Cytotoxic constituents of Glycosmis ovoidea collected in Vietnam

Strategies for the discovery of potential anticancer agents from plants collected from Southeast Asian tropical rainforests as a case study

“…As part of the study performed, the structure of compound 10 was confirmed by X-ray crystallography. 58 In addition, a tenth constituent, 5,3′-dihydroxy-3,6,7,8,4′-pentamethoxyflavone ( 11 ), proved to be the only cytotoxic compound from G. ovoidea , as demonstrated by its evaluation against five human cancer cell lines. 58 In a preliminary biological investigation, the mode of action of compound 11 was examined in some detail in terms of its ability to inhibit the growth of MCF-7 human breast cancer cells.…”

“…Using a confirmatory western blot experiment, kimcuongin ( 10 ) was determined to potentiate the effects of compound 11 through both the NF-κB and PARP-1 signal transduction pathways. 58…”

“…58 In addition, a tenth constituent, 5,3 ′dihydroxy-3,6,7,8,4 ′ -pentamethoxyavone (11), proved to be the only cytotoxic compound from G. ovoidea, as demonstrated by its evaluation against ve human cancer cell lines. 58 In a preliminary biological investigation, the mode of action of compound 11 was examined in some detail in terms of its ability to inhibit the growth of MCF-7 human breast cancer cells. 59 Compounds 10 and 11 were puried from the same chromatographic fraction, and it was found experimentally that Natural Product Reports Review when they were co-treated as pure substances, this led to an approximately 100-fold potentiation of the MCF-7 cellular potency exhibited by compound 11 alone.…”

“…Using a conrmatory western blot experiment, kimcuongin (10) was determined to potentiate the effects of compound 11 through both the NF-kB and PARP-1 signal transduction pathways. 58 3 Sourcing of tropical plant samples [28][29][30][31] During both phases I and II of the anticancer project, the species obtained, constituting both primary screening plant samples and recollected plant samples in large quantities, were sourced through collaborative research agreements (memoranda of agreements; MOA). These were negotiated and consolidated between the University of Illinois at Chicago (UIC) and a collaborating host institution in the country from where the collections were made.…”

Application of Zebrafish as a Model for Anti-Cancer Activity Evaluation and Toxicity Testing of Natural Products

“…Similarly, Carcache et al, (2022) preliminarily evaluated the in vivo toxicity of kimcoungin ( 41 ) isolated from Glycosmis ovoidea Pierre via the zebrafish model [ 97 ]. They treated zebrafish embryos with 50 µM kimcoungin and observed whether the embryos were abnormal at 24 hpf, and found that kimcoungin was non-toxic to zebrafish embryos at 50 µM.…”