Cytotoxic, Anti-Inflammatory and Adipogenic Effects of Inophyllum D, Calanone, Isocordato-oblongic acid, and Morelloflavone on Cell Lines

Taher, Muhammad; Aminuddin, Amnani; Susanti, Deny; Aminudin, Nurul Iman; On, Shamsul; Ahmad, Farediah; Hamidon, Hanisuhana

doi:10.20307/nps.2016.22.2.122

Cited by 30 publications

(17 citation statements)

References 12 publications

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“…Viability of cells for the extracts (1, 2.5, 5, 10, 25, 50, 100, and 200 μg/mL), compounds 1 – 8 (1, 2.5, 5, 10, 25, 50, 100, and 200 μ m ), and l ‐NMMA (50, 100, 200, 400, and 800 μ m ) as a positive control was determined by an Ez‐Cytox cell viability detection kit purchased from Dail Lab Service Co . (Seoul, Korea), as reported previously …”

Section: Methodssupporting

confidence: 66%

“…Viability of cells for the extracts (1, 2.5, 5, 10, 25, 50, 100, and 200 lg/mL), compounds 1 -8 (1, 2.5, 5, 10, 25, 50, 100, and 200 lM), and L-NMMA (50, 100, 200, 400, and 800 lM) as a positive control was determined by an Ez-Cytox cell viability detection kit purchased from Dail Lab Service Co. (Seoul, Korea), as reported previously. [20] [21] Measurement of Nitric Oxide Production RAW264.7 cells were plated into a 96-well plate at a density of 3 9 10 4 cells/well and preincubated at 37°C for 24 h. After serum starvation for 12 h, cells were pretreated with the extracts (1, 2.5, 5, 10, 25, 50, 100, and 200 lg/mL), compounds 1 -8 (1, 2.5, 5, 10, 25, 50, 100, and 200 lM), and L-NMMA (50, 100, 200, 400, and 800 lM) as a positive control for 1 h, and then stimulated with 100 ng/mL of LPS for another 24 h. To evaluate NO levels in the culture media, nitrite, a soluble oxidation product of NO, was measured using the Griess reaction. [30] [31]…”

Section: Extraction and Isolationmentioning

confidence: 99%

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Bioactivity‐Guided Isolation of Anti‐Inflammatory Constituents of the Rare Mushroom Calvatia nipponica in LPS‐Stimulated RAW264.7 Macrophages

Lee

et al. 2018

Chemistry & Biodiversity

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Calvatia species, generally known as puffball mushrooms, are used both as sources of food and as traditional medicine. Among the Calvatia genus, Calvatia nipponica (Agaricaceae) is one of the rarest species. Using bioassay-guided fractionation based on anti-inflammatory effects, five alkaloids (1 - 5), two phenolics (6 and 7), and a fatty acid methyl ester (8) were isolated from the fruiting bodies of C. nipponica. Compound 8 was identified from C. nipponica for the first time, and all isolates (1 - 8) were tested for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Compound 7 showed mild inhibition while compound 8 significantly inhibited NO production with an IC value of 27.50 ± 0.08 μm. The mechanism of NO inhibition of compound 7 was simulated by molecular docking analysis against nitric oxide synthase (iNOS), which revealed the interactions of 7 with the key amino acid residue and the heme in the active site. With the most potent inhibition against LPS-induced inflammation, compound 8 was further investigated with respect to its mechanism of action, and the activity was found to be mediated through the inhibition of iNOS and COX-2 expression.

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Section: Methodssupporting

confidence: 66%

Section: Extraction and Isolationmentioning

confidence: 99%

Bioactivity‐Guided Isolation of Anti‐Inflammatory Constituents of the Rare Mushroom Calvatia nipponica in LPS‐Stimulated RAW264.7 Macrophages

Lee

et al. 2018

Chemistry & Biodiversity

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“…The EtOH extract was then solvent-partitioned for bioassay-guided fractionation with hexane, dichloromethane (CH 2 Cl 2 ), ethyl acetate (EtOAc), and n -butanol, yielding soluble fractions of hexane (HX), CH 2 Cl 2 (DCM), EtOAc (EA), and n -butanol (BuOH), respectively. Prior to investigating the anti-inflammatory effects of the four solvent-partitioned fractions, we examined the cellular toxicity of these fractions in RAW264.7 cells with MTT assay [ 23 , 24 , 25 , 26 ]. No significant cell death was observed for the HX, DCM, and BuOH fractions up to concentrations of 50 μg/mL, while the EA fraction caused cell death at a concentration of 50 μg/mL (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells

Lee

Jang

et al. 2017

Molecules

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The edible fungus Phellinus baumii Pilat (Hymenochaetaceae) has been used in Korean traditional medicines for strengthening health and prolonging life. An extract of the fruiting bodies of P. baumii was subjected to bioassay-guided fractionation based on its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The resulting fractions were chemically investigated, leading to isolation of three phenolic compounds (1–3), a sesquiterpene (4), two steroids (5–6), a fatty acid (7), and a cerebroside (8). Spectroscopic analyses including 1D and 2D NMR spectroscopy and LC/MS were used to determine their chemical structures. Compounds 2, 4, 5, 7 and 8 were identified in P. baumii for the first time. Since all compounds were isolated from active fractions with anti-inflammatory activity, their ability to inhibit LPS-stimulated nitric oxide (NO) production in RAW264.7 cells were evaluated in vitro. Compounds 1, 2, 3, 5 and 7 inhibited LPS-stimulated NO production, and compounds 1–3 had IC50 values <10 μM. Treatment of LPS-stimulated RAW264.7 cells with compounds 1–3 inhibited phosphorylation of IKKα and IκBα. In addition, treatment of compounds 1–3 reduced LPS-induced increases of nuclear factor-kappa B (NF-κB) p65, iNOS and COX-2 protein expressions. Collectively, compounds 1–3 inhibited NF-κB-dependent inflammation in RAW264.7 cells. Thus, P. baumii is a potential source of natural anti-inflammatory agents, and active compounds 1–3 could be promising lead compounds for the development of novel anti-inflammatory agents.

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“…Compounds 1 , 2 , 3 and 7 at 100 μM had little or no toxicity, whereas compound 4 , 5 , and 6 inhibited the growth of cells at higher concentrations, the viability rate of all chemical constitutes did not exceed 65.0% at doses up to 100 μM, respectively ( Figure 8 ). Taher et al (2016) reported [ 27 ] that the biflavonoid, morelloflavone, isolated from Garcinia prainiana showed no obvious inhibition of RAW 264.7 macrophage cell viability [ 27 ]. Also, the cytotoxicity of the novel synthesized flavone derivatives was investigated in terms of the structure–anti-inflammatory activity relationship.…”

Section: Resultsmentioning

confidence: 99%

Characterization of New Polyphenolic Glycosidic Constituents and Evaluation of Cytotoxicity on a Macrophage Cell Line and Allelopathic Activities of Oryza sativa

Chung

Kwon

et al. 2018

Molecules

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Four new constituents, as 5, 7-dihydroxy-4′-methoxyflavonol-3-O-β-d-arabinopyranosyl-(2′′→1′′′)-O-β-d-arabinopyrnosyl-2′′′-O-3′′′′, 7′′′′-dimethylnonan-1′′′′-oate (1), 5-hydroxy-7, 4′-dimethoxyflavone-5-O-α-d-arabinopyranosyl-(2"→1′′′)-O-α-d-arabinopyranosyl-2′′′-O-3′′′′, 7′′′′-dimethylnonan-1′′′′-oate (2), 5-hydroxy-7, 4′-dimethoxyflavone-5-O-β-d-arabinofuranosyl-(2"→1′′′)-O-β-d-arabinopyranosyl-2′′′-O-lanost-5-ene (3) and 4′,4′′-diferuloxy feruloyl-O-α-d-arabinopyranosyl-(2a→1b)-O-α-d-arabinopyranosyl-(2b→1c)-O-α-d-arabinopyranosyl-(2c→1d)-O-α-d-arabinopyranosyl-(2d→1e)-O-α-d-arabinopyranosyl-2e-3′′′, 7′′′-dimethylnonan-1′′′-oate (4), along with three known compounds (5–7) were isolated from Oryza sativa leaves and straw. The structures of new and known compounds were elucidated by 1D (1H and 13C NMR) and 2D NMR spectral methods, viz: COSY, HMBC, and HSQC aided by mass techniques and IR spectroscopy. The cytotoxicity of these constituents was assessed by using (RAW 264.7) mouse macrophage cell line, and allelopathic effects of compounds (1–7) on the germination and seedling growth characteristics such as seedling length and root length of barnyardgrass (Echinochloa oryzicola) were evaluated. Significant inhibitory activity was exhibited by compounds comprising flavone derivatives such as (1–3) on all of seed germination characteristics. The allelopathic effect of flavone derivatives were more pronounced on seedling length and root length than the germination characteristics. The higher concentration of flavone derivatives showed stronger inhibitory effects, whereas the lower concentrations showed stimulatory effects in some cases.

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Cytotoxic, Anti-Inflammatory and Adipogenic Effects of Inophyllum D, Calanone, Isocordato-oblongic acid, and Morelloflavone on Cell Lines

Cited by 30 publications

References 12 publications

Bioactivity‐Guided Isolation of Anti‐Inflammatory Constituents of the Rare Mushroom Calvatia nipponica in LPS‐Stimulated RAW264.7 Macrophages

Bioactivity‐Guided Isolation of Anti‐Inflammatory Constituents of the Rare Mushroom Calvatia nipponica in LPS‐Stimulated RAW264.7 Macrophages

Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells

Characterization of New Polyphenolic Glycosidic Constituents and Evaluation of Cytotoxicity on a Macrophage Cell Line and Allelopathic Activities of Oryza sativa

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