Cytotoxic and chemotactic potencies of several aldehydic components of oxidised low density lipoprotein for human monocyte‐macrophages

Müller, Karin H.; Hardwick, Simon J.; Marchant, Christine E.; Law, Nadine S.; Waeg, Georg; Esterbauer, Hermann; Carpenter, Keri L. H.; Mitchinson, M. J.

doi:10.1016/0014-5793(96)00559-5

Cited by 56 publications

(33 citation statements)

References 30 publications

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“…We found that the five unsaturated aldehydes in oxidized oils are highly cytotoxic, in agreement with previous findings 12) . Based on their chemical structures, these aldehydes may show similar extents of toxicity.…”

Section: Discussionsupporting

confidence: 93%

Re-evaluation of Peroxide Value as an Indicator of the Quality of Edible Oils

Shiozawa¹,

Tanaka²,

Ohno³

et al. 2007

J. Food Hyg. Soc. Jpn

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The oxidation of oils has important e#ects on the quality of oily foods, such as instant noodles. In particular, the generation of aldehydes, which accompanies the oxidation of oils, is one of the first factors to reduce food quality. We examined various indicators of oil quality during temperature-accelerated storage and found that peroxide value (POV) was highly correlated with the total concentration of major odorants. Moreover, the correlation of POV with the total concentration of five unsaturated aldehydes (t-2-heptenal, t-2-octenal, t-2-decenal, t-2-undecenal and t,t-2,4-decadienal) that show strong cytotoxicity was greater than the correlation of POV with the total concentration of major odorants. The maximum allowable concentration of the five aldehydes was calculated based on the 'no observed adverse-e#ect level' of the aldehyde that showed the highest cytotoxicity, t,t-2,4-decadienal, along with the human daily oil intake. We showed that it is useful to utilize POV as an indicator to control food quality and safety.

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Section: Discussionsupporting

confidence: 93%

Re-evaluation of Peroxide Value as an Indicator of the Quality of Edible Oils

Shiozawa¹,

Tanaka²,

Ohno³

et al. 2007

J. Food Hyg. Soc. Jpn

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“…Steady-state concentrations range from 0.2 to 2.8 µM [27]. Although the concentration of HNE in the oxLDL was not measured in this study, Muller et al [22] reported HNE concentrations of 22-114 nmol/mg LDL protein and HHE concentrations of 4-49 nmol/mg LDL protein in copperoxidized LDL. The concentration of each aldehyde increased as the extent of oxidation increased.…”

Section: Discussionmentioning

confidence: 80%

“…Numerous compounds found in oxLDL, including lipid hydroperoxides and several aldehydes, have been shown to cause apoptosis [22,23]. In a pilot study [24], we recently showed that oxLDL enriched in EPA and DHA induced less apoptosis in U937 cells, a human promonocytic cell line, than oxLDL enriched with linoleic acid.…”

Section: Articlementioning

confidence: 97%

“…They suggested [53] that the role of the aldehydes during pathophysiological conditions could be due to selective alteration in cell signaling, protein or DNA damage, cell proliferation and cell differentiation, and induction of apoptosis. Muller et al [22] investigated the cytotoxic and chemotactic potencies of several aldehydes, including HNE and HHE. They found that HNE was more toxic than HHE.…”

Section: Discussionmentioning

confidence: 99%

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Reduced effect on apoptosis of 4-hydroxyhexenal and oxidized LDL enriched with n-3 fatty acids from postmenopausal women

Lee

Wander

2005

The Journal of Nutritional Biochemistry

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Background: Oxidized low-density lipoprotein (oxLDL) promotes apoptosis in atherosclerotic plaques in the vascular wall, a process mediated through its oxidized lipids. 4-Hydroxynonenal (HNE) and 4-hydroxyhexenal (HHE), derived from oxidation of n-6 and n-3 fatty acids, respectively, are among the major oxidized products in oxLDL.Hypothesis: This study hypothesized that eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA)-rich versus linoleic acid-rich oxLDL obtained from postmenopausal women and HNE versus HHE differentially influence apoptosis in U937 cells.Experimental Design: Thirty healthy postmenopausal women were supplemented with 14 g/day safflower oil (SO), 7 g/day of both fish oil and SO (low dose LFO) or 14 g/day fish oil (high dose HFO) for 5 weeks. Low-density lipoprotein, obtained after supplementation, was oxidized with 5 µM CuSO4 at 37°C for 6 h. The concentration of cholesteryl ester hydroperoxides (CEOOH) and conjugated dienes was measured in the oxidized LDL (oxLDL). U937 cells were incubated with the oxLDL, 10 µM of HHE, 7 µM of HHE plus 3 µM of HNE, 5 µM of both HHE and HNE or 10 µM of HNE and the extent of apoptosis measured three ways. Results:The concentration of CEOOH and conjugated dienes in oxLDL did not differ among the three treatment groups. The percent of apoptotic cells was approximately 40% lower when incubated with oxLDL obtained from the HFO-supplemented group than the SO-supplemented group measured by both the Annexin V and the DNA fragmentation assays (P=.04 and .004, respectively). Apoptosis of U937 cells was significantly lower in cells incubated with 10 µM of HHE, and mixtures of HHE and HNE than the 10 µM HNE when measured by the Annexin V, DNA fragmentation and 4,6-diamidino-2-phenylindole (DAPI) staining. Conclusions:These data suggest that the cardioprotective properties of n-3 fatty acids may derive in part from their less reactive oxidized lipid metabolites.

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“…These aldehydes are lipid peroxidation end products which display both a cytotoxic and a chemotactic power. They are likely to be produced by the oxidation of the LDL which have reached the intima of arteries and can contribute to the recruitment of monocyte-macrophages [30]; a direct cytotoxic effect on the foam cells of the atheroma was considered unlikely by Muller because it required higher levels of the aldehydes.…”

Section: Action Of Lipid Peroxidation On Atherosclerosismentioning

confidence: 99%

Tissue Occurrence of Carbonyl Products of Lipid Peroxidation and Their Role in Inflammatory Disease

Rossi¹

2012

Lipid Peroxidation

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Cytotoxic and chemotactic potencies of several aldehydic components of oxidised low density lipoprotein for human monocyte‐macrophages

Cited by 56 publications

References 30 publications

Re-evaluation of Peroxide Value as an Indicator of the Quality of Edible Oils

Re-evaluation of Peroxide Value as an Indicator of the Quality of Edible Oils

Reduced effect on apoptosis of 4-hydroxyhexenal and oxidized LDL enriched with n-3 fatty acids from postmenopausal women

Tissue Occurrence of Carbonyl Products of Lipid Peroxidation and Their Role in Inflammatory Disease

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