Cytotoxic, Antitumor and Toxicological Profile of Passiflora alata Leaf Extract

Amaral, Ricardo G; Gomes, Silvana Vieira Floresta; Andrade, Luciana Nalone; Santos, Sara Albuquerque dos; Severino, Patrícia; Júnior, Ricardo Luiz Cavalcanti de Albuquerque; Souto, Eliana B.; Brandão, Geraldo Célio; Santos, Sandra Lauton; David, Jorge M; Carvalho, Adriana Andrade

doi:10.3390/molecules25204814

Cited by 19 publications

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References 44 publications

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“…Anticancer activity of other plant latexes was reported previously, such as latex of Calotropis procera [ 46 ] and Himatanthus drasticus [ 47 ]. Passiflora alata leaf latex exhibited cytotoxic effect against PC-3 cell lines with IC 50 < 30 µg/mL [ 48 ]. The obtained antiproliferative effect of latex against PC-3 and MCF-7 was further documented and morphologically examined.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, and as additional proof for the biological screening examinations, we performed a docking process for chlorogenic acid with E.coli DNA (7C7N), and rutin with human prostate-specific antigen (3QUM) and breast cancer-associated protein (1JNX), which were selected based on past research and literature [ 36 , 48 ], in order to look into the binding mode and conformation structure that performs a vital function in the interaction among proteins and compounds ( Figure 9 ).…”

Section: Resultsmentioning

confidence: 99%

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Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities of Thevetia peruviana Latex with Molecular Docking of Antimicrobial and Anticancer Activities

et al. 2022

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Natural origin molecules represent reliable and excellent sources to overcome some medicinal problems. The study of anticancer, anticoagulant, and antimicrobial activities of Thevetia peruviana latex were the aim of the current research. An investigation using high-performance liquid chromatography (HPLC) revealed that the major content of the flavonoids are rutin (11.45 µg/mL), quersestin (7.15 µg/mL), naringin (5.25 µg/mL), and hisperdin (6.07 µg/mL), while phenolic had chlorogenic (12.39 µg/mL), syringenic (7.45 µg/mL), and ferulic (5.07 µg/mL) acids in latex of T. peruviana. Via 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging, the experiment demonstrated that latex had a potent antioxidant activity with the IC50 43.9 µg/mL for scavenging DPPH. Hemolysis inhibition was 58.5% at 1000 µg/mL of latex compared with 91.0% at 200 µg/mL of indomethacin as positive control. Negligible anticoagulant properties of latex were reported where the recorded time was 11.9 s of prothrombin time (PT) and 29.2 s of the activated partial thromboplastin time (APTT) at 25 µg/mL, compared with the same concentration of heparin (PT 94.6 s and APPT 117.7 s). The anticancer potential of latex was recorded against PC-3 (97.11% toxicity) and MCF-7 (96.23% toxicity) at 1000 μg/mL with IC50 48.26 μg/mL and 40.31 µg/mL, respectively. Disc diffusion assessment for antimicrobial activity recorded that the most sensitive tested microorganisms to latex were Bacillus subtilis followed by Escherichia coli, with an inhibition zone (IZ) of 31 mm with minimum inhibitory concentration (MIC) (10.2 μg/mL) and 30 mm (MIC, 12.51 μg/mL), respectively. Moreover, Candida albicans was sensitive (IZ, 28 mm) to latex, unlike black fungus (Mucor circinelloides). TEM examination exhibited ultrastructure changes in cell walls and cell membranes of Staphylococcus aureus and Pseudomonas aeruginosa treated with latex. Energy scores of the molecular docking of chlorogenic acid with E. coli DNA (7C7N), and Rutin with human prostate-specific antigen (3QUM) and breast cancer-associated protein (1JNX), result in excellent harmony with the experimental results. The outcome of research recommended that the latex is rich in constituents and considered a promising source that contributes to fighting cancer and pathogenic microorganisms.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities of Thevetia peruviana Latex with Molecular Docking of Antimicrobial and Anticancer Activities

et al. 2022

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“…JELENA ĐORĐEVIĆ 1,2 , STOIMIR KOLAREVIĆ 3 , JOVANA JOVANOVIĆ MARIĆ 3 , MARIANA OALĐE PAVLOVIĆ 4 , DUŠAN SLADIĆ 5 , IRENA NOVAKOVIĆ 6 * and BRANKA VUKOVIĆ-GAČIĆ…”

Section: S1 Supplementary Materials Tounclassified

“…Biologically active compounds, originating from a variety of natural sources: plants, animals, and microorganisms have great potential for use in the treatment of malignancies. 3 According to the work of Amaral et al, 4 from 1980 to 2019, a total of 174 new compounds with indications for cancer treatment were commercialized, with 53 % of these drugs being natural products, compounds based on them, or compounds that mimic their action. Since the biodiversity of marine organisms is approximately half of the total biodiversity on Earth, marine organisms are a good basis for the creation of modern chemotherapeutics and have been involved in various clinical trials.…”

Section: Introductionmentioning

confidence: 99%

“…This study was financially supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. , СТОИМИР КОЛАРЕВИЋ 3 , ЈОВАНА ЈОВАНОВИЋ МАРИЋ 3 , МАРИАНА ОАЛЂЕ ПАВЛОВИЋ4 , ДУШАН СЛАДИЋ 5 , ИРЕНА НОВАКОВИЋ 6* и БРАНКА ВУКОВИЋ-ГАЧИЋ 2…”

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Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone

Djordjević

Kolarević

Jovanovic-Maric

et al. 2022

JSCS

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Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 ?M). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 ?M). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.

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Isolation, Anticancer Evaluation, Molecular Docking, Drug likeness and ADMET Studies of Secondary Metabolites from Psoralea corylifolia seeds

et al. 2022

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Phytochemical investigation of Psoralea corylifolia seeds as a potential source of anticancer lead molecules led to the isolation of total fourteen secondary metabolites (1-14) bakuchiol (1), isopsoralen (2), psoralen (3), bavachinin (4), 4-Omethylbavachalcone (5), bavachalcone (6), 12, 13-Dihydro-12, 13-epoxybakuchiol (7), corylin (8), psoralidin ( 9) neocorylin (10), corylifol A (11), neobavaisoflavone (12), bavachin (13) and 12hydroxyisobakuchiol ( 14). Their structures were confirmed by 1DNMR, MASS spectra and comparison with the literature. Compounds 10, 11, 13 and 14 exhibited promising in vitro anticancer activity further supported by in-silico docking results against BRCA-1 (PDB ID: 4OFB). Bavachin 13 was found as the most promising cytotoxic metabolite with IC 50 value 2.22 μM against MCF-7 cells. In-vitro mechanism of action studies of 13 against MCF-7 cell line showed apoptotic cleavage. In addition, most of the compounds showed good ADMET and drug likeness profile. Cumulative in-vitro and in-silico results provided compound 13 as a potential anticancer agent against breast cancer cells.

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Cytotoxic, Antitumor and Toxicological Profile of Passiflora alata Leaf Extract

Cited by 19 publications

References 44 publications

Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities of Thevetia peruviana Latex with Molecular Docking of Antimicrobial and Anticancer Activities

Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities of Thevetia peruviana Latex with Molecular Docking of Antimicrobial and Anticancer Activities

Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone

Isolation, Anticancer Evaluation, Molecular Docking, Drug likeness and ADMET Studies of Secondary Metabolites from Psoralea corylifolia seeds

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