Cytotoxic Activity of Asphodelus Microcarpus as Amain Food of Spalax Leucodon Egyptiacus

Abstract

The present study aimed to know if the food of Spalax leucodon Egyptiacus has a role in its resistance to cancer. In vitro cytotoxic effects of the main food of Spalax leucodon Egyptiacus as a daily meal that was Asphodelus microcarpus which planted in Marsa Matroh governorate Eldabaa region .We use two fractions of the extract of this plant (Ethyl Acetate &Precipitate), cell viability and cytotoxic activities were assessed using The sulforhodamine B( SRB) assay, IC50 values were estimated for the A375 melanoma. We revealed that the IC50 for the A375 melanoma was 83 ug/ml for Ethyl Acetate (EtOAc) fraction, 65ug/ml for Precipitate (PPT) fraction after 48 hours of treatment. Asphodelus microcarpus fraction has considerable cytotoxic effects on the human melanoma cell line , its show effect on cell line A375 human melanoma ,further studies are required to emphasizes the relation between resistant cancer in Spalax leucodon Egyptiacus and its main food.