Cytotoxic and genotoxic effects of <i>Kalanchoe pinnata</i> (Lam.) Pers. fresh leaf juice in the cultured human blood lymphocytes

Bhavsar, Shruti J.; Chandel, Divya

doi:10.1080/01480545.2019.1696814

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…Thus, the results of this study reveal that the analyzed doses of K. pinnata fresh leaf juice are having potential antioxidant activity which is observed to increase at higher dose, but should be used at standardized doses and durations as it can show genotoxic potential at higher doses as per our previous study [7].…”

Section: Discussionsupporting

confidence: 57%

“…The extract (fresh leaf juice) was prepared as reported earlier [6] and the two doses: 50 µl (low dose -LD) and 70 µl (high dose -HD)standardized as final concentrations in our previous study [7] were used in this study. In the present study, all the experiments were performed in triplicate.…”

Section: Authentication and Extract Preparationmentioning

confidence: 99%

“…Fresh leaf juice showed maximum content of primary and secondary phytoconstituents and was selected as the best extract amongst four [6]. Furthermore, in our further study, we used to evaluate the cytotoxicity and genotoxicity of fresh leaf juice on human peripheral blood lymphocyte cultures for safety analysis and with that, standardized two doses as safe dose [7]. Therefore, to contribute further to the knowledge of Indian traditional plants, our present study is focused on K. pinnata fresh leaf juice to determine its antioxidant and free radical scavenging properties for its standardized safe doses.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity Evaluation of Kalanchoe Pinnata (Lam.) Pers. Fresh Leaf Juice

Bhavsar

Chandel²

2022

Asian J Pharm Clin Res

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Objective: The objective of this study was to determine the antioxidant and free radical scavenging properties of Kalanchoe pinnata (Lam.) Pers. fresh leaf juice for standardized doses using different types of assays. Methods: The investigation of the antioxidant activity was carried out for the previously standardized doses (by cytotoxicity and genotoxicity analysis) – 50 μl (low dose - LD) and 70 μl (high dose-HD) by Phosphomolybdenum (PM), Ferric Reducing Antioxidant Power (FRAP), 2, 2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging activity, and 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays. Results: The activity measured by PM assay for LD and HD of the extract was equivalent to 109 μg/ml and 141 μg/ml of ascorbic acid, respectively, while by FRAP assay, it was equivalent to 17.5 μg/ml and 47 μg/ml of ascorbic acid, respectively. The activity measured by DPPH assay was found equivalent to 83 μg/ml and 177.5 μg/ml of ascorbic acid for LD and HD, with the percent scavenging activity 4.55% and 17.04%, respectively. Similarly, the activity of LD and HD by ABTS assay was found equivalent to 277.5 μg/ml and 308 μg/ml of ascorbic acid with the percent scavenging activity 39.01% and 47.93%, respectively. Conclusion: The changes observed in the absorbance values for all the assays reflected the increasing antioxidant activity of the plant extract in dose-dependent manner, which justifies the traditional use of this herb in prevention of diseases induced by oxidative stress. The antioxidant activity of K. pinnata fresh leaf juice has been conducted for the 1st time and has not been previously reported.

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Section: Discussionsupporting

confidence: 57%

Section: Authentication and Extract Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity Evaluation of Kalanchoe Pinnata (Lam.) Pers. Fresh Leaf Juice

Bhavsar

Chandel²

2022

Asian J Pharm Clin Res

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“…Investigations of the in vitro cytotoxicological and genotoxicological effects of K. pinnata were performed using its AE [ 85 ], leaf juice [ 64 ], and EE [ 165 ]. All the results indicated significantly lower results than those found for a positive control, suggesting a weak genotoxic response or a non-genotoxic effect.…”

Section: Resultsmentioning

confidence: 99%

A Review of the Popular Uses, Anatomical, Chemical, and Biological Aspects of Kalanchoe (Crassulaceae): A Genus of Plants Known as “Miracle Leaf”

et al. 2023

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Species of the genus Kalanchoe have a long history of therapeutic use in ethnomedicine linked to their remarkable healing properties. Several species have chemical and anatomical similarities, often leading to confusion when they are used in folk medicine. This review aims to provide an overview and discussion of the reported traditional uses, botanical aspects, chemical constituents, and pharmacological potential of the Kalanchoe species. Published scientific materials were collected from the PubMed and SciFinder databases without restriction regarding the year of publication through April 2023. Ethnopharmacological knowledge suggests that these species have been used to treat infections, inflammation, injuries, and other disorders. Typically, all parts of the plant are used for medicinal purposes either as crude extract or juice. Botanical evaluation can clarify species differentiation and can enable correct identification and validation of the scientific data. Flavonoids are the most common classes of secondary metabolites identified from Kalanchoe species and can be correlated with some biological studies (antioxidant, anti-inflammatory, and antimicrobial potential). This review summarizes several topics related to the Kalanchoe genus, supporting future studies regarding other unexplored research areas. The need to conduct further studies to confirm the popular uses and biological activities of bioactive compounds is also highlighted.

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“…The cytokinesis blocked proliferative index (CBPI) was calculated using the following formula:

where BI is the number of binucleated cells and MONO is the number of mononucleated cells ( Cardoso Trento et al., 2019 ). The cytostasis index (CI%) is the percentage of cell replication inhibition and was calculated using the following formula ( Bhavsar and Chandel, 2019 ).

…”

Section: Methodsmentioning

confidence: 99%

Behavioral avoidance and biological safety of vetiver oil and its constituents against Aedes aegypti (L.), Aedes albopictus (Skuse) and Culex quinquefasciatus Say

Nararak

Giorgio²,

Thanispong³

et al. 2022

Current Research in Insect Science

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Cytotoxic and genotoxic effects of Kalanchoe pinnata (Lam.) Pers. fresh leaf juice in the cultured human blood lymphocytes

Cited by 5 publications

References 27 publications

Antioxidant Activity Evaluation of Kalanchoe Pinnata (Lam.) Pers. Fresh Leaf Juice

Antioxidant Activity Evaluation of Kalanchoe Pinnata (Lam.) Pers. Fresh Leaf Juice

A Review of the Popular Uses, Anatomical, Chemical, and Biological Aspects of Kalanchoe (Crassulaceae): A Genus of Plants Known as “Miracle Leaf”

Behavioral avoidance and biological safety of vetiver oil and its constituents against Aedes aegypti (L.), Aedes albopictus (Skuse) and Culex quinquefasciatus Say

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