CYTOTOXIC AND GENOTOXIC ACTIVITY OF PHENOLIC FRACTIONS FROM Ulomoides dermestoides FAIRMAIRE, 1893 (COLEOPTERA, TENEBRIONIDAE), IN HACAT CELLS

Meza, Dary Luz Mendoza; España-Puccini, Pierine

doi:10.1016/j.recqb.2016.06.001

Cited by 11 publications

(9 citation statements)

References 41 publications

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“…Ulomoides dermestoides Chevrolat, a beetle also known as the “peanut weevil”, is used in the traditional medicine of Argentina, Brazil, China, Colombia, Japan, and Mexico to treat backache, cough, asthma, and diabetes, among others ( 5 , 6 ). The main compounds of the cuticle (alkenes and terpenes) and the defensive secretion (benzoquinones) of this beetle have been characterized in previous chemical studies ( 7 ), and its adverse anti-inflammatory, cytotoxic, and genotoxic activities have been described ( 6 – 9 ).…”

Section: Introductionmentioning

confidence: 99%

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression

Jasso-Villagomez

García-Lorenzana

Almanza-Perez

et al. 2018

Braz J Med Biol Res

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Ulomoides dermestoides is a beetle traditionally consumed to treat diabetes. In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to evaluate morphological changes. The most active fraction, the fat fraction, was analyzed by gas chromatography-mass spectrometry (GC-MS), and PPARγ and GLUT4 mRNA expressions were determined in 3T3-L1 adipocytes. The protein and fat fractions exhibited hypoglycemic effects in the acute as well as in the 30-day study. Only the fat fraction led to elevated insulin levels and reduced glycemia, as well as lower intake of water and food. In the liver, we observed recovery of close hepatic cords in the central lobule vein following treatment with the fat fraction, while in the pancreas there was an increased density and percentage of islets and number of cells per islet, suggesting cellular regeneration. The GC-MS analysis of fat revealed three fatty acids as the major components. Finally, increased expression of PPARγ and GLUT4 was observed in 3T3-L1 adipocytes, indicating an antidiabetic effect.

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Section: Introductionmentioning

confidence: 99%

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression

Jasso-Villagomez

García-Lorenzana

Almanza-Perez

et al. 2018

Braz J Med Biol Res

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“…In Central and South American traditional medicine, the darkling beetle Ulomoides dermestoides is used to treat inflammatory diseases and cancer. The literature has also reported on the anti-inflammatory and immunomodulatory activities of an aqueous extract of this beetle, suggesting the presence of components that are pharmacologically promising [2][3][4][5][6][7][8]. The insect is able to reproduce rapidly in artificial conditions similar to those described for the related species Alphitobius diaperinus [9].…”

Section: Introductionmentioning

confidence: 99%

Novel Extract from Beetle Ulomoides dermestoides: A Study of Composition and Antioxidant Activity

et al. 2021

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A biologically active extract from the darkling beetle Ulomoides dermestoides was obtained using the electro-pulse plasma dynamic extraction method. The beetle water extract contained a complex of antioxidant substances such as antioxidant enzymes and nonprotein antioxidants, as well as a complex of heat shock antistress proteins. This determines the rather high antioxidant activity of the aqueous extract of the beetle, i.e., 1 mg of dry matter/mL of the extract has an equivalent antioxidant activity to 0.2 mM Trolox (a water-soluble analog of vitamin E). It was shown that the beetle extract can lead to a 25–30% increase in the average lifespan of nematode Caenorhabditiselegans, under normal conditions, and a 12–17% increase under conditions of oxidative stress (with paraquat), and significantly inhibits the fructosylation reaction of serum albumin. Therefore, the beetle aqueous extract shows promise as a biologically active complex exhibiting antioxidant activity.

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“…For example, Apis mellifera bees, the larvae and adults of the large our beetle Tenebrio molitor, carrion ies of the Calliphoridae family, Galleria mellonella wax moths and Bombyx mori silkworm contain new antimicrobial peptides, chitin-melanin complexes, avonoids, aminoacids and organic acids [1][2][3][4][5]. Darkling beetles of the Tenebrionidae family, which are used in traditional medicine to treat a wide range of diseases, are of special interest [1][2][3][4][6][7][8]. These insects synthesize protective secretions, which are a mixture of repellent and blocking chemoreceptor substances.…”

Section: Introductionmentioning

confidence: 99%

“…They are found in cuticular inclusions or abdominal glands and are released when the beetles are stressed. It is believed that the secretions of these beetles are also necessary for the insect to prevent drying out and protect against pathogenic microorganisms [1][2][3][4][6][7][8]. It has been shown that these secrets are a source of pharmacologically active compounds that are promising for the treatment of respiratory diseases [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of the Tenebrionidae Beetle Antioxidant Complex in a Murine Neurotoxic Model of Parkinson's Disease

Kovalzon¹,

Aleks²,

Ambaryan³

et al. 2021

Preprint

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We have previously shown that the aqueous extract of the Ulomoides dermestoides darking beetle (the Tenebrionidae family) biomass contains a powerful complex of antioxidant substances of protein and non-protein nature. Considering the crucial role of ROS in the development of neurodegeneration, we set out to test the biological activity of this extract in a mouse neurotoxic model of Parkinson's disease. The beetle extracts were administrated continuously with food and their effects on parkinsonism caused by twice injected defoliant paraquat to experimental mice was evaluated. The motor activity of the animals was analyzed in behavioral tests using a rotarod and a vertical pole. The number of tyrosine hydroxylase-immunopositive neurons in the ventral part of the substantia nigra of the midbrains of experimental and control mice was studied by immunohistochemistry. In the model in vitro system with SH-SY5Y human neuroblastoma, the effect of the extracts on cell proliferation was examined in the absence and presence of the neurotoxin MPP+. The isolation of biologically active substances from raw biomass using cavitation effects made it possible to obtain extracts with protective properties in the model of an early stage of Parkinson's disease used in this study.

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CYTOTOXIC AND GENOTOXIC ACTIVITY OF PHENOLIC FRACTIONS FROM Ulomoides dermestoides FAIRMAIRE, 1893 (COLEOPTERA, TENEBRIONIDAE), IN HACAT CELLS

Cited by 11 publications

References 41 publications

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression

Novel Extract from Beetle Ulomoides dermestoides: A Study of Composition and Antioxidant Activity

Biological Activity of the Tenebrionidae Beetle Antioxidant Complex in a Murine Neurotoxic Model of Parkinson's Disease

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