolics. The nectar color (a*, b*, L, c*, h) and its rheological behavior (viscosity) determined the sensorial parameters perceived by the final consumer, such as the sensation in the mouth and visual appearance (Vahos et al, 2016). For this, it was used different food additives providing similar calories as the agraz nectar, but without polyphenol contribution.…”

PRODUCTION OF VINEGAR FROM AN ALCOHOLIC 1 BEVERAGE OF 2 ANDEAN BERRY (Vaccinium meridionale SW), MEASUREMENT OF 3 ANTIOXIDANT ACTIVITY AND EVALUATION OF CYTOTOXIC EFFECT ON 4 COLON CANCER CELLS SW480

“…ANOVA analysis indicates that the correlation of the factors (MAC, MIX, and CAL) was not statistically significant (p<0,05). Suggesting that the different methods of fruit processing do not affect the biotransformation capacity of alcohol to vinegar; in previous works, we found the same behavior for the alcoholic fermentation of berries of V. meridionale (17).…”

“…It was activated in the water at 37 °C for 10 min. Subsequently, alcoholic fermentation was performed for 20 days at room temperature (17). The Juice was prepared in the same way avoiding the fermentation process.…”

Evaluation of Agraz Consumption on Adipocytokines, Inflammation, and Oxidative Stress Markers in Women with Metabolic Syndrome

“…The berry Vaccinium meridionale Swartz is a native plant from the Andean region of South America, growing on mountain hillsides between 2200 and 3400 m above sea level [ 31 ]. In Colombia, this fruit is called “agraz” and it has generated significant interest in the food and pharmaceutical industries due to its antioxidant, anti-inflammatory, and anticarcinogenic potential [ 32 , 33 ]. Thus, it might have the ability to modulate MetS risk factors [ 34 ].…”