Cytotoxic and Apoptotic Effects of Carmofur and  Vitamin C Combination on HCT116 Colon Cancer Cells

Kalındemirtaş, Ferdane Danışman

doi:10.26650/experimed.2021.1038579

Cited by 2 publications

(2 citation statements)

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“…Finally, cell viability was decreased depending on the dosage and exposure time of indigo and Y2G dyes in the treatment of the HepG2 cell line. The inhibition percentage started within the first 24 hours and continued throughout the 48th and 72nd hours as long as the application continued as given in Figure 4a and 4b (Kalındemirtaş, 2022). It is quite clear from the result presented here that presence of vitamin C with both indigo and Y2G dyes had a positive effect on HepG2 proliferation.…”

Section: Resultssupporting

confidence: 66%

Cytotoxic Efficacy of Indigo and Yellow 2G with Vitamin C on the HepG2 Cell Line

Karabörk

Okçu

PEKDEMİR

2023

International Journal of Nature and Life Sciences

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We aimed to examine the effect of indigo and yellow 2G (Y2G) dyes, that are also commonly used as food additive and colorant, individually and combination with vitamin C on HepG2 human liver carcinoma cell line proliferation in vitro. MTT assay was applied to investigate the effect of dyes individually combined with vitamin C on HepG2 cells by treatment with different concentrations of these dyes for varying exposure times and performed. Regarding the exposure of HepG2 cells to indigo and Y2G dyes for 24, 48 and 72 h, 50 and 500 µg/ml (p=0.001 and p=0.003 respectively) of indigo at 24 h and 500 µg/ml (p=0.012) at 72 h; it showed that 5 µg/ml of Y2G for 24 h (p=0.004) and 1 µg/ml for 48 and 72 h (p=0.000, p=0.001) had the highest cytotoxic effect. Also, the results indicated that, especially the combinations of “indigo + vitamin C“ significantly increased cytotoxic effects on the HepG2 cell line (p=0.003). The obtained findings provide an exciting insight into the cytotoxic effects of indigo on HepG2 cells, and how they might work together to combat HCC's aggression.

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Section: Resultssupporting

confidence: 66%

Cytotoxic Efficacy of Indigo and Yellow 2G with Vitamin C on the HepG2 Cell Line

Karabörk

Okçu

PEKDEMİR

2023

International Journal of Nature and Life Sciences

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“…The concentration of AA (50 µg/mL) was previously proved to be effective for stimulating collagen synthesis in human fibroblasts [ 26 ]. According to the literature, we reported that the same concentration of AA does not interfere with the cancer cell viability in the 3D CRC Day4 µTs with AA [ 27 ], which also allowed for an increase in ECM production by day 12 of culturing. Since it is known that tumor ECM synthesis increases and collagen fibers are upregulated in several types of cancer [ 28 ], we hypothesized that the significant increase in the collagen synthesis by day 12 of culturing was due to cancer cells that act on continuous ECM remodeling to create a microenvironment that promotes tumorigenesis [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Colorectal Cancer Bioengineered Microtissues as a Model to Replicate Tumor-ECM Crosstalk and Assess Drug Delivery Systems In Vitro

Rocca

Gregorio

Lagreca

et al. 2023

IJMS

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Current 3D cancer models (in vitro) fail to reproduce complex cancer cell extracellular matrices (ECMs) and the interrelationships occurring (in vivo) in the tumor microenvironment (TME). Herein, we propose 3D in vitro colorectal cancer microtissues (3D CRC μTs), which reproduce the TME more faithfully in vitro. Normal human fibroblasts were seeded onto porous biodegradable gelatin microbeads (GPMs) and were continuously induced to synthesize and assemble their own ECMs (3D Stroma μTs) in a spinner flask bioreactor. Then, human colon cancer cells were dynamically seeded onto the 3D Stroma μTs to achieve the 3D CRC μTs. Morphological characterization of the 3D CRC μTs was performed to assess the presence of different complex macromolecular components that feature in vivo in the ECM. The results showed the 3D CRC μTs recapitulated the TME in terms of ECM remodeling, cell growth, and the activation of normal fibroblasts toward an activated phenotype. Then, the microtissues were assessed as a drug screening platform by evaluating the effect of 5-Fluorouracil (5-FU), curcumin-loaded nanoemulsions (CT-NE-Curc), and the combination of the two. When taken together, the results showed that our microtissues are promising in that they can help clarify complex cancer–ECM interactions and evaluate the efficacy of therapies. Moreover, they may be combined with tissue-on-chip technologies aimed at addressing further studies in cancer progression and drug discovery.

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Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells

Cited by 2 publications

References 0 publications

Cytotoxic Efficacy of Indigo and Yellow 2G with Vitamin C on the HepG2 Cell Line

Cytotoxic Efficacy of Indigo and Yellow 2G with Vitamin C on the HepG2 Cell Line

Colorectal Cancer Bioengineered Microtissues as a Model to Replicate Tumor-ECM Crosstalk and Assess Drug Delivery Systems In Vitro

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