Cytotoxic and antimicrobial activity of selected Cameroonian edible plants

Abstract

BackgroundIn Cameroon, the use of edible plants is an integral part of dietary behavior. However, evidence of the antimicrobial as well as the cytotoxic effects of many of them has not been investigated. In the present study, aqueous and methanol extracts from barks, seeds, leaves and roots of three Cameroonian edible plants namely Garcina lucida, Fagara heitzii and Hymenocardia lyrata were evaluated for their cytotoxic and antimicrobial activities.MethodsAntibacterial and antifungal activities were assessed by the broth micro-dilution method meanwhile the cytotoxicity was performed using sulphorhodamine B assay (SRB) against the human leukemia THP-1, the alveolar epithelial A549, prostate cancer PC-3, breast adenocarcinoma MCF-7 and cervical cancer HeLa cell lines.ResultsThe minimum inhibitory concentration (MIC) values of the seven tested extracts ranged from 62.5 μg/ml to 1000 μg/ml. The methanol (MeOH) extract from the roots of H. lyrata showed the highest antibacterial activity against Gram-positive bacteria S. aureus and S. epidermitis. The best antifungal activity was obtained with the MeOH extract from the leaves of G. lucida against C. tropicalis (MIC value of 62.5 μg/ml). The in vitro antiproliferative activity revealed that, extract from the bark of F. heitzii and extract from H. lyrata roots had significant cytotoxic activity on THP-1 (IC50 8.4 μg/ml) and PC-3 (IC50 9.5 μg/ml) respectively.ConclusionOur findings suggest that Cameroonian spices herein studied could be potentially useful for the development of therapeutic agents against bacterial infections as well as for prostate and leukemia cancer.

Leucas mollissima, a Source of Bioactive Compounds with Antimalarial and Antimycobacterium Activities

“…25 177) using the XTT Reduction Menadione Assay protocol and M. bovis (ATCC No. 35 745) using the Nitrate Reductase assay protocol [19][20][21]. Protocol details are given in Supporting Information.…”

Design and synthesis of 11α-substituted bile acid derivatives as potential anti-tuberculosis agents

“…In a preliminary screening, the antimycobacterial activity of these compounds was assessed at concentrations of 30, 10, 3 μg/mL using XRMA antitubercular screening protocol. 19,20 The antimycobacterial activity results are summarized in Table 1.…”

“…Compounds 2b, 3, 7, and 8 tested in this study exhibited high antitubercular activities against both dormant and active stages of MTB under both in-vitro and ex-vivo conditions. Minimum Inhibitory Concentration (MIC) values of the most active compound (2b) determined against the dormant state were 2.43 and 1.55 μg/mL respectively when measured under invitro 19,20 and ex-vivo 21 conditions. Interestingly, compound 2b was found to be almost equally effective against the actively growing MTB bacilli.…”

A new butenolide cinnamate and other biological active chemical constituents fromPolygonum glabrum

“…Anti-proliferative activity against THP-1, A549, PANC-1, HeLa and MCF-7 cell lines using MTT assay Effect of the extract (PGM) and compounds 1 -7 were evaluated on cell growth in acute monocytic leukaemia cell line THP-1, lung A549 adenocarcinoma, pancreatic PANC-1 adenocarcinoma, cervix adenocarcinoma HeLa and MCF7 human mammary gland/breast adenocarcinoma epithelial cell line. In vitro cytotoxicity of all test samples were assessed on a panel of five human cancer cell lines using a standard MTT assay for measuring cellular proliferation (Mosmann 1983;Ciapetti et al 1993;Dzoyem et al 2013). Each concentration was tested in triplicates in a single experiment.…”

“…All experiments were performed in triplicates and IC 50 and MIC values were calculated from their dose -response curves (Figures S9 -S11) (Singh et al 2011;Dzoyem et al 2013 (Table S3).…”