Cytotoxic cytochalasins from the endophytic fungus Eutypella scoparia PSU-H267

Kongprapan, Thippaya; Rukachaisirikul, Vatcharin; Saithong, Saowanit; Phongpaichit, Souwalak; Poonsuwan, Wimarak; Sakayaroj, Jariya

doi:10.1016/j.phytol.2015.06.010

Cited by 30 publications

(11 citation statements)

References 19 publications

(27 reference statements)

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“…On the other hand, four cytochalasins (2–5) and scoparasin C was identified from Eutypella scoparia PSU-H267 broth culture. Scoparasin C and cytochalasins 3 and 4 showed potential activity against Vero cell line 73 . Further cytochalasin alkaloids, Chaetoglobosins was previously isolated from Chaetomium globosum 74 .…”

Section: Discussionmentioning

confidence: 99%

Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling

Mohammed

Sonbol

Alwakeel

et al. 2021

Sci Rep

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Soil is considered an extensively explored ecological niche for microorganisms that produce useful biologically active natural products suitable for pharmaceutical applications. The current study aimed at investigating biological activities and metabolic profiles of three fungal strains identified from different desert sites in Saudi Arabia. Soil fungal isolates were collected from AlQasab, Tabuk, and Almuzahimiyah in Saudi Arabia and identified. Furthermore, their antibacterial activity was investigated against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumonia, and Escherichia coli in blood, nutrient, and Sabouraud dextrose agars. Moreover, fungal extracts were evaluated on cell viability/proliferation against human breast carcinoma and colorectal adenocarcinoma cells. To identify the biomolecules of the fungal extracts, High-performance liquid chromatography HPLC–DAD coupled to analytical LC–QTOF-MS method was employed for fungal ethyl acetate crude extract. Identified fungal isolates, Chaetomium sp. Bipolaris sp. and Fusarium venenatum showed varied inhibitory activity against tested microbes in relation to crude extract, microbial strain tested, and growth media. F. venenatum showed higher anticancer activity compared to Chaetomium sp. and Bipolaris sp. extracts against four of the tested cancer cell lines. Screening by HPLC and LC/MS-QTOF identified nine compounds from Chaetomium sp. and three from Bipolaris sp. however, for F. venenatum extracts compounds were not fully identified. In light of the present findings, some biological activities of fungal extracts were approved in vitro, suggesting that such extracts could be a useful starting point to find compounds that possess promising agents for medical applications. Further investigations to identify exact biomolecules from F. venenatum extracts are needed.

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Section: Discussionmentioning

confidence: 99%

Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling

Mohammed

Sonbol

Alwakeel

et al. 2021

Sci Rep

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“…Stierle was the first to isolate an endophytic fungus (Taxomyces andreanae) that produced Taxol from Taxus brevifolia (Stierle, Strobel & Stierle, 1993). Since then, an increasing number of researchers began to turn their attention to secondary metabolites of endophytic fungi, and more recent studies have certified that most essential secondary metabolites from endophytic fungi that have been reported are important and novel leading compounds, for instance, Taxol, camptothecin (Puri et al, 2005), vinblastine (Kumar et al, 2013), salidroside (Cui et al, 2016), piperine (Chithra et al, 2014), new halogenated dihydroisocoumarins (Zhao et al, 2018), cytotoxic cytochalasins (Kongprapan et al, 2015), podophyllotoxin (Puri et al, 2006) and so on. Recently, endophytic fungi were regarded as a rich source of bioactive metabolites that can be modified to produce desirable novel analogs for medicine, especially as potential anticancer agents (Fathallah et al, 2019).…”

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confidence: 99%

Isolation and identification of flavonoid-producing endophytic fungi from medicinal plant Conyza blinii H.Lév that exhibit higher antioxidant and antibacterial activities

Tang

Wang

Yang

et al. 2020

PeerJ

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Background. Conyza blinii H. Lév is a medicinal plant that has a variety of pharmacological activities, but its study is at a standstill due to the shortage of resources. Method. This study utilized the surface sterilization method to isolate endophytic fungi, and they were preliminarily identified by morphology. Flavonoid-producing strains were screened by NaNO 2 -Al(NO) 3 colorimetry and further identified by the ITS sequence. Additionally, we used five antioxidant assays (DPPH, Hydroxyl radical, ABTS, FRAP and T-AOC assays) to systematically evaluate the antioxidant capacity of total flavonoids , and we also determined their antibacterial activity. Results. In this study, 21 endophytic fungi were isolated from wild Conyza blinii H. Lév for the first time. There were six flavonoid-producing strains, especially CBL11, whose total flavonoid content reached 50.78 ± 2.4 mg/L. CBL12, CBL12-2 and CBL1-1 all exhibited excellent antioxidant activity. The effect of CBL12 was similar to that of ascorbic acid at low concentrations, and its radical scavenging rates for DPPH and ABTS were 94.56 ± 0.29 % and 99.88 ± 0.27%, respectively, while its IC 50 values were only 0.11 ± 0.01 mg/mL and 0.2 ± 0.01 mg/mL. Through LC-MS, we found that CBL12 could produce many high-value flavonoids, such as 3-methoxyflavone, nobiletin, formononetin, scopoletin, and daidzein. Additionally, CBL9 had good antibacterial activity against both gram-positive and gram-negative bacteria. Notably, we obtained the high-yield strains CBL12 and CBL9, which not only had high yields (10.64 ± 1.01 mg/L and 10.17 ± 0.11 mg/L, respectively) but also had excellent biological activity. Hence, the results of this study provide new ideas for endophytic fungi that can be exploited as a source of flavonoids and other medicinal components from Conyza blinii H. Lév. Moreover, this study can serve as a reference for the development of rare medicinal materials. G. 2020. Isolation and identification of flavonoid-producing endophytic fungi from medicinal plant Conyza blinii H.Lév that exhibit higher antioxidant and antibacterial activities. PeerJ 8:e8978 http://doi.Patil MP, Patil RH, Maheshwari VL. 2015. Biological activities and identification of bioactive metabolite from endophytic aspergillus flavus L7 isolated from aegle marmelos. Current Microbiology 71:39-48 GN. 2006. The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans.

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“…The biodiversity within the kingdom of Fungi is considerable; of the more than 1.5 million species, divided into the five phyla thought to exist, most have not ever been described . Endophytic fungi are a particularly promising source of structurally diverse and biologically active metabolites. − The endophytes of genus Eutypella produce a number of biologically active secondary metabolites, such as cytochalasins, γ-lactones, benzopyrans, and cytosporin-related derivatives, as well as terpenoids including ent -eudesmane sesquiterpenes and pimarane diterpenes. − …”

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confidence: 99%

Sesquiterpenes and Steroids from an Endophytic Eutypella scoparia

Zhang

Huo

et al. 2021

J. Nat. Prod.

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A chemical investigation on the EtOAc extract of the endophytic fungus Eutypella scoparia SCBG-8 led to the isolation of eight new sesquiterpenes eutyscoparins A–H (1–8), one C-28 steroid eutyscoparene A (9), one triterpenoid eutyscoparene B (10), six known terpenoids, and two known steroids. Their structures including absolute configurations were established on the basis of spectroscopic data analysis, single-crystal X-ray diffraction experiments, and electronic circular dichroism (ECD) calculations. Compound 7 displayed antibacterial activity against S. aureus and MRSA (methicillin-resistant Staphylococcus aureus) with MIC values of 6.3 μg/mL.

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Cytotoxic cytochalasins from the endophytic fungus Eutypella scoparia PSU-H267

Cited by 30 publications

References 19 publications

Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling

Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling

Isolation and identification of flavonoid-producing endophytic fungi from medicinal plant Conyza blinii H.Lév that exhibit higher antioxidant and antibacterial activities

Sesquiterpenes and Steroids from an Endophytic Eutypella scoparia

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