Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae)

Katoch, Meenu; Singh, Gurpreet; Sharma, Sadhna; Gupta, Nidhi; Sangwan, Payare L.; Saxena, Ajit Kumar

doi:10.1186/1472-6882-14-52

Cited by 53 publications

(32 citation statements)

References 36 publications

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“…; Katoch et al . ). From a few native medicinal plants, our research team has also reported some bioactive endophytes synthesizing chief metabolites (Kour et al .…”

Section: Discussionmentioning

confidence: 97%

Discovery of anti-microbial and anti-tubercular molecules from Fusarium solani : an endophyte of Glycyrrhiza glabra

et al. 2017

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Aims Glycyrrhiza glabra is a high‐value medicinal plant thriving in biodiversity rich Kashmir Himalaya. The present study was designed to explore the fungal endophytes from G. glabra as a source of bioactive molecules. Methods and Results The extracts prepared from the isolated endophytes were evaluated for anti‐microbial activities using broth micro‐dilution assay. The endophytic strain coded as A2 exhibiting promising anti‐bacterial as well as anti‐tuberculosis activity was identified as Fusarium solani by ITS‐5.8S ribosomal gene sequencing technique. This strain was subjected to large‐scale fermentation followed by isolation of its bioactive compounds using column chromatography. From the results of spectral data analysis and comparison with literature, the molecules were identified as 3,6,9‐trihydroxy‐7‐methoxy‐4,4‐dimethyl‐3,4‐dihydro‐1H‐benzo[g]isochromene‐5,10‐dione (1), fusarubin (2), 3‐O‐methylfusarubin (3) and javanicin (4). Compound 1 is reported for the first time from this strain. All the four compounds inhibited the growth of various tested bacterial strains with MIC values in the range of <1 to 256 μg ml−1. Fusarubin showed good activity against Mycobacterium tuberculosis strain H37Rv with MIC value of 8 μg ml−1, whereas compounds 1, 3 and 4 exhibited moderate activity with MIC values of 256, 64, 32 μg ml−1, respectively. Conclusions To the best of our knowledge, this is the first study that reports significant anti‐tuberculosis potential of bioactive molecules from endophytic F. solani evaluated against the virulent strain of M. tuberculosis. This study sets background towards their synthetic intervention for activity enhancement experiments in anti‐microbial drug discovery programme. Significance and Impact of the Study Due to the chemoprofile variation of same endophyte with respect to source plant and ecoregions, further studies are required to explore endophytes of medicinal plants of all unusual biodiversity rich ecoregions for important and or novel bioactive molecules.

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“…; Katoch et al . ). From a few native medicinal plants, our research team has also reported some bioactive endophytes synthesizing chief metabolites (Kour et al .…”

Section: Discussionmentioning

confidence: 97%

Discovery of anti-microbial and anti-tubercular molecules from Fusarium solani : an endophyte of Glycyrrhiza glabra

et al. 2017

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“…A similar diversity pattern has been reported in many plants such as lima bean, Theobroma cacao , and Coccoloba cereifera (López‐González et al , ). The results indicate that the isolated endophytic communities differ between sampling sites and tissues due to host specificity and their interaction with different ecological niches (Katoch et al , ).…”

Section: Discussionmentioning

confidence: 99%

Diversity, plant growth‐promoting traits, and biocontrol potential of fungal endophytes of Sorghum bicolor

et al. 2020

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The diversity of fungal endophytes in Sorghum bicolor was investigated in samples collected from 10 different geographical regions of Karnataka state, India. A total of 360 endophytes were isolated from leaf, stem, and root tissues and were assigned to 26 fungal species based on morphology and molecular characterization using ITS sequences. All the endophytes belonged to the phylum Ascomycota. The diversity (Shannon H, 2.57; Simpson_1‐D, 0.92) and species richness (Margalef's, 4.68; Menhinick, 3.61) were found to be higher for the endophytes isolated from leaf tissues. The species evenness of the endophytic assemblage was strongly influenced by tissue type, followed by geographical location. The biocontrol potential of isolated endophytes was evaluated against economically destructive sorghum grain mould pathogens such as Fusarium thapsinum, Epicoccum sorghinum, Alternaria alternata, and Curvularia lunata using the dual culture method. Biocontrol potential was exhibited by 26 endophytic isolates, of which Trichoderma asperellum recorded broad‐spectrum activity against target pathogens, followed by E. nigrum and A. longipes. Most (82%) endophytes showed plant growth‐promoting traits. Biosynthesis of indole‐3‐acetic acid (IAA) was observed in 84% of isolates, and phosphate solubilization, siderophore production, and cellulase activity was observed in 69%, 23%, and 27% of isolates, respectively. Seeds treated with T. asperellum exhibited a significantly higher seed vigour index (2096), germination percentage (94%), and yield under greenhouse and field conditions. The results were substantiated by the confocal microscopy analysis, which clearly demonstrated the colonization of treated endophyte in root tissues. The present study reveals an ecofriendly approach to explore T. asperellum in sorghum disease management.

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“…The results revealed MIC of 15.6, 62.5, 15.6 and 62.5 lg/mL against S. aureus, B. subtilis, S. typhi and E. coli, respectively (Figure 4(A)). Likewise, ethyl acetate extract of endophytic fungi from Bacopa monnieri displayed antimicrobial activity against B. subtilis, P. aeruginosa, S. typhimurium, E. coli, K. pneumonia and S. aureus with MIC of 10-100 lg/mL (Katoch et al 2014). Also, Ratnaweera et al (2015) and Dissanayake et al (2016) have reported that endophytic fungi Fusarium sp.…”

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Murali

Mahendra

Hema

et al. 2017

Pharmaceutical Biology

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Context: Sida acuta Burm.f. (Malvaceae) extracts are reported to have applications against malaria, diuretic, antipyretic, nervous and urinary diseases. No fungal endophytes of S. acuta are reported. Objective: Isolation, identification and evaluation of antibacterial, antioxidant, anticancer and haemolytic potential of fungal endophytes from the ethnomedcinal plant S. acuta. Materials and methods: Sida acuta stem segments were placed on PDA medium to isolate endophytic fungi. The fungus was identified by genomic DNA analysis and phylogenetic tree was constructed using ITS sequences (GenBank) to confirm species. The antibacterial efficacy of Aspergillus sulphureus MME12 ethyl acetate extract was tested against Gram-positive and Gram-negative pathogenic bacteria. DPPH free radical scavenging activity, anticancer and DNA fragmentation against EAC cells, and direct haemolytic activity (100-500 lg/mL) using human erythrocytes were determined. Results and discussion: The ethyl acetate extract of A. sulphureus (Fresen.) Wehmer (Trichocomaceae) demonstrated significant antibacterial potential against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi compared to streptomycin. MIC against test pathogens was in the range of 15.6-62.5 lg/mL. The antioxidant results revealed significant RSA from 12.43% to 62.02% (IC 50 ¼ 350.4 lg/ mL, p 0.05). MME12 offered considerable inhibition of EAC proliferation (23% to 84%, IC 50 ¼ 216.7 lg/mL, p 0.05) supported by DNA fragmentation studies. The extract also offered insignificant haemolysis (5.6%) compared to Triton X-100. Conclusions: A single endophytic fungus, A. sulphureus MME12 was isolated and identified using molecular profiling. The above-mentioned findings support the pharmacological application of A. sulphureus MME12 extract and demand for purification of the active principle(s).

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Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae)

Cited by 53 publications

References 36 publications

Discovery of anti-microbial and anti-tubercular molecules from Fusarium solani : an endophyte of Glycyrrhiza glabra

Discovery of anti-microbial and anti-tubercular molecules from Fusarium solani : an endophyte of Glycyrrhiza glabra

Diversity, plant growth‐promoting traits, and biocontrol potential of fungal endophytes of Sorghum bicolor

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

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