Cytotoxic and genotoxic effects of [Ru(phi)3]2+ evaluated by Ames/Salmonella and MTT methods

Benkli, Kadriye; Tunalı, Yağmur; Cantürk, Zerrin; Artagan, Öge; Alanyalı, Filiz Susuz

doi:10.1016/j.ejmech.2008.09.017

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…Tobacco wastewater was also shown to be toxic to coliforms (Sponza, 2002). Therefore, this test has a large anticipating value for cancer formation (Aydogan and Kutlu 2007;Benkli et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Induction of Micronuclei, Base-pair Substitution Mutation and Excision-repair Deficient by Polluted Water from Asa River in Nigeria

Abass

Oladipo

Mutolib

et al. 2019

Annals of Science and Technology

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Asa river is a major river designated to supply millions of people of Ilorin, Kwara State, Nigeria potable water for drinking but its managements is of grave concern due to anthropogenic activities. Thus, evaluation of genotoxicity of this river was carried out by subjecting the water samples and fish therein to three bioassays (Micronucleus (MN) assay, Ames test and SOS-chromo test). Physicochemical parameters and heavy metals were analysed at three different stations (Aliara (SI), Unity (SII) and Tuyil (SIII)) of the river. In SII, most of the heavy metals analysed were above the acceptable limits compare to SI and SIII. The peripheral erythrocyte of the fishes (Oreochromis niloticus, Synodontis batensoda, Synodontis eupterus, Clarias gariepinus and Clarias angullaris) at SI and SII stations showed a significant (p<0.05) induction of MN and different nuclear abnormalities (NA). Water samples from the three stations subjected to Ames test (Salmonella typhimurium TA100) and SOS chromotests (Escherichia coli PQ37) at 25%, 50% and 100% concentrations showed statistically significant (p<0.05) induction of DNA damage at all concentrations in the two tester strains, thus indicating base-pair substitution mutation and excision-repairdeficient, respectively, by the water samples. Therefore, drinking of this water and/or consumption of fish from this river should be taken with caution to avoid a carcinogenic risk.

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Section: Discussionmentioning

confidence: 99%

Induction of Micronuclei, Base-pair Substitution Mutation and Excision-repair Deficient by Polluted Water from Asa River in Nigeria

Abass

Oladipo

Mutolib

et al. 2019

Annals of Science and Technology

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“…We studied the cytotoxic effects of the eight novel Ir(III) complexes and the free ancillary ligands against NCI-H460 (nonsmall lung), T-24 (bladder), HeLa (cervical), and HL-7702 (hepatocyte) using the MTT assay. − The results are shown in Table S1. We found that the IC 50 values of the free ancillary ligands against cancer cells were >50 μM except in HeLa cells.…”

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confidence: 99%

“…Furthermore, among the eight novel Ir(III) complexes, MPytMP-Ir exhibited significantly superior and sensitive cytotoxic activity with a much lower IC 50 (5.05 ± 0.22 nM) against NCI-H460 cells. Of course, the methyl electron-donating group does enhance the cytotoxic activity via a combination of steric and electronic effects, such as polypyridyl, pyrazine, and pyrazole ring. ,,,, Owing to its highly efficient and selective cytotoxic activity against the NCI-H460 cancer cells compared to cisplatin, we performed further in-depth studies using MPytMP-Ir . , We also compared its effectiveness to another synthesized compound MPydMP-Ir and used the sensitive NCI-H460 cell line to further study the potential interactive mechanisms of its biological cytotoxic influences. − …”

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confidence: 99%

Novel Quinoline-based Ir(III) Complexes Exhibit High Antitumor Activity in Vitro and in Vivo

Yang

Bin

Qin

et al. 2019

ACS Med. Chem. Lett.

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Eight novel Ir(III) complexes listed as [Ir(H–P)2(P)]PF6 (PyP-Ir), [Ir(H–P)2(dMP)]PF6 (PydMP-Ir), [Ir(H–P)2(MP)]PF6 (PyMP-Ir), [Ir(H–P)2(tMP)]PF6 (PytMP-Ir), [Ir(MPy)2(P)]PF6 (MPyP-Ir), [Ir(MPy)2(dMP)]PF6 (MPydMP-Ir), [Ir(MPy)2(MP)]PF6 (MPyMP-Ir), [Ir(MPy)2((tMP)]PF6 (MPytMP-Ir) with 2-phenylpyri-dine (H–P) and 3-methyl-2-phenylpyridine (MPy) as ancillary ligands and pyrido-[3,2-a]-pyrido[1′,2′:1,2]imidazo[4,5-c]phenazine (P), 12,13-dimethyl pyrido-[3,2-a]-pyrido[1′,2′:1,2]-imidazo-[4,5-c]-phenazine (dMP), 2-methylpyrido [3,2-a]-pyrido-[1′,2′:1,2]-imidazo-[4,5-c]-phenazine (MP), and 2,12,13-trimethylpyrido-[3,2-a]-pyrido-[1′,2′:1,2]-imidazo-[4,5-c]-phenazine (tMP) as main ligands, respectively, were designed and synthesized to fully characterize and explore the effect of their toxicity on cancer cells. Cytotoxic mechanism studies demonstrated that the eight Ir(III) complexes exhibited highly potent antitumor activity selectively against cancer cell lines NCI-H460, T-24, and HeLa, and no activity against HL-7702, a noncancerous cell line. Among the eight Ir(III) complexes, MPytMP-Ir exhibited the highest cytotoxicity with an IC50 = 5.05 ± 0.22 nM against NCI-H460 cells. The antitumor activity of MPytMP-Ir in vitro could be contributed to the steric or electronic effect of the methyl groups, which induced telomerase inhibition and damaged mitochondria in NCI-H460 cells. More importantly, MPytMP-Ir displayed a superior inhibitory effect on NCI-H460 xenograft in vivo than cisplatin. Our work demonstrates that MPytMP-Ir could potentially be developed as a novel potent Ir-based antitumor drug.

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Development of four ruthenium polypyridyl complexes as antitumor agents: Design, biological evaluation and mechanism investigation

Jiang

Zhang

et al. 2020

Journal of Inorganic Biochemistry

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Cytotoxic and genotoxic effects of [Ru(phi)3]2+ evaluated by Ames/Salmonella and MTT methods

Cited by 9 publications

References 30 publications

Induction of Micronuclei, Base-pair Substitution Mutation and Excision-repair Deficient by Polluted Water from Asa River in Nigeria

Induction of Micronuclei, Base-pair Substitution Mutation and Excision-repair Deficient by Polluted Water from Asa River in Nigeria

Novel Quinoline-based Ir(III) Complexes Exhibit High Antitumor Activity in Vitro and in Vivo

Development of four ruthenium polypyridyl complexes as antitumor agents: Design, biological evaluation and mechanism investigation

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