Cytotoxic activity of poly-ɛ-caprolactone lipid-core nanocapsules loaded with lycopene-rich extract from red guava (Psidium guajava L.) on breast cancer cells

Vasconcelos, Andreanne Gomes; Valim, Martina O.; Amorim, Adriany G. N.; Amaral, Constança Pais do; Almeida, Miguel Peixoto de; Borges, Tatiana Karla dos Santos; Socodato, Renato; Portugal, Camila C.; Brand, Guilherme D.; Mattos, Jacó S. C.; Relvas, João B.; Plácido, Alexandra; Eaton, Peter; Ramos, Doralina A.R.; Kückelhaus, Selma Aparecida Souza; Leite, J. R.

doi:10.1016/j.foodres.2020.109548

Cited by 32 publications

(31 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context, nanobiotechnology has been an innovative alternative to improve the stability and biological activities of this compound. For example, lipid-core nanocapsules with a polysorbate 80-coated poly-ɛcaprolactone wall efficiently stabilize LEG for 7 months and improve its cytotoxic activity on breast cancer cells (Vasconcelos et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Promising self-emulsifying drug delivery system loaded with lycopene from red guava (Psidium guajava L.): in vivo toxicity, biodistribution and cytotoxicity on DU-145 prostate cancer cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Self-emulsifying drug delivery systems (SEDDSs) have attracted attention because of their effects on solubility and bioavailability of lipophilic compounds. Herein, a SEDDS loaded with lycopene purified from red guava (nanoLPG) was produced. The nanoemulsion was characterized using dynamic light scattering (DLS), zeta potential measurement, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), lycopene content quantification, radical scavenging activity and colloidal stability in cell culture medium. Then, in vivo toxicity and tissue distribution in orally treated mice and cytotoxicity on human prostate carcinoma cells (DU-145) and human peripheral blood mononuclear cells (PBMC) were evaluated. Results NanoLPG exhibited physicochemical properties with a size around 200 nm, negative zeta-potential, and spherical morphology. The size, polydispersity index, and zeta potential parameters suffered insignificant alterations during the 12 month storage at 5 °C, which were associated with lycopene stability at 5 °C for 10 months. The nanoemulsion showed partial aggregation in cell culture medium at 37 °C after 24 h. NanoLPG at 0.10 mg/mL exhibited radical scavenging activity equivalent to 0.043 ± 0.002 mg Trolox/mL. The in vivo studies did not reveal any significant changes in clinical, behavioral, hematological, biochemical, and histopathological parameters in mice orally treated with nanoLPG at 10 mg/kg for 28 days. In addition, nanoLPG successfully delivered lycopene to the liver, kidney and prostate in mice, improved its cytotoxicity against DU-145 prostate cancer cells—probably by pathway independent on classical necrosis and apoptosis—and did not affect PBMC viability. Conclusions Thus, nanoLPG stands as a promising and biosafe lycopene delivery system for further development of nanotechnology-based health products. Graphical Abstract

show abstract

Section: Introductionmentioning

confidence: 99%

Promising self-emulsifying drug delivery system loaded with lycopene from red guava (Psidium guajava L.): in vivo toxicity, biodistribution and cytotoxicity on DU-145 prostate cancer cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…In vitro cultivation of 0.5-3 µg/mL lycopene in several human cancer cell lines showed an inhibitory effect of HT-29 cells with 20% and T84 cells with 30%, after 48 h of cultivation, due to its internalization and induction of changes in the cell cycle, but no effect on other cell types (A549, DU145, HepG2, Hela, Hep-2) [27]. A lycopene-rich extract of red guava, encapsulated in 200 nm lipid nanoparticles or not, showed good cytocompatibility in NIH-3 T3 normal mice fibroblasts and decreased the viability of MCF-7 human adenocarcinoma cells down to 30%, at 100 µg/mL, thus indicating antiproliferative activity, while protecting healthy cells [28]. No studies on the antiproliferative activity of lycopene encapsulated in a protein-polysaccharide complex were found.…”

Section: Antiproliferative Activity Of the Microencapsulated Powdersmentioning

confidence: 98%

Supercritical CO2 Extraction and Microencapsulation of Lycopene-Enriched Oleoresins from Tomato Peels: Evidence on Antiproliferative and Cytocompatibility Activities

et al. 2021

View full text Add to dashboard Cite

Tomato peels are used as a valuable material to extract lycopene-rich oleoresins by supercritical CO2 extraction. The extraction involves continuous circling of CO2 to the extractor after removing the solute in the separators, S40 and S45, where the solvent power of the CO2 is reduced by reducing pressure down to 20 MPa in S40 and 5 MPa in S45, respectively, leading to two extracts. Lycopene is found to be the major compound, representing 93% and 76% of the total carotenoids in S40 and S45 extracts, respectively. The two extracts are microencapsulated in whey protein concentrate and acacia gum by complex coacervation and freeze-drying, leading to corresponding P40 and P45 powders, with antioxidant activity of 8.57 ± 0.74 and 9.37 ± 0.48 mMol TEAC/g DW in P40 and P45, respectively. Different structural and morphological patterns are observed, with finer microparticles of 1–2 µm in P45. Both powders show dose and time-dependent antiproliferative activity. The half-maximal inhibitory concentration values are 100 µg/mL for P40 and 750 µg/mL for P45 sample, indicating a higher antiproliferative effect of P40 over P45 in HT-29 cell culture. The powders have an extended range of cytocompatibility, up to 1000 µg/mL, in L929 normal cells, stimulating the cell growth. Lycopene retention is tested, and values of 48% and 29% in P40 and P45 are found after 21 days at 25 °C, with the degradation rate in P45 significantly higher, due to the higher content of the surface lycopene, which favored its degradation.

show abstract

“…However, no biological activity was determined for the PCL-lycopene based NPs. In a recent study dealing with preparation of PCL-lycopene NPs from red guava extract ( Psidium guajava L.), Vasconcelos, et al [ 45 ] demonstrated their enhanced stability and inhibitory effects on breast cancer cell MCF-7 as well as alleviation of LPS-induced oxidative stress in microglial cells HMC3 by using a time-lapse microscopy. More elaborately, an organic phase containing PCL in acetone, sorbitan monostearate, coconut oil and lycopene extract in ethanol at a ratio of 60.81:14.77:0.10:1.74 was mixed with 190 mL of an aqueous phase containing Tween 80 (150 μL), followed by stirring at 40 °C for 10 min and evaporating under reduced pressure to 10 mL at 37 °C.…”

Section: Preparation Physicochemical Characterization Stability Evaluation and Biological Activitymentioning

confidence: 99%

“…NPs = nanoparticles. Adapted with permission from Kodama, et al [ 75 ], Monteiro, et al [ 76 ], Rehman, et al [ 9 ], and Vasconcelos, et al [ 45 ].…”

Section: Figurementioning

confidence: 99%

Recent Advances on Nanoparticle Based Strategies for Improving Carotenoid Stability and Biological Activity

2021

View full text Add to dashboard Cite

Carotenoids are natural pigments widely used in food industries due to their health-promoting properties. However, the presence of long-chain conjugated double bonds are responsible for chemical instability, poor water solubility, low bioavailability and high susceptibility to oxidation. The application of a nanoencapsulation technique has thus become a vital means to enhance stability of carotenoids under physiological conditions due to their small particle size, high aqueous solubility and improved bioavailability. This review intends to overview the advances in preparation, characterization, biocompatibility and application of nanocarotenoids reported in research/review papers published in peer-reviewed journals over the last five years. More specifically, nanocarotenoids were prepared from both carotenoid extracts and standards by employing various preparation techniques to yield different nanostructures including nanoemulsions, nanoliposomes, polymeric/biopolymeric nanoparticles, solid lipid nanoparticles, nanostructured lipid nanoparticles, supercritical fluid-based nanoparticles and metal/metal oxide nanoparticles. Stability studies involved evaluation of physical stability and/or chemical stability under different storage conditions and heating temperatures for varied lengths of time, while the release behavior and bioaccessibility were determined by various in vitro digestion and absorption models as well as bioavailability through elucidating pharmacokinetics in an animal model. Moreover, application of nanocarotenoids for various biological applications including antioxidant, anticancer, antibacterial, antiaging, cosmetics, diabetic wound healing and hepatic steatosis were summarized.

show abstract

Cytotoxic activity of poly-ɛ-caprolactone lipid-core nanocapsules loaded with lycopene-rich extract from red guava (Psidium guajava L.) on breast cancer cells

Cited by 32 publications

References 72 publications

Promising self-emulsifying drug delivery system loaded with lycopene from red guava (Psidium guajava L.): in vivo toxicity, biodistribution and cytotoxicity on DU-145 prostate cancer cells

Promising self-emulsifying drug delivery system loaded with lycopene from red guava (Psidium guajava L.): in vivo toxicity, biodistribution and cytotoxicity on DU-145 prostate cancer cells

Supercritical CO2 Extraction and Microencapsulation of Lycopene-Enriched Oleoresins from Tomato Peels: Evidence on Antiproliferative and Cytocompatibility Activities

Recent Advances on Nanoparticle Based Strategies for Improving Carotenoid Stability and Biological Activity

Contact Info

Product

Resources

About