Cytotoxic activity of lymphoid cells from mice immunized with semiallogeneic hybrid cells: Requirement of in vitro lymphoid cells culture for expression of cytotoxicity against a syngeneic chemically induced tumor

Payelle, B; Goguel, A; Poupon, Marie‐France; Lespinats, G

doi:10.1016/0008-8749(82)90038-7

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…MCB6-1 tumor cells alone. immunity can be transferred by T lymphocytes against the parental fibrosarcoma (Payelle et af., 1981); these immune T lymphocytes are not directly cytotoxic in vitro against the tumor cells but they acquire a cytotoxic activity after a 4 d culture (Payelle et al, 1982). The time of development of the two phases of immunity may vary according to the experiment, but it is always apparent.…”

Section: Protocol Of Experiments Expressed In Figurementioning

confidence: 99%

Role of T suppressor cells in the cycling of the immune response against a murine fibrosarcoma

Payelle

Lespinats

Tlouzeau

1984

Intl Journal of Cancer

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Antitumor immunity against a fibrosarcoma in C57BL/6 mice was obtained by means of a semi-allogenic somatic hybrid cell derived from the fusion of this C57BL/6 fibrosarcoma (MCB6-1) and A9 cells of C3H origin. In a Winn assay, this immunity could be transferred by T lymphocytes to normal C57BL/6 recipient mice during an early and a late phase after immunization. There appeared to be a transient non-responsive period during which no immunity could be transferred. Injection of cyclophosphamide (CY) into mice before immunization increased the level of immunity during this period, and reconstitution of animals with normal spleen cells abolished the effect of CY. During the non-responsive period, suppressor cells were demonstrated in the spleen: the i.v. transfer of these suppressor cells to normal mice significantly inhibited the induction of antitumor immunity; the suppressive effect was transferred by T lymphocytes of the Lyt-2+ phenotype. No suppressive effect on antitumor protection was observed when suppressor cells were transferred simultaneously with immune T lymphocytes in the Winn assay. From these findings, it appears that T-suppressor cells regulate the antitumor response, interfering with the afferent (induction) arm of the immune response.

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Section: Protocol Of Experiments Expressed In Figurementioning

confidence: 99%

Role of T suppressor cells in the cycling of the immune response against a murine fibrosarcoma

Payelle

Lespinats

Tlouzeau

1984

Intl Journal of Cancer

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“…2); similar results were obtained with the HindIII fragment (nucleotides 482 to 1934) containing the finger region, but this probe gives a higher background with 28S RNA (data not shown). A strongly hybridizing 3-kilobase mRNA was detectable in cells transformed by 3methylcholanthrene (MCA16 and MCB6-1 cells; 26,36) or simian virus 40 (SVMKcl9; 39) but was weakly, if at all, detectable in the corresponding untransformed cells (C3H1OT1/2, L, and kidney cells) (Fig. 4A).…”

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confidence: 99%

A Gene That Encodes a Protein Consisting Solely of Zinc Finger Domains Is Preferentially Expressed in Transformed Mouse Cells

Ernoult‐Lange

Kress

Hamer

1990

Molecular and Cellular Biology

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We describe the cloning and characterization of the mouse MOK-2 gene, a new member of the Krüppel family of zinc finger proteins. Sequencing of both cDNA and genomic clones showed that the predicted MOK-2 protein consists of seven zinc finger domains with only five additional amino acids. The finger domains of MOK-2 are highly homologous to one another but not to those of other zinc finger proteins. MOK-2 is preferentially expressed in transformed cell lines, brain tissue, and testis tissue. Its possible role in cellular transformation is discussed.

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Deficient tumor‐specific immunity in old mice: in vivo mediation by suppressor cells, and correction of the defect by interleukin 2 supplementation in vitro but not in vivo

Bruley-Rosset

Payelle

1987

Eur J Immunol

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The capacity of old (18-24 months) C57BL/6 mice to develop an immune reaction against MC-B6-1 fibrosarcoma cells was studied using in vivo adoptive transfer experiments (Winn assay) and in vitro T cell-mediated cytotoxicity test. Anti-tumor immunity was found to decline with age, as indicated by a decreased anti-tumor growth T cell activity. A suppressive activity was also found present in the splenic T cell population of old mice which can inhibit the in vivo generation of immune T cells in young mice. These suppressors, or their precursors, were resistant to cyclophosphamide treatment and were effective only when administered 3 days before the immunization of young mice. These mice developed immune T cells perfectly when the suppressors were administered 3 days after immunization, indicating that suppressors may act at an early phase of T cell activation. The protective activity of T cells in vivo correlated well with the in vitro T cell cytotoxicity for MC-B6-1 tumor cells, as both were depressed in old mice. Exogenous interleukin 2 (IL 2) addition during the 4-day culture period partially restored the low cytotoxic activity of old immunized lymphocytes, suggesting that specific clones were present but that a lack of IL 2 limited their expansion. However, in vivo supplementation with IL 2 administered after immunization did not increase the protection mediated by old immunized T cells but, rather, increased the suppression. This work demonstrates the presence of a T cell suppressive activity in the spleen of old mice but also indicates that precursors of cytotoxic cells are generated by the immunization. It seems that in vitro IL 2 addition increases cytotoxic cells while in vivo IL 2 administration amplifies the development of suppressor cells generated during immunization of aged mice.

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Cytotoxic activity of lymphoid cells from mice immunized with semiallogeneic hybrid cells: Requirement of in vitro lymphoid cells culture for expression of cytotoxicity against a syngeneic chemically induced tumor

Cited by 5 publications

References 23 publications

Role of T suppressor cells in the cycling of the immune response against a murine fibrosarcoma

Role of T suppressor cells in the cycling of the immune response against a murine fibrosarcoma

A Gene That Encodes a Protein Consisting Solely of Zinc Finger Domains Is Preferentially Expressed in Transformed Mouse Cells

Deficient tumor‐specific immunity in old mice: in vivo mediation by suppressor cells, and correction of the defect by interleukin 2 supplementation in vitro but not in vivo

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