Cytotoxic Activity of the Methanolic Extract of <i>Turnera diffusa</i> Willd on Breast Cancer Cells

Avelino-Flores, María del Carmen; Cruz-López, María del Carmen; Jiménez-Montejo, Fabiola E.; Reyes‐Leyva, Julio

doi:10.1089/jmf.2013.0055

Cited by 23 publications

(14 citation statements)

References 35 publications

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“…Regarding the cytotoxic effect of apigenin on HeLa cells, our study showed lower IC 50 values than others, with reported IC 50 values of 40 μ M at 30 h [ 39 ] and 37 μ M at 24 h [ 41 ]. We have knowledge of only one study that investigated the cytotoxic activity of apigenin on SiHa and C33A cells [ 64 ] that used the same concentrations and times used here (0–100 μ M at 72 h). The results from that study are similar to ours: apigenin was cytotoxic for both SiHa and C33A cells with IC 50 values of 50.14 μ M and 45.10 μ M, respectively.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Triggered by Apigenin Induces Apoptosis in a Comprehensive Panel of Human Cervical Cancer‐Derived Cell Lines

Souza

Mendonca

Gimenes

et al. 2017

Oxidative Medicine and Cellular Longevity

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Recently, the cytotoxic effects of apigenin (4′,5,7-trihydroxyflavone), particularly its marked inhibition of cancer cell viability both in vitro and in vivo, have attracted the attention of the anticancer drug discovery field. Despite this, there are few studies of apigenin in cervical cancer, and these studies have mostly been conducted using HeLa cells. To evaluate the possibility of apigenin as a new therapeutic candidate for cervical cancer, we evaluated its cytotoxic effects in a comprehensive panel of human cervical cancer-derived cell lines including HeLa (human papillomavirus/HPV 18-positive), SiHa (HPV 16-positive), CaSki (HPV 16 and HPV 18-positive), and C33A (HPV-negative) cells in comparison to a nontumorigenic spontaneously immortalized human epithelial cell line (HaCaT). Our results demonstrated that apigenin had a selective cytotoxic effect and could induce apoptosis in all cervical cancer cell lines which were positively marked with Annexin V, but not in HaCaT (control cells). Additionally, apigenin was able to induce mitochondrial redox impairment, once it increased ROS levels and H2O2, decreased the Δψm, and increased LPO. Still, apigenin was able to inhibit migration and invasion of cancer cells. Thus, apigenin appears to be a promising new candidate as an anticancer drug for cervical cancer induced by different HPV genotypes.

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress Triggered by Apigenin Induces Apoptosis in a Comprehensive Panel of Human Cervical Cancer‐Derived Cell Lines

Souza

Mendonca

Gimenes

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…The other chemical classes evaluated had moderate presence in the evaluated extracts. Studies already reported in the literature show that arbutin and apigenin flavonoids are the main chemical constituents of Turnera diffusa with cytotoxic activity, present in methanolextract (AVELINO-FLORES et al, 2015).…”

Section: Resultsmentioning

confidence: 92%

PHYTOCHEMICAL ANALYSIS OF Turnera diffusa WILLD

Silva

Santos

Nunes

et al. 2020

Int J Innov Educ Res

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Turnera diffusa WILLD is a plant used in folk medicine as a natural stimulant, and the objective of this research is to perform the phytochemical analysis of the secondary metabolites of the damiana of occurrence in the Juazeiro region, northern Bahia - Brazil. A phytochemical screening was performed from three Crude Ethanol Extracts (BSE), EEB1: leaves and flowers; EEB2: stems and EEB3: root. A thin analytical layer chromatography was performed using specific developers to detect each chemical class. Alkaloids, coumarins, anthranic derivatives, phenolic compounds, mono, sequi and diterpenes, naphthoquinones, triterpenes and steroids, saponins, hydrolyzed tannins and xanthines were identified in the three extracts. Only in the BSE of leaves and flowers observed the presence of antroquinones and aglycones. The secondary metabolites identified in the study evidence the pharmacological potential of Turnera diffusa. Phytochemical screening concluded the presence of fourteen classes of secondary metabolites in damiana, indicating important medicinal potential with pharmacological effects reported in the literature.

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“…Its traditional use dates back to ancient Mayan and Mexican indigenous groups that prepared medicinal beverage for giddiness treatment and as an aphrodisiac [24,25]. Currently, several damiana products have been commercially available for their use as aphrodisiac, diuretic, and cancer and diabetes treatments [26,27]. T. difussa extracts contain more than 47 organic compounds comprised of flavonoids, terpenoids, saccharides, phenolics, and cyanogenic derivatives [28].…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of gold nanoparticles using Damiana induce antiseptic activity against gram-negative bacteria

Hernández‐Adame¹,

Ruvalcaba²,

Ruiz‐Gómez³

et al. 2020

Preprint

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Interest in green synthesis methods for nanoparticle production with potential use in biology, pharmacy, and medicine is a forefront field in research. Among the routes described in the literature, the use of plant extracts as reducing and capping agents is one of the most promising. Turnera diffusa commonly called Damiana is a wild shrub traditionally used as aphrodisiac, diuretic, and for cancer and diabetes treatments. Its extracts are rich in flavonoid, phenolic, terpenoid, and other active compounds that possess antiallergic, antiviral, and antifungal activities. Besides, flavonoids have been reported to have a redox potential sufficient for metal ion reduction. Thus, this study reports a novel and facile method for green gold nanoparticle (AuNPs) synthesis using T. diffusa extracts. The samples were fully characterized by scanning electron microscopy, acrylonitrile butadiene styrene ultraviolet-visible, dynamic light scattering, X-ray photoelectron, and inductively coupled plasma optical emission spectroscopies. The results showed high efficiency of T. diffusa extracts to reduce and stabilize the AuNPs which showed spherical shape, 24 nm diameter, negative surface charge, and high colloidal stability at biological pH and alkaline media. Additionally, its antimicrobial activity was evaluated showing high antiseptic capacity against gram-negative Salmonella enteritis and Escherichia coli ATCC 8739 bacteria.

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Cytotoxic Activity of the Methanolic Extract of Turnera diffusa Willd on Breast Cancer Cells

Cited by 23 publications

References 35 publications

Oxidative Stress Triggered by Apigenin Induces Apoptosis in a Comprehensive Panel of Human Cervical Cancer‐Derived Cell Lines

Oxidative Stress Triggered by Apigenin Induces Apoptosis in a Comprehensive Panel of Human Cervical Cancer‐Derived Cell Lines

PHYTOCHEMICAL ANALYSIS OF Turnera diffusa WILLD

Green synthesis of gold nanoparticles using Damiana induce antiseptic activity against gram-negative bacteria

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