Cytotoxic activity of carotenoid rich fractions from <scp><i>Haematococcus pluvialis</i></scp> and <scp><i>Dunaliella salina</i></scp> microalgae and the identification of the phytoconstituents using LC‐DAD/ESI‐MS

Baz, Farouk K. El; Hussein, Rehab A.; Mahmoud, Khaled; Abdo, Sayeda M.

doi:10.1002/ptr.5976

Cited by 32 publications

(20 citation statements)

References 23 publications

(30 reference statements)

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“…In recent years, natural carotenoids have health benefits that attract attention, and these benefits were gained from carotenes and xanthophylls that have the ability to scavenge oxygen radicals [12]. Microalgae are the richest sources of natural carotenoids, especially strains of Chlorophyta such as Dunaliella salina (D. salina) which is a biflagellate unicellular green alga [13][14][15]. e algal cells produced three valuable products: β-carotene, glycerol, and proteins.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Dunaliella salina Microalgae on Thioacetamide- (TAA-) Induced Hepatic Liver Fibrosis in Rats: Role of TGF-β and MMP9

El-Baz

Salama

2019

BioMed Research International

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Liver fibrosis represents a serious global health-care problem and is the outcome of many chronic liver diseases, cirrhosis, hepatitis, and toxin accumulation. The present study aimed to evaluate the antifibrotic curative effect of Dunaliella salina (D. salina) on thioacetamide- (TAA-) induced liver fibrosis in rats. Liver fibrosis was induced by TAA (200mg/kg; i.p.) twice per week for 6 weeks. D. salina was given orally (100 and 200 mg/kg) and silymarin was given orally (100 mg/kg) daily, for 4 weeks after TAA. Serum transaminase activities, liver inflammatory cytokines, fibrotic biomarkers, and liver histopathology were assessed. TAA significantly (p<0.05) elevated serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and serum levels of total bilirubin, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and transforming growth factor-beta (TGF-β) with a reduction in albumin. In addition, TAA increased hepatic contents of collagen I, a-smooth muscle actin (α-SMA), and tissue inhibitors of metalloproteinase (TIMP-1), reduced matrix metalloproteinase 9 (MMP9), and finally produced marked degeneration and fibrosis of hepatocytes. Treatment with D. salina or silymarin improved the histological feature of hepatocytes and ameliorated the deleterious effects of TAA in a dose-dependent manner. Based on these results, it could be concluded that the use of D. salina could be assigned for liver fibrosis treatment via its anti-inflammatory and antifibrotic properties.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Dunaliella salina Microalgae on Thioacetamide- (TAA-) Induced Hepatic Liver Fibrosis in Rats: Role of TGF-β and MMP9

El-Baz

Salama

2019

BioMed Research International

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“…Examples of main compounds of this class are ß‐carotene, lycopene, and lutein (Figure ). These compounds may exert various and relevant pharmacological actions partly via antioxidant mechanisms (Adadi, Barakova, & Krivoshapkina, ; Durazzo et al, ; El‐Baz, Hussein, Mahmoud, & Abdo, ; Monforte et al, ; Müller, Caris‐Veyrat, Lowe, & Böhm, ; Petruk, Del Giudice, Rigano, & Monti, ; Zou, Aboshora, Li, Xiao, & Zhang, ). It is worth mentioning the recent review of Ribeiro, Freitas, Silva, Carvalho, and Fernandes (), on antioxidant and pro‐oxidant activities of carotenoids and their oxidation products.…”

Section: Antioxidant Properties Of Fruit‐based Juice: An Overviewmentioning

confidence: 99%

Fruit‐based juices: Focus on antioxidant properties—Study approach and update

Durazzo

Lucarini

Novellino

et al. 2019

Phytotherapy Research

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This paper proposes a perspective literature review of the antioxidant properties in fruit‐based juices. The total antioxidant properties due to compounds such as carotenoids, polyphenolic compounds, flavonoids, and tannins as well as the assessment of interactions between natural active compounds and other food matrix components can be seen as the first step in the study of potential health benefits of fruit‐based juices. A brief summary is given on the significance of antioxidant properties of fruit juices, the conventional methods for antioxidant activity evaluation, and on the newly emerged sample analysis and data interpretation strategies, that is, chemometric analysis based on spectroscopic data. The effect of fruit processing techniques and the addition of ingredients on the antioxidant properties of fruit‐based juices are also discussed.

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“…The solid product obtained after cooling, was filtered off, dried and crystallized from butanol to give (10) white crystals; Yield: 72%; M.p. 269°C; C 27…”

Section: Synthesis Of 2-[2-(3-(4-methoxy-3-methylphenyl) -1h-pyrazol-mentioning

confidence: 99%

“…A positive control which composed of 100 µg/ mL was used as a known cytotoxic natural agent who gives 100% lethality under the same conditions Table 1. [26,27] The absorbance was then measured using a micro plate multi-well reader (Bio-Rad Laboratories Inc., model 3350, Hercules, California, USA) at 595 nm and a reference wavelength of 620 nm. A statistical significance was tested between samples and negative control (cells with vehicle) using independent t-test by SPSS 11 program.…”

Section: Cytotoxic Effect On Human Cell Line (Hepg2)mentioning

confidence: 99%

Synthesis, Cytotoxicity and Molecular Docking of Some Schiff Bases Derived Quinazolinone Bearing Pyrazoline

2019

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O BJECTIVE of our work based on the synthesis of the quinazolinone derivative, 3-amino-2-[3-(4-methoxy-3-methylphenyl)-4,5-dihydro-1H-pyrazol-5-yl]-4(3H)-qunazolinones, and evaluated for cell line. quinazolinone derivatives based on 3-amino-2-[3-(4-methoxy-3methylphenyl)-4,5-dihydro-1H-pyrazol-5-yl]-4(3H)-qunazolinones were designed, synthesized and evaluated for their in vitro cytotoxic activity against the hepatic carcinoma cell line (HepG2), the results revealed that the tested compounds process inhibitory effects on the growth of HepG2 liver cancer cells. 3-amino-2-[3-(4-methoxy-3-methylphenyl)-4,5-dihydro-1H-pyrazol-5-yl]-4(3H)-quinazolinone showed the highest inhibition activity against HepG2 cell line (IC 50 equals 67.8 µg/mL) among the tested compounds. The molecular modelling studies were performed to explore the detailed binding affinity towards the human liver carcinoma HepG2. The obtained results proved that the most active 3-amino-2-[3-(4-methoxy-3-methylphenyl)-4,5-dihydro-1Hpyrazol-5-yl]-4(3H)-quinazolinone could be useful as a template for future design, adaptation and investigation to construct more active qunazolinone analogs. Moreover, compounds were screened for antibacterial activity and none of them showed noteworthy activity.

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Cytotoxic activity of carotenoid rich fractions from Haematococcus pluvialis and Dunaliella salina microalgae and the identification of the phytoconstituents using LC‐DAD/ESI‐MS

Cited by 32 publications

References 23 publications

Therapeutic Effect of Dunaliella salina Microalgae on Thioacetamide- (TAA-) Induced Hepatic Liver Fibrosis in Rats: Role of TGF-β and MMP9

Therapeutic Effect of Dunaliella salina Microalgae on Thioacetamide- (TAA-) Induced Hepatic Liver Fibrosis in Rats: Role of TGF-β and MMP9

Fruit‐based juices: Focus on antioxidant properties—Study approach and update

Synthesis, Cytotoxicity and Molecular Docking of Some Schiff Bases Derived Quinazolinone Bearing Pyrazoline

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